Biomolecular Interactions Part B
- 1st Edition, Volume 169 - May 12, 2022
- Imprint: Academic Press
- Editor: Arun K. Shukla
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 3 3 5 3 - 5
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 3 3 5 4 - 2
Biomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting in… Read more
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Request a sales quoteBiomolecular Interactions: Part A, Volume 169, the latest release in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Emerging Mechanisms of Targeted Protein Degradation by Molecular Glues, Design and use of programmable DNA Hydrogels, Oligomerization of membrane receptors: Approaches to measure in live cells, Interactions of alpha-synuclein with biomolecules, Gel-electrophoresis based method for biomolecular interaction, Recombinant centrosome expression in bacterial system, Reconstituting CCL5-CCR5 complex for structural and mechanistic analysis, Protein engineering and design in ion channel receptors, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Cell Biology series
- Updated release includes the latest information on biomolecular interactions instead of protein-protein interactions
Academic, government and industrial sectors
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Preface
- Chapter 1: Emerging mechanisms of targeted protein degradation by molecular glues
- Abstract
- 1: Introduction
- 2: Immunomodulatory imide drugs (IMiDs)
- 3: Splicing inhibitor sulfonamides (SPLAMs)
- 4: CDK12/13:DDB1 glues trigger cyclin K degradation
- 5: BCL6 glues induce polymerization and degradation of BCL6
- 6: Methods for measuring protein degradation
- 7: Bridging the gap from “serendegrity” to systematic screening
- 8: Protein degrader discovery by comparative drug profiling in hyponeddylated cell lines
- 9: Leveraging cell fate control elements for positive selection of protein degraders
- 10: Conclusions
- References
- Chapter 2: Visualizing G protein-coupled receptor homomers using photoactivatable dye localization microscopy
- Abstract
- 1: Introduction
- 2: Materials
- 3: A step-by-step guide to PD-PALM imaging and analysis
- 4: Future directions
- Acknowledgments
- References
- Chapter 3: Interaction of alpha-synuclein with lipids
- Abstract
- 1: Introduction
- 2: Interaction of α-syn with lipid membranes
- 3: Factors modulating α-syn membrane binding
- 4: α-syn interaction with specific membranes and membrane regions
- 5: Membrane remodeling by α-syn
- 6: Conclusion
- References
- Chapter 4: Gel-electrophoresis based method for biomolecular interaction
- Abstract
- 1: Introduction
- 2: Difference between SDS-PAGE, BN-PAGE and native gel electrophoresis
- 3: Materials
- 4: Methods
- 5: Examples
- 6: Concluding remarks
- 7: Notes
- References
- Chapter 5: Yeast 2-hybrid assay for investigating the interaction between the centrosome proteins PLK4 and STIL
- Abstract
- 1: Introduction
- 2: Material
- 3: Protocol
- 4: Results
- 5: Discussion
- 6: Troubleshooting
- References
- Chapter 6: Preparation of a stable CCL5·CCR5·Gi signaling complex for Cryo-EM analysis
- Abstract
- 1: Introduction
- 2: Methods
- 3: Discussion and conclusion
- References
- Chapter 7: Protein engineering and design in ion channels and receptors
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Receptor engineering and design
- 5: Concluding remarks
- References
- Chapter 8: Discovery of Virus-Host interactions using bioinformatic tools
- Abstract
- 1: Introduction to Virus-Host interactions
- 2: Virus-Host databases
- 3: Omics data
- 4: Artificial intelligence approaches
- 5: Virus-Host interaction network approaches
- 6: Virus-Host interaction structural prediction
- 7: Conclusions
- References
- Chapter 9: Best practices for resilient automation of biomolecular simulations
- Abstract
- 1: Introduction
- 2: Common problems and approaches to solving them
- 3: Conclusion
- 4: Script to generate next equilibrium MD simulation: “next_namd_prod.py”
- 5: Script to facilitate automated resilient FEP calculations using NAMD
- 6: Sample analysis script
- References
- Chapter 10: Label-free impedance measurements to unravel biomolecular interactions involved in G protein-coupled receptor signaling
- Abstract
- 1: Introduction
- 2: Electrochemical impedance measurements to study ligand-receptor interactions
- 3: Agonist and antagonist assays
- 4: Dose-response studies
- 5: In-depth analysis of the signal transduction pathway
- 6: Receptor desensitization
- 7: Further experimental options and current developments
- Acknowledgments
- References
- Chapter 11: Proximity labeling for investigating protein-protein interactions
- Abstract
- 1: Introduction
- 2: Nonproximity labeling based methods for studying protein-protein interactions
- 3: Proximity labeling methods for studying protein-protein interactions
- 4: Coupling mass spectrometry to proximity labeling
- 5: Advantages and disadvantages of the different methods
- 6: Summary
- References
- Chapter 12: An intrabody sensor to monitor conformational activation of β-arrestins
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Concluding remarks
- Acknowledgment
- References
- Chapter 13: Nanoluciferase-based methods to monitor activation, modulation and trafficking of atypical chemokine receptors
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Controls and additional considerations
- 5: Concluding remarks
- 6: Notes
- Acknowledgments
- References
- Chapter 14: In-cellulo chemical cross-linking to visualize protein-protein interactions
- Abstract
- 1: Introduction
- 2: Materials and methods
- 3: Troubleshooting
- 4: Additional considerations
- 5: Concluding remarks
- Acknowledgment
- References
- Chapter 15: Nanoluciferase-based complementation assay for systematic profiling of GPCR–GRK interactions
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes
- Acknowledgments
- References
- Chapter 16: DNA hydrogels: Principles, synthesis, characterization and applications to cell biology
- Abstract
- 1: Introduction
- 2: Principle
- 3: Synthesis methods
- 4: Characterization
- 5: Applications
- 6: Conclusions and future directions
- Acknowledgments
- References
- Edition: 1
- Volume: 169
- Published: May 12, 2022
- No. of pages (Hardback): 364
- No. of pages (eBook): 364
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780128233535
- eBook ISBN: 9780128233542
AS
Arun K. Shukla
Dr. Arun K. Shukla is a world leader in the field of GPCR biology and he is currently a Professor in the Department of Biological Sciences and Bioengineering at the Institute of Technology, Kanpur in India. Dr. Shukla’s research program is focused on understanding the structure, function and regulation of G protein-coupled receptors with a long-term of designing novel therapeutics with minimized side-effects.
Affiliations and expertise
Indian Institute of Technology, Kanpur, IndiaRead Biomolecular Interactions Part B on ScienceDirect