Biophotonics and Biosensing

From Fundamental Research to Clinical Trials Through Advances of Signal and Image Processing

1st Edition - May 21, 2024
Editors: Andrea Armani, Tatevik Chalyan, David Sampson
Language: English
Paperback ISBN:
9 7 8 - 0 - 4 4 3 - 1 8 8 4 0 - 4
eBook ISBN:
9 7 8 - 0 - 4 4 3 - 1 8 8 4 1 - 1

Biophotonics and Biosensing brings together the knowledge of the basic principles of the field of light–biological tissue interaction, detection methods, data processing techni… Read more

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Biophotonics and Biosensing brings together the knowledge of the basic principles of the field of light–biological tissue interaction, detection methods, data processing techniques, and research, diagnostic, and clinical applications. It is suitable for new entrants to the field, while also highlighting the latest developments for experts. This volume includes perspectives by leading experts from the biophotonics and biosensing, biomedical engineering, and data science communities.

The book provides a basic grounding in the key theoretical principles and practical components of biophotonics and biosensing. Working principles of devices used in spectroscopy, microscopy, and optical sensing are presented, along with their application domains. The reader will learn about existing microscopy-based techniques used in biomedical applications for diagnosis and get to know different signal- and image-processing algorithms, including the state of the art in artificial intelligence approaches, as used in biophotonics. Finally, the book describes through concrete examples, including sample preparation and measurement approaches, how the field has developed, thanks to the integration of biophotonics and optical biosensing with advanced signal and image-processing.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Preface
Part One: Background and principles of biophotonics and optical biosensing
1: Tissue optics
Abstract
1.1. Introduction
1.2. Optical properties of tissue
1.3. Light-tissue interactions
1.4. Modeling of light tissue interactions
1.5. Conclusion
References
2: Optical biosensors: from working principles to detection methods of label-free devices
Abstract
2.1. Biosensors
2.2. Label-free optical biosensors
2.3. Label-free biosensing principle
2.4. Bioreceptors and bioconjugation strategies
2.5. Conclusions
References
Part Two: Microscopy techniques and optical biosensing in research, laboratory, and clinical applications
3: Fluorescence microscopy: backbone of modern biomedical research
Abstract
3.1. Fluorescence phenomena: photons in, photons out
3.2. Photodamage and the pyramid of tradeoffs
3.3. News from the coverslip: diagnostics, sequencing, and biotech engineering
3.4. What's the point? Confocal and laser-scanning imaging
3.5. Promise of light-sheet microscopy: from single molecule to whole brain
3.6. Look closer: super-resolution imaging
3.7. Going in vivo with mouse brain imaging
3.8. From ground up: building your own microscope
3.9. Conclusions
References
4: Raman spectroscopy—research lab analytics
Abstract
4.1. Introduction
4.2. Raman theory
4.3. Raman spectroscopy and microscopy instrumentation and applications
4.4. Summary
References
5: Subwavelength periodic dielectric nanostructures for biochemical sensing
Abstract
5.1. Introduction
5.2. Refractometric (refractive index) sensing
5.3. Fluorescence sensing
5.4. Chiral molecule detection by circular dichroism (CD) spectroscopy
5.5. Conclusion
References
6: Integrated photonic and plasmonic biosensors
Abstract
6.1. Introduction
6.2. Biosensor surface functionalization
6.3. Microfluidics
6.4. Biosensor performance metrics
6.5. Photonic biosensors
6.6. Plasmonic biosensors
6.7. Conclusions
References
7: Optical fiber-based biosensing: applications in biology and medicine
Abstract
7.1. Introduction
7.2. Principles of fiber optic sensors
7.3. Fiber-based biosensing techniques
7.4. Summary and outlook
References
8: Photonic biosensing at the point-of-care
Abstract
8.1. Introduction: the need for PoC biosensing
8.2. State-of-the-art commercial PoC systems
8.3. From the state-of-the-art to new frontiers in PoC diagnostics
8.4. Next generation optical and photonic PoC biosensing
8.5. Conclusions and prospective
References
9: Endomicroscopy
Abstract
9.1. Technology
9.2. Challenges and needs
9.3. Application domains
9.4. Conclusions
References
10: Optical coherence tomography technology in clinical applications
Abstract
10.1. Introduction
10.2. Theoretical background
10.3. Clinical applications of OCT
10.4. Conclusion
References
Part Three: Advanced signal/image processing and data analysis methods for microscopy and sensing techniques
11: Innovations in signal/image processing and data analysis in optical microscopy
Abstract
11.1. Introduction
11.2. Denoising
11.3. Resolution enhancement
11.4. Stitching & registration
11.5. Qualitative enhancement by color matching and stain normalization
11.6. Semantic understanding / information extraction
11.7. Challenges, limitations, and ethical considerations
11.8. Conclusion
Acknowledgments
Author contributions statement
Conflict of interest
Glossary or nomenclature list
References
12: Recent innovations in signal and image processing and data analysis in Raman spectroscopy
Abstract
12.1. Introduction
12.2. Signal processing for spectral analysis
12.3. Image processing
12.4. Conclusion
References
13: AI-driven innovations in signal/image processing and data analysis for optical coherence tomography in clinical applications
Abstract
Preface
Glossary
13.1. Introduction
13.2. OCT image enhancement
13.3. OCT image segmentation
13.4. AI OCT-based methods for screening, decision-making, and outcome prediction for disease
13.5. Challenges, opportunities, and future directions
13.6. Conclusions
References
Index

Andrea Armani

Andrea Armani is jointly appointed at the Ellison Institute of Technology as the Senior Director of Physical Sciences and Engineering and at the University of Southern California as the Ray Irani Chair in Chemical Engineering and Materials Science. The overarching mission of her research is to develop nonlinear materials and integrated devices to advance microscopy and diagnostics. She is a Fellow of Optica, SPIE, NAI, and AAAS, and the impact of her work has been recognized by the World Economic Forum and others.

Affiliations and expertise

Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA, United States

Tatevik Chalyan

Tatevik Chalyan is a postdoctoral researcher in the Brussels Photonics Team at the Vrije Universiteit Brussel, Belgium. Her research interests cover the fields of biophotonics and optical biosensing. Her work focuses on the nanofabrication and development of plasmonic substrates for surface-enhanced Raman spectroscopy and fluorescence microscopy, as well as the study of microring resonators and interferometer-based biosensors for refractive index sensing, optofluidic design, and validation. Moreover, she is an Optica Ambassador.

Affiliations and expertise

Brussels Photonics (B-PHOT), Department of Applied Physics and Photonics, Vrije Universiteit Brussel, Brussels, Belgium

David Sampson

David Sampson is Emeritus Professor at the University of Surrey and based in Perth, Western Australia. His overarching research interests are in the science and applications of light in medicine and biology, biomedical optics, and biophotonics. He is an authority in optical coherence tomography (OCT), with main interests in the microscope-in-a-needle (which targets surgical and biopsy guidance, for which he was awarded the IEEE Distinguished Lecturer Award and several other prizes), optical elastography (the micro-scale imaging of the mechanical properties of tissue), polarization-sensitive OCT and OCT angiography. His main application domains have been breast cancer, respiratory disease, and skin cancer. He is a fellow of AIMBE, IEEE, Optica, and SPIE.

Affiliations and expertise

Surrey Biophotonics, School of Physics, School of Biosciences and Medicine, University of Surrey, Guildford, Surrey