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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce… Read more
LIMITED OFFER
Immediately download your ebook while waiting for your print delivery. No promo code needed.
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science.
Biomedical researchers; residents and medical students; clinical researchers and students in various medical areas as well as pharmaceutical science and bioengineering
Chapter 1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR
Chapter 2. Methods of Cloning
Chapter 3. Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos
Chapter 4. Microarrays: An Introduction and Guide to Their Use
Chapter 5. Analysis of Human Genetic Variations Using DNA Sequencing
Chapter 6. Western Blot
Chapter 7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research
Chapter 8. Immunofluorescence
Chapter 9. Cell Culture: Growing Cells as Model Systems In Vitro
Chapter 10. Flow Cytometry
Chapter 11. Transfection
Chapter 12. In Vivo Animal Modeling: Drosophila
Chapter 13. Zebrafish as a Research Organism: Danio rerio in Biomedical Research
Chapter 14. Xenopus as a Model Organism for Biomedical Research
Chapter 15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research
Appendix A. Legal Framework on the Scientific Use of Animals in Research
Appendix B. Regulatory Frameworks for Stem Cell Research
Appendix C. Using Multiple Experimental Methods to Address Basic Science Research Questions
MJ
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MJ