Biological Events Probed by Ultrafast Laser Spectroscopy
- 1st Edition - January 28, 1982
- Imprint: Academic Press
- Editor: Robert R. Alfano
- Language: English
- Paperback ISBN:9 7 8 - 0 - 1 2 - 4 1 4 6 3 9 - 6
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 1 4 9 5 8 - 7
Biological Events Probed by Ultrafast Laser Spectroscopy summarizes the progress in the experimental and theoretical understanding of primary phenomena occurring in biology on a… Read more
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Request a sales quoteBiological Events Probed by Ultrafast Laser Spectroscopy summarizes the progress in the experimental and theoretical understanding of primary phenomena occurring in biology on a picoseconds and nanosecond time scale. This book includes basic principles, survey of research results, and thinking of experts in the fields of photosynthesis, vision, hemoglobin, and DNA. This reference is organized into five parts. The primary events in the various areas of biology are reviewed in the first four parts. The last part covers the picosecond and subpicosecond laser techniques. Primary processes in photosynthesis are then discussed. This is followed by chapters on fluorescence and absorption kinetic measurements in higher plants. Subsequent chapters cover kinetic measurements in bacteria photosynthesis, theories of primary energy transfer appropriate to photosynthesis, and multiexcitation processes. Part II examines primary visual processes, including kinetic experimental work on rhodopsin and bacteriorhodopsin, and theoretical concepts on primary events in vision. The third part presents the introductory materials on the kinetic models of hemoglobin and myoglobin. It also discusses the interesting picosecond measurements on these proteins. Part IV focuses on the application of modern ultrafast temporal techniques to the study of DNA and its components, as well as DNA-dye complexes. The concluding part introduces the principles of design and operation of the solid state laser and picoseconds spectroscopy methods. A discussion on pulse generation methods and measurement techniques applicable for measuring primary events in biological systems is provided. This book is useful both for the newbies and experts in the field of ultrafast phenomena. It aims to attract biologists, chemists, physicists, and engineers who are interested in biological processes. It will help readers find all the necessary and relevant material in one presentation.
List of ContributorsPrefacePart I Photosynthesis Chapter 1 Primary Processes of Oxygen-Evolving Photosynthesis I. Introduction II. The Photosynthetic Apparatus III. Fundamental Concepts of Photosynthesis IV. The Primary Events V. Conclusion References Chapter 2 Time-Resolved Fluorescence Spectroscopy I. Introduction II. Time-Resolved Fluorescence Spectroscopy Techniques III. Fluorescence Kinetic Measurements in Photosynthesis IV. Future Directions References Chapter 3 Early Photochemical Events in Green Plant Photosynthesis: Absorption and EPR Spectroscopic Studies I. Introduction II. The Primary Electron Donors of the Two Photosystems III. Early Photochemical Events in the Electron Acceptor Systems of Photosystems I and II References Chapter 4 Electron Transfer Reactions in Reaction Centers of Photosynthetic Bacteria and in Reaction Center Models I. In Vivo Electron Transfer Reaction II. In Vitro Electron Transfer Reactions References Chapter 5 Photoprocesses in Chlorophyll Model Systems I. Introduction II. Chlorophyll and the Photosynthetic Unit III. Chlorophyll Properties Relevant to Models IV. Covalently Linked Pairs as Photoreaction Center Models V. Energy Transfer in Self-Assembled Systems VI. Energy Transfer in the ChlsP Antenna Model VII. A Model for Electron Transfer from the Primary Donor References Chapter 6 Exciton Annihilation and Other Nonlinear High-Intensity Excitation Effects I. Introduction II. Photophysical Phenomena III. Laser Characteristics in Relation to the Different Photophysical Processes IV. Single Picosecond Pulse Excitation V. Nanosecond Pulse Excitation VI. Microsecond Pulse Excitation VII. Picosecond Pulse Excitation Studies on Absorption Changes in Bacterial Reaction Centers References Chapter 7 Fluorescence Decay Kinetics and Bimolecular Processes in Photosynthetic Membranes I. Introduction II. Fluorescence Decay Profiles: Low-Intensity Regime III. Exciton-Exciton Annihilation: High Excitation Intensities IV. Conclusion References Chapter 8 Statistical Theory of the Effect of Multiple Excitation in Photosynthetic Systems I. Introduction II. Statistics III. Kinetics IV. Application V Comparison with Other Treatments VI. Conclusions Appendix I: Calculation of Yield with Escape at Traps Appendix II References Part II VISION Chapter 9 An Introduction to Visual Pigments and Purple Membranes and Their Primary Processes I. Introduction II. The Free Chromophore III. Chromophore Binding and Color IV. Light and Dark Reactions V. The Primary Photochemical Event References Chapter 10 Dynamics of the Primary Events in Vision I. Rhodopsin II. Isorhodopsin III. Hypsorhodopsin IV. Concluding Remarks References Chapter 11 Primary Events in Bacteriorhodopsin I. Introduction II. Absorption Measurements III. Fluorescence Measurements References Chapter 12 Theoretical Aspects of Photoisomerization in Visual Pigments and Bacteriorhodopsin I. Introduction II. Absorption Spectra III. The Primary Photochemical Event IV. Excited State Processes V. Concluding Remarks References Chapter 13 Simulation of the Primary Event in Rhodopsin Photochemistry Using Semiempirical Molecular Dynamics Theory I. Introduction II. Theoretical Approaches in the Simulation of Intramolecular Dynamics III. Semiempirical Molecular Dynamics Theory and Rhodopsin Photochemistry IV. Comments and Conclusions References Part III HEMOPROTEINS Chapter 14 Introduction to Hemoproteins I. The Importance of Hemoprotein Studies II. Structure and States III. Fast Elementary Processes IV. The Individual Steps References Chapter 15 The Study of the Primary Events in the Photolysis of Hemoglobin and Myoglobin Using Picosecond Spectroscopy I. Introduction II. Review of Binding and Photodissociation of Molecular Oxygen and Carbon Monoxide in Heme Compounds III. Experimental Results IV. Picosecond Photodissociation Experiments, Related Experiments, and Results V. Conclusions ReferencesPart IV DNA Chapter 16 Ultrafast Techniques Applied to DNA Studies Stanley L. Shapiro I. Introduction II. Nonlinear Optical Effects and Selective Action—Proposed Infrared Schemes III. Photochemical Reactions in Nucleic Acid Components Induced by Visible and Ultraviolet Pulses IV. Selectivity in a Mixture of Bases and in More Complex Nucleic Acid Components V. Selectivity in a RNA Component and Multiphoton Experiments with Viruses and Plasmids VI. Theory of Selectivity on Nucleic Acid Components VII. Selective Photodamage of Dye-Biomolecule Complexes VIII. Picosecond Depolarization Measurements and Torsional Motions in DNA References Part V ULTRAFAST LASER TECHNIQUES Chapter 17 Picosecond Laser Techniques and Design I. Introduction II. General Principles of Operation of Picosecond Lasers III. Laser Components and Designs IV. Single-Pulse Selection and Amplification V. Obtaining the Right Wavelength VI. Time Measurements and Applications References Chapter 18 Subpicosecond Ultrafast Laser Technique—Application and Design I. Introduction II. Subpicosecond Pulse Generation III. Measurement Techniques IV. Future Directions ReferencesIndex
- Edition: 1
- Published: January 28, 1982
- No. of pages (eBook): 458
- Imprint: Academic Press
- Language: English
- Paperback ISBN: 9780124146396
- eBook ISBN: 9780323149587
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Robert R. Alfano
Robert A. Alfano is Distinguished Professor and Fellow at The City College of City University of New York, USA. He is also an Optical Society of America; Fellow, and a Fellow of IEEE. He has been involved in developing ultrafast laser spectroscopic techniques and applications of these techniques to study ultrafast dynamical processes in physical, chemical, and biological systems. His research encompasses the study and development of supercontinuum, tunable solid-state lasers, nonlinear optical processes, application of optical spectroscopic techniques for medical diagnosis (optical biopsy), study of photon migration in turbid media, and development of optical imaging techniques for biomedical imaging (optical mammography). He has published more than 700 papers and holds 102 patents.
Affiliations and expertise
Professor and Fellow, The City College of City University of New York, USARead Biological Events Probed by Ultrafast Laser Spectroscopy on ScienceDirect