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Biophysical Methods in Cell Biology
1st Edition - January 14, 2015
Editor: Ewa Paluch
Hardback ISBN:9780128011034
9 7 8 - 0 - 1 2 - 8 0 1 1 0 3 - 4
eBook ISBN:9780128013267
9 7 8 - 0 - 1 2 - 8 0 1 3 2 6 - 7
This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Chapters cover such topics as AFM, traction force microscopy,… Read more
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This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Chapters cover such topics as AFM, traction force microscopy, digital holographic microscopy, single molecule imaging, video force microscopy and 3D multicolor super-resolution screening
Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
Chapters are written by experts in the field
Cutting-edge material
Researchers and students in cell, molecular and developmental biology
Series Editors
Preface
Chapter 1. Single-molecule imaging of cytoplasmic dynein in vivo
Introduction
1. Visualization of Cytoplasmic Dynein In Vivo
2. Image Analysis
3. Conclusion
4. Methods
Chapter 2. Single-molecule imaging in live cell using gold nanoparticles
Introduction and Rationale
1. Gold Nanoparticle Synthesis and Functionalization
2. Photothermal Imaging
3. Live Cell Imaging
Conclusion
Chapter 3. Quantitative measurement of transcription dynamics in living cells
1. Visualizing Transcription in Living Cells
2. Experimental Protocols
3. Cell Segmentation
4. Measuring Spot Intensity
5. Correcting for Background MS2-GFP Level
6. Tracking Algorithms
7. Additional Cell Properties
8. Summary
Chapter 4. An easy-to-use single-molecule speckle microscopy enabling nanometer-scale flow and wide-range lifetime measurement of cellular actin filaments
Introduction
1. Methods
2. Perspectives
Chapter 5. Dissecting microtubule structures by laser ablation
Introduction
1. Theoretical Framework
2. Microtubule Organization Measurements
Discussion and Conclusion
Chapter 6. Quantifying mitochondrial content in living cells