Biofilms
- 1st Edition, Volume 53 - July 11, 2023
- Imprint: Academic Press
- Editor: Volker Gurtler
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 8 8 3 3 - 9
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 8 8 3 4 - 6
Biofilms, Volume 53 in the ongoing Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of ti… Read more
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Request a sales quoteBiofilms, Volume 53 in the ongoing Methods in Microbiology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including Monospecies and polymicrobial biofilms in static and flow environment, Methods used to study biofilms, Spatial analysis of bacterial biofilms, Biofilm grown in bioreactors, Single-cell analysis of subpopulations within biofilms using microscopy, flow cytometry, and imaging flow cytometry, Microscopy analysis of biofilm-mineral interactions or mineral formation within biofilms, Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions, Studying Gene Expression in Biofilms, and more.
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Undergraduates, graduates, academics, and researchers in the field of microbiology
- Cover image
- Title page
- Table of Contents
- Series Page
- Copyright
- Contributors
- Preface
- References
- Section 1: Plant biofilms
- Chapter 1: Bacterial biofilms as an essential component of rhizosphere plant-microbe interactions
- Abstract
- 1: Introduction
- 2: Rhizosphere—A microbial habitat shaped by root exudates
- 3: Experimental approaches for studying rhizosphere biofilms
- 4: Sensing the plant host: Bacterial chemotaxis towards plant roots exudates
- 5: Colonizing the plant root
- 6: The importance of rhizosphere biofilms
- 7: Conclusions
- Acknowledgements
- References
- Section 2: Mixed species
- Chapter 2: Monospecies and polymicrobial biofilms in static and flow environment
- Abstract
- 1: Introduction
- 2: Biofilm and flow environment
- 3: Key resources table
- 4: Materials and equipment
- 5: Step-by-step method details
- 6: Quantification and statistical analysis
- 7: Optimization and troubleshooting
- 8: Safety considerations and standards
- 9: Alternative methods/procedures
- References
- Further reading
- Chapter 3: Optimization of protocol for quantification of biofilm formed by pathogenic rapidly-growing nontuberculous mycobacteria for diagnostic screening
- Abstract
- 1: Introduction
- 2: Biofilm culture of the NTM strain
- 3: Crystal violet assay of the NTM biofilm
- 4: Response surface methodology based optimization of culture conditions favouring maximum biofilm formation by NTM
- 5: Sonication and colony forming unit (CFU) count of NTM biofilm
- 6: Determination of the number of days required for NTM biofilm formation
- 7: Basic fuchsin staining of biofilm-forming cells of the pathogenic NTM strain
- 8: Scanning electron microscopy (SEM) of NTM planktonic and biofilm-forming cells
- 9: Conclusion
- Acknowledgement
- References
- Chapter 4: Investigating inter-kingdom interaction between oral Streptococcus mutans and Candida species in mixed-species biofilms
- Abstract
- 1: Introduction
- 2: Materials and methods
- 3: Results and discussions
- 4: Conclusion
- References
- Section 3: Nano techniques
- Chapter 5: Programming bacterial adhesion to functionalized surfaces through cellular display of recombinant nanobodies
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Notes/troubleshooting
- Acknowledgements
- References
- Chapter 6: Micro- and nanoscale techniques for studying biofilm-mineral interactions
- Abstract
- 1: Introduction
- 2: Confocal laser scanning microscopy (CLSM) to image biofilm-minerals assemblages in situ, at the submicrometer scale
- 3: Optical coherence tomography (OCT) to in situ characterize the structure of thick biofilms at the micrometre scale over large areas
- 4: Atomic force microscopy (AFM) to image the topography, and mechanical, electrical and chemical properties of biofilm-mineral assemblages at the sub-nanometre scale
- 5: Vertical scanning interferometry (VSI) to quantify dissolution rates of mineral substrates covered by biofilms
- 6: Analyses of biofilm-mineral interactions down to the nanometre scale by scanning electron microscopy and helium ion microscopy
- 7: Focused ion beam milling for TEM sample preparation and 3D nanoscale investigation of biofilm-mineral interactions
- 8: Transmission electron microscopy (TEM) to study biofilm-mineral interactions
- 9: Characterizing chemical element distribution and speciation in biofilm-mineral assemblages by spectroscopies
- Acknowledgements
- References
- Section 4: Genetics and microfluidics
- Chapter 7: Methods for studying biofilms: Microfluidics and translation in the clinical context
- Abstract
- 1: Introduction
- 2: General in vitro methods to study biofilms
- 3: Microfluidics for biofilm study
- 4: From bench to bedside
- Acknowledgements
- Author contribution
- References
- Chapter 8: Studying gene expression in biofilms
- Abstract
- 1: Why study gene expression of biofilm cells?
- 2: Methods for studying individual genes
- 3: Methods for studying transcriptomes
- 4: Methods for studying heterogeneity of gene expression
- 5: Conclusions
- Acknowledgement
- References
- Section 5: Microsocopy
- Chapter 9: Spatial analysis of multispecies bacterial biofilms
- Abstract
- 1: Introduction
- 2: General workflow for biofilms spatial analysis
- 3: Experimental protocol
- Acknowledgements
- References
- Chapter 10: Use of epifluorescence widefield deconvolution microscopy for imaging and three-dimensional rendering of Pseudomonas aeruginosa biofilms and extracellular matrix materials
- Abstract
- 1: Introduction
- 2: Materials and methods
- 3: Results and discussion
- 4: Conclusions
- Acknowledgements
- References
- Edition: 1
- Volume: 53
- Published: July 11, 2023
- No. of pages (Hardback): 210
- No. of pages (eBook): 210
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780323988339
- eBook ISBN: 9780323988346
VG
Volker Gurtler
Dr Gurtler is a Molecular biologist with extensive industry experience and expertise in DNA analysis for the identification and typing of organisms. He is known internationally as the author of the original methodology of ribotyping. He is Advisor to Geneious (Bioinformatics software company) and previous head of Molecular Biology at Austin Health Microbiology, currently Adjunct Professor at RMIT University, Editor of Journal of Microbiological Methods, Editor of Heliyon and Series Editor of Methods in Microbiology. Dr Gurtler is currently working in collaboration with Assoc. Prof Danilla Grando on ribosome analysis of E. coli isolates and association with virulence. He visits RMIT regularly to advise on experimental methods and help PhD students use Geneious Bioinformatic software.
Affiliations and expertise
Adjunct Professor, RMIT University, Melbourne, AustraliaRead Biofilms on ScienceDirect