Biomedical Optical Phase Microscopy and Nanoscopy
- 1st Edition - November 5, 2012
- Latest edition
- Editors: Natan T. Shaked, Zeev Zalevsky, Lisa L Satterwhite
- Language: English
Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including… Read more
Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy
The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.
The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications.
- Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications
- Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy
- Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples
Biomedical engineers and researchers, Nanotechnology engineers and researchers, Optical engineers and researchers, Microscopy engineers
Preface
Part 1: Phase Contrast Microscopy and Differential Interference Contrast Microscopy
Chapter 1. Phase Contrast Microscopy
1.1 Introduction
1.2 How Phase Contrast Works
1.3 How to Acquire Phase Contrast Images
1.4 Experimental Uses
1.5 Limitations of Phase Optics
References
Further Reading
Chapter 2. Differential Interference Microscopy
2.1 Introduction
2.2 Measuring Shear Angle of DIC Prism
2.3 Bias Optimization
2.4 A Quantitative OI-DIC Microscope with Fast Modulation of Bias and Shear Direction
2.5 Combination of OI-DIC and Orientation-Independent Polarization Imaging
2.6 Combination of OI-DIC and Fluorescence Imaging
Acknowledgment
References
Chapter 3. Long-Term Recordings of Live Human Cells Using Phase Contrast Microscopy
3.1 Introduction
3.2 The Microscope
3.3 Correlative Live Cell–Fixed Cell Observations
References
Further Reading
Chapter 4. Phase Imaging in Plant Cells and Tissues
4.1 Out of Focus Imaging
4.2 Dark Field
4.3 Phase Contrast Techniques
4.4 Becke Line in Optical Microscopy
4.5 DIC Microscopy
4.6 Hoffmann Modulation Contrast
4.7 Adaptive Optics Microscopy
4.8 Interferometric Microscopy
4.9 Second Harmonic Imaging Microscopy
4.10 Magnetic Resonance Imaging of Plants Showing the Flow Component
Acknowledgments
References
Part 2: Digital Holographic Phase Microscopy
Chapter 5. Digital Holographic Microscopy for Measuring Biophysical Parameters of Living Cells
5.1 Introduction
5.2 Technical Introduction
5.3 Biological Applications
Acknowledgments
References
Chapter 6. Holographic Microscopy Techniques for Multifocus Phase Imaging of Living Cells
6.1 Introduction
6.2 DHM Setups for Live Cell Imaging
6.3 Recording and Numerical Evaluation of Digital Holograms
6.4 Refractive Index Determination of Cells in Suspension
6.5 DHM Analysis of Morphology and Thickness Changes
6.6 Quantitative Cell Division Monitoring
6.7 Cell Tracking in 3D Environments
6.8 Conclusions
Acknowledgments
References
Chapter 7. Phase Unwrapping Problems in Digital Holographic Microscopy of Biological Cells
7.1 Introduction
7.2 Experimental Techniques
7.3 Varying Reconstruction Distance Method
7.4 Dual-Wavelength Phase Imaging
7.5 Conclusions
References
Chapter 8. On-Chip Holographic Microscopy and its Application for Automated Semen Analysis
8.1 Introduction
8.2 Partially Coherent In-Line Holography on a Chip
8.3 Reconstruction of Microscopic Images and the Phase Recovery Process
8.4 On-Chip Imaging of Sperm Using Partially Coherent Lensfree In-Line Holography
8.5 High-Throughput On-Chip Semen Analysis Results
8.6 Conclusions
Acknowledgment
References
Chapter 9. Synthetic Aperture Lensless Digital Holographic Microscopy (SALDHM) for Superresolved Biological Imaging
9.1 Introduction
9.2 SALDHM Inside the Gabor’s Regime
9.3 SALDHM Outside the Gabor’s Regime
9.4 Conclusions
References
Chapter 10. Combining Digital Holographic Microscopy with Microfluidics: A New Tool in Biotechnology
10.1 Introduction
10.2 Drive and Analyze
10.3 Particle Tracking in 3D
10.4 Conclusions
References
Chapter 11. Holographic Motility Contrast Imaging of Live Tissues
11.1 Introduction and Review
11.2 Optical Coherence Imaging
11.3 Motility Contrast Imaging
11.4 Dynamic Light Scattering (DLS) Spectroscopy
11.5 Tissue Dynamics Spectroscopy
Acknowledgment
References
Part 3: Advanced Interferometric and Polarization Techniques
Chapter 12. Tomographic Phase Microscopy (TPM)
12.1 Introduction
12.2 Theory—Optical Diffraction Tomography
12.3 Experimental Implementation
12.4 Video-Rate TPM
12.5 Biological Applications
12.6 Conclusion
References
Chapter 13. Phase-Sensitive Optical Coherence Microscopy (OCM)
13.1 Introduction
13.2 OCM Principles
13.3 Phase OCM
13.4 Phase OCM Applications
13.5 Conclusions
References
Chapter 14. Polarization and Spectral Interferometric Techniques for Quantitative Phase Microscopy
14.1 Introduction
14.2 Dual-Interference Channel Quantitative Phase Microscopy
14.3 Spectral-Domain Differential Interference Contrast Microscopy
14.4 Spectral Multiplexing by RGB Color Channels
14.5 Nonlinear Phase Dispersion Spectroscopy
14.6 Conclusions
Acknowledgments
References
Chapter 15. Polarization Microscopy
15.1 Introduction
15.2 Traditional Polarized Light Microscopy
15.3 The Liquid-Crystal Polarization Microscope (LC-PolScope)
15.4 Practical Considerations
15.5 Polarized Light Imaging in Three Dimensions
15.6 Conclusion
15.7 Glossary of Polarization Optical Terms
Acknowledgments
References
Part 4: Phase Nanoscopy
Chapter 16. Is There a Fundamental Limit to Spatial Resolution in Phase Microscopy?
16.1 Introduction
16.2 Where Was Abbe’s Theory Incomplete?
16.3 Parallel Full-Field Linear Imaging
16.4 Reconstruction from Diffraction Patterns
16.5 Structured Illumination Microscopy
16.6 Imaging Three-Dimensional Phase Objects
16.7 Conclusions
Acknowledgments
References
Chapter 17. Nano-Holographic Interferometry for In-Vivo Observations
17.1 Introduction: Theoretical Basis of Nano Fourier Transform Holography
17.2 Gratings Illuminated by Evanescent Waves
17.3 Basic Setup Utilized for the Observation of Nano-Objects
17.4 System of Fringes Contained in the Recorded Image: Multi-k Vector Fields
17.5 Determination of the Pitch of the Gratings
17.6 Formation of the Holograms at the Nanoscale
17.7 Procedures to Extract Metrological Information from the Recorded Images
17.8 Analysis of the Scales
17.9 Observation of Nano-Sized Objects
17.10 Conclusions
References
Chapter 18. Fluorescence Phase Microscopy (FPM) and Nanoscopy
18.1 Introduction
18.2 The Fluorescence Self-Interference Process
18.3 Experimental Setups of FPM
18.4 Applications of FPM
18.5 Conclusions and Outlook
References
Index
- Edition: 1
- Latest edition
- Published: November 5, 2012
- Language: English
NS
Natan T. Shaked
Affiliations and expertise
Senior Lecturer, Department of Biomedical Engineering, Tel Aviv UniversityZZ
Zeev Zalevsky
Affiliations and expertise
Faculty of Engineering and the Center for Advanced Materials and Nanotechnology, Bar-Ilan UniversityLS
Lisa L Satterwhite
Affiliations and expertise
Duke University, DurhamRead Biomedical Optical Phase Microscopy and Nanoscopy on ScienceDirect