Bioprocess Engineering Principles
- 3rd Edition - September 27, 2024
- Imprint: Academic Press
- Authors: Ross Carlson, Kate Morrissey, Pauline M. Doran
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 8 2 2 1 9 1 - 4
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 2 1 9 3 - 8
Bioprocess Engineering Principles, Third Edition provides a solid introduction to bioprocess engineering for students with a limited engineering background. The book explai… Read more
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Request a sales quoteBioprocess Engineering Principles, Third Edition provides a solid introduction to bioprocess engineering for students with a limited engineering background. The book explains process analysis from an engineering perspective using worked examples and problems that relate to biological systems. Application of engineering concepts is illustrated in areas of modern biotechnology, such as recombinant protein production, bioremediation, biofuels, drug development, and tissue engineering, as well as microbial fermentation. With new and expanded material, this remains the book of choice for students seeking to move into bioprocess engineering
- Includes more than 350 problems that demonstrate how fundamental principles are applied in areas such as biofuels, bioplastics, bioremediation, tissue engineering, site-directed mutagenesis, recombinant protein production, and drug development, as well as for traditional microbial fermentation
- Provides in-depth treatment of fluid flow, turbulence, mixing, and impeller design, reflecting recent advances in our understanding of mixing processes and their importance in determining the performance of cell cultures
- Focuses on underlying scientific and engineering principles rather than on specific biotechnology applications, providing a sound basis for teaching bioprocess engineering
- Presents new or expanded coverage of such topics as enzyme kinetics, downstream processing, disposable reactors, genetic engineering, and the technology of fermentation
Senior undergraduate students in applied biology, biomedical engineering, or chemical engineering taking final year options in bioprocessing/biotechnology; Industrial practitioners working in biotechnology, pharmaceutical companies, food industries, and those trained in molecular biology and cell manipulation, who need to acquire knowledge on the principles of large scale processing of biological material
- Cover image
- Title page
- Table of Contents
- Copyright
- Preface
- Scope of Textbook
- Acknowledgments
- Part 1: Introduction: Bioprocessing
- Chapter 1. The Field of Bioprocess Engineering
- Abstract
- 1.1 Steps in bioprocess development
- 1.2 Example bioprocesses
- 1.3 Summary of Chapter 1
- References
- Chapter 2. Microbiology and Biochemistry for Engineers
- Abstract
- 2.1 Cells and Organisms
- 2.2 Cellular Composition
- 2.3 Basic Cellular Metabolism
- 2.4 Cellular Nutrients and Growth Medium
- 2.5 Summary of Chapter 2
- References
- Chapter 3. Introduction to Engineering Calculations and Units
- Abstract
- 3.1 Physical Variables, Dimensions, and Units
- 3.2 Units
- 3.3 Force and Weight
- 3.4 Measurement Conventions
- 3.5 Standard Conditions and Ideal Gases
- 3.6 Physical and Chemical Property Data
- 3.7 Stoichiometry
- 3.8 Methods for Checking and Estimating Results
- Summary of Chapter 3
- Problems
- References
- Suggestions for Further Reading
- Part 2: Fundamental Concepts
- Chapter 4. Mass Balances
- Abstract
- 4.1 Thermodynamic Preliminaries
- 4.2 Law of Conservation of Mass
- 4.3 Procedure for Mass Balance Calculations
- 4.4 Mass Balance Worked Examples
- 4.5 Mass Balances With Recycle, Bypass, and Purge Streams
- 4.6 Stoichiometry of Cell Growth and Product Formation
- 4.7 Unsteady-State Mass Balances
- 4.8 Summary of Chapter 4
- Problems
- References
- Suggestions for Further Reading
- Chapter 5. Energy Balances
- Abstract
- 5.1 Basic Energy Concepts
- 5.2 General Energy Balance Equations
- 5.3 Enthalpy Calculation Procedures
- 5.4 Enthalpy Change in Nonreactive Processes
- 5.5 Procedure for Energy Balance Calculations Without Reaction
- 5.6 Energy Balance Worked Examples Without Reaction
- 5.7 Enthalpy Change Due to Reaction
- 5.8 Heat of Reaction for Processes With Biomass Production
- 5.9 Energy Balance Equation for Cell Culture
- 5.10 Cell Culture Energy Balance Worked Examples
- 5.11 Summary of Chapter 5
- Problems
- References
- Suggestions for Further Reading
- Part 3: Physical Processes
- Chapter 6. Fluid Flow
- Abstract
- 6.1 Classification of Fluids
- 6.2 Fluids in Motion
- 6.3 Viscosity
- 6.4 Non-Newtonian Fluids
- 6.5 Rheological Properties of Cell Broths
- 6.6 Factors Affecting Broth Viscosity
- 6.7 Viscosity Measurement
- 6.8 Summary of Chapter 6
- Problems
- References
- Suggestions for Further Reading
- Chapter 7. Mixing
- Abstract
- 7.1 Functions of Mixing
- 7.2 Mixing Equipment
- 7.3 Impellers
- 7.4 Stirrer Power Requirements
- 7.5 Power Input by Gassing
- 7.6 Impeller Pumping Capacity
- 7.7 Suspension of Solids
- 7.8 Mechanisms of Mixing
- 7.9 Assessing Mixing Effectiveness
- 7.10 Scale-Up of Mixing Systems
- 7.11 Improving Mixing in Bioreactors
- 7.12 Multiple Impellers
- 7.13 Effect of Rheological Properties on Mixing
- 7.14 Role of Shear in Stirred Bioreactors
- 7.15 Summary of Chapter 7
- Problems
- References
- Suggestions for Further Reading
- Chapter 8. Bioreactor Heat Transfer
- Abstract
- 8.1 Heat Transfer Equipment
- 8.2 Mechanisms of Heat Transfer
- 8.3 Conduction
- 8.4 Heat Transfer Between Fluids
- 8.5 Design Equations for Heat Transfer Systems
- 8.6 Application of the Design Equations
- 8.7 Hydrodynamic Considerations with Cooling Coils
- 8.8 Summary of Chapter 8
- Problems
- References
- Suggestions for Further Reading
- Chapter 9. Bioreactor Mass Transfer
- Abstract
- 9.1 Molecular Diffusion
- 9.2 Role of Diffusion in Bioprocessing
- 9.3 Film Theory
- 9.4 Convective Mass Transfer
- 9.5 O2 Uptake in Cell Cultures
- 9.6 Factors Affecting O2 Transfer in Bioreactors
- 9.7 Measuring Dissolved O2 Concentration
- 9.8 Estimating O2 Solubility
- 9.9 Mass Transfer Correlations for O2 Transfer
- 9.10 Measurement of kLa
- 9.11 Measurement of the Specific O2 Uptake Rate, qO2
- 9.12 Practical Aspects of O2 Transfer in Large Bioreactors
- 9.13 Alternative Methods for Oxygenation Without Sparging
- 9.14 O2 Transfer in Shake Flasks
- 9.15 Summary of Chapter 9
- Problems
- References
- Suggestions for Further Reading
- Part 4: Reactions
- Chapter 10. Homogeneous Reactions
- Abstract
- 10.1 Basic Reaction Theory
- 10.2 Calculation of Reaction Rates From Experimental Data
- 10.3 General Reaction Kinetics for Biological Systems
- 10.4 Determining Enzyme Kinetic Constants From Batch Data
- 10.5 Regulation of Enzyme Activity
- 10.6 Kinetics of Enzyme Deactivation
- 10.7 Yields in Cell Culture
- 10.8 Cell Growth Kinetics
- 10.9 Growth Kinetics With Plasmid Instability
- 10.10 Production Kinetics in Cell Culture
- 10.11 Kinetics of Substrate Uptake in Cell Culture
- 10.12 Effect of Culture Conditions on Cell Kinetics
- 10.13 Determining Cell Kinetic Parameters From Batch Data
- 10.14 Effect of Maintenance on Yields
- 10.15 Kinetics of Cell Death
- 10.16 Summary of Chapter 10
- Problems
- References
- Suggestions for Further Reading
- Chapter 11. Heterogeneous Reactions
- Abstract
- 11.1 Heterogeneous Reactions in Bioprocessing
- 11.2 Concentration Gradients and Reaction Rates in Solid Catalysts
- 11.3 Internal Mass Transfer and Reaction
- 11.4 The Thiele Modulus and Effectiveness Factor
- 11.5 External Mass Transfer
- 11.6 Liquid–Solid Mass Transfer Correlations
- 11.7 Effective Diffusivity
- 11.8 Minimizing Mass Transfer Effects
- 11.9 Evaluating True Kinetic Parameters
- 11.10 General Comments on Heterogeneous Reactions in Bioprocessing
- 11.11 Summary of Chapter 11
- Problems
- Uncited references
- References
- Suggestions for Further Reading
- Part 5: Bioreactors and Downstream Processes
- Chapter 12. Reactor Engineering
- Abstract
- 12.1 Bioreactor Design Perspective
- 12.2 Bioreactor Configurations
- 12.3 Practical Considerations for Bioreactor Construction and Operation
- 12.4 Monitoring and Control of Bioreactors
- 12.5 Ideal Reactor Operation
- 12.6 Sterilization
- 12.7 Summary of Chapter 12
- Problems
- Uncited references
- References
- Suggestions for Further Reading
- Chapter 13. Unit Operations for Downstream Processing
- Abstract
- 13.1 Overview of Downstream Processing
- 13.2 Overview of Cell Removal Operations
- 13.3 Filtration
- 13.4 Centrifugation
- 13.5 Cell Disruption
- 13.6 Aqueous Two-Phase Liquid Extraction
- 13.7 Precipitation
- 13.8 Adsorption
- 13.9 Membrane Filtration
- 13.10 Chromatography
- 13.11 Crystallization
- 13.12 Drying
- 13.13 Summary of Chapter 13
- Problems
- References
- Suggestions for Further Reading
- Part 6: Special topics and application
- Chapter 14. Special Topics
- Abstract
- 14.1 Metabolic Network Analysis for Metabolic Engineering
- 14.2 Sustainable Bioprocessing
- 14.3 Summary of Chapter 14
- Problems
- References
- Metabolic Network Analysis and Metabolic Engineering
- Appendix
- Appendix A. Conversion Factors
- Appendix B. Ideal Gas Constant
- Appendix C. Physical and Chemical Property Data
- Appendix D. Logarithms
- Index
- Edition: 3
- Published: September 27, 2024
- No. of pages (Paperback): 746
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780128221914
- eBook ISBN: 9780128221938
RC
Ross Carlson
Ross Carlson works in the Department of Chemical and Biological Engineering at Montana State University, Bozeman, MT, USA.
Affiliations and expertise
Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USAKM
Kate Morrissey
Kate Morrissey works in the Department of Chemical and Biological Engineering at Montana State University, Bozeman, MT, USA.
Affiliations and expertise
Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, USAPD
Pauline M. Doran
Pauline M. Doran
Swinburne University of Technology, Faculty of Science, Engineering and Technology, School of Science, Department of Chemistry and Biotechnology.
Professor Doran has taught bioprocess engineering and biotechnology at undergraduate and graduate levels for more than 30 years. Her most significant contributions to the field include bioreactor design and analysis for plant organ culture, foreign protein production in plant systems, and human tissue engineering using stem cells.
Affiliations and expertise
Swinburne University of Technology, Faculty of Science, Engineering and Technology, School of Science, Department of Chemistry and Biotechnology, Victoria, AustraliaRead Bioprocess Engineering Principles on ScienceDirect