Bailey & Scott's Diagnostic Microbiology
- 14th Edition - December 28, 2015
- Imprint: Mosby
- Author: Patricia M. Tille
- Language: English
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 4 2 8 9 2 - 7
Perfect your lab skills with the gold standard in microbiology! Serving as both the #1 bench reference for practicing microbiologists and as a favorite text for students in cl… Read more
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Request a sales quotePerfect your lab skills with the gold standard in microbiology! Serving as both the #1 bench reference for practicing microbiologists and as a favorite text for students in clinical laboratory science programs, Bailey & Scott’s Diagnostic Microbiology, 14th Edition covers all the topical information and critical thinking practice you need for effective laboratory testing. This new edition also features hundreds step-by-step procedures, updated visuals, new case studies, and new material on the latest trends and equipment in clinical microbiology — including automation, automated streaking, MALDI-TOF, and incubator microscopes. It’s everything you need to get quality lab results in class and in clinical practice!
- More than 800 detailed, full-color illustrations aid comprehension and help in visualizing concepts.
- Expanded sections on parasitology, mycology, and virology eliminate the need to purchase separate books on this material.
- General and Species boxes in the organism chapters highlight the important topics that will be discussed in the chapter.
- Case studies provide the opportunity to apply information to a variety of diagnostic scenarios, and help improve decision-making and critical thinking skills.
- Hands-on procedures include step-by-step instructions, full-color photos, and expected results.
- A glossary of terms is found at the back of the book for quick reference.
- Learning objectives begin each chapter, offering a measurable outcome to achieve by the completing the material.
- Learning resources on the Evolve companion website enhance learning with review questions and procedures.
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- Reviewers
- Contributors
- Preface
- Acknowledgments
- I. Basic Medical Microbiology
- 1. Microbial taxonomy
- Classification
- Nomenclature
- Identification
- Chapter review
- Bibliography
- 2. Bacterial genetics, metabolism, and structure
- Bacterial genetics
- Bacterial metabolism
- Structure and function of the bacterial cell
- Chapter review
- Bibliography
- 3. Host-microorganism interactions
- The encounter between host and microorganism
- Microorganism colonization of host surfaces
- Microorganism entry, invasion, and dissemination
- Outcome and prevention of infectious diseases
- Chapter review
- Bibliography
- II. General Principles in Clinical Microbiology
- Section 1. Safety and Specimen Management
- 4. Laboratory safety
- Sterilization, disinfection, and decontamination
- Chemical safety
- Fire safety
- Electrical safety
- Handling of compressed gases
- Biosafety
- Exposure control plan
- Employee education and orientation
- Disposal of hazardous waste
- Standard precautions
- Engineering controls
- Classification of biologic agents based on hazard
- Mailing biohazardous materials
- Chapter review
- Bibliography
- 5. Specimen management
- General concepts for specimen collection and handling
- Specimen preservation
- Specimen workup
- Expediting results reporting: Computerization
- Chapter review
- Bibliography
- Section 2. Approaches to Diagnosis of Infectious Diseases
- 6. Role of microscopy
- Bright-field (light) microscopy
- Phase-contrast microscopy
- Fluorescent microscopy
- Dark-field microscopy
- Electron microscopy
- Digital automated microscopy
- Chapter review
- Bibliography
- 7. Traditional cultivation and identification
- Organism identification
- Principles of bacterial cultivation
- Bacterial cultivation
- Principles of identification
- Principles of phenotypic identification schemes
- Commercial identification systems and automation
- Chapter review
- Bibliography
- 8. Nucleic acid–based analytic methods for microbial identification and characterization
- Overview of nucleic acid–based methods
- Postamplification and traditional analysis
- Chapter review
- Bibliography
- 9. Overview of immunochemical methods used for organism detection
- Features of the immune response
- Production of antibodies for use in laboratory testing
- IgM clinical significance
- Separating IgM from IgG for serologic testing
- Principles of immunochemical methods used for organism detection
- Summary
- Chapter review
- Bibliography
- Section 3. Evaluation of Antimicrobial Activity
- 10. Principles of antimicrobial action and resistance
- Antimicrobial action
- Mechanisms of antibiotic resistance
- Chapter review
- Bibliography
- 11. Laboratory methods and strategies for antimicrobial susceptibility testing
- Goal and limitations
- Testing methods
- Laboratory strategies for antimicrobial susceptibility testing
- Accuracy
- Communication
- Chapter review
- Bibliography
- Reference
- III. Bacteriology
- Section 1. Principles of Identification
- 12. Overview of bacterial identification methods and strategies
- Rationale for approaching organism identification
- Future trends of organism identification
- Bibliography
- Section 2. Catalase-Positive, Gram-Positive Cocci
- 13. Staphylococcus, micrococcus, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 3. Catalase-Negative, Gram-Positive Cocci
- 14. Streptococcus, enterococcus, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 4. Non-Branching, Catalase-Positive, Gram-Positive Bacilli
- 15. Bacillus and similar organisms
- General characteristics
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 16. Listeria, corynebacterium, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Treatment
- Chapter review
- Bibliography
- Section 5. Non-Branching, Catalase-Negative, Gram-Positive Bacilli
- 17. Erysipelothrix, lactobacillus, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 6. Branching or Partially Acid-Fast, Gram-Positive Bacilli
- 18. Nocardia, streptomyces, rhodococcus, and similar organisms
- General characteristics
- Epidemiology and pathogenesis
- Spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 7. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Negative)
- 19. Enterobacteriaceae
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of diseases
- Specific organisms
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 20. Acinetobacter, stenotrophomonas, and other organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial resistance and antimicrobial susceptibility testing
- Antimicrobial therapy
- Prevention
- Chapter review
- Bibliography
- Section 8. Gram-Negative Bacilli and Coccobacilli (MacConkey-Positive, Oxidase-Positive)
- 21. Pseudomonas, burkholderia, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 22. Achromobacter, rhizobium, ochrobactrum, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 23. Chryseobacterium, sphingobacterium, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 24. Alcaligenes, bordetella (nonpertussis), comamonas, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 25. Vibrio, aeromonas, and similar organisms
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 9. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Positive)
- 26. Sphingomonas paucimobilis and similar organisms
- General considerations
- Epidemiology, spectrum of disease, and antimicrobial therapy
- Laboratory diagnosis
- Prevention
- Chapter review
- Bibliography
- 27. Moraxella
- General characteristics
- Epidemiology, spectrum of disease, and antimicrobial therapy
- Laboratory diagnosis
- Prevention
- Chapter review
- Bibliography
- 28. Eikenella corrodens and similar organisms
- General characteristics
- Epidemiology, spectrum of disease, and antimicrobial therapy
- Laboratory diagnosis
- Serodiagnosis
- Prevention
- Chapter review
- Bibliography
- 29. Pasteurella and similar organisms
- General characteristics and taxonomy
- Epidemiology, spectrum of disease, and antimicrobial therapy
- Laboratory diagnosis
- Serodiagnosis
- Prevention
- Chapter review
- Bibliography
- 30. Actinobacillus, kingella, cardiobacterium, capnocytophaga, and similar organisms
- General characteristics
- Epidemiology, pathogenesis, and spectrum of disease and antimicrobial therapy
- Laboratory diagnosis
- Serodiagnosis
- Prevention
- Chapter review
- Bibliography
- Section 10. Gram-Negative Bacilli and Coccobacilli (MacConkey-Negative, Oxidase-Variable)
- 31. Haemophilus
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 11. Gram-Negative Bacilli that are Optimally Recovered on Special Media
- 32. Bartonella and afipia
- Bartonella
- Afipia felis
- Chapter review
- Bibliography
- 33. Campylobacter, arcobacter, and helicobacter
- Campylobacter and arcobacter
- Helicobacter spp.
- Chapter review
- Bibliography
- 34. Legionella
- General characteristics
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 35. Brucella
- General characteristics
- Epidemiology and pathogenesis
- Spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 36. Bordetella pertussis, bordetella parapertussis, and related species
- General characteristics
- Spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 37. Francisella
- General characteristics
- Epidemiology and pathogenesis
- Spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 38. Streptobacillus moniliformis and spirillum minus
- Streptobacillus moniliformis
- Spirillum minus
- Chapter review
- Bibliography
- Section 12. Gram-Negative Cocci
- 39. Neisseria and moraxella catarrhalis
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- Section 13. Anaerobic Bacteriology
- 40. Overview and general laboratory considerations
- General characteristics
- Specimen collection and transport
- Macroscopic examination of specimens
- Direct detection methods
- Specimen processing
- Anaerobic media
- Approach to identification
- Antimicrobial susceptibility testing and therapy
- Chapter review
- Bibliography
- 41. Overview of anaerobic organisms
- Epidemiology
- Pathogenesis and spectrum of disease
- Prevention
- Chapter review
- Bibliography
- Section 14. Mycobacteria and Other Bacteria with Unusual Growth Requirements
- 42. Mycobacteria
- Mycobacterium tuberculosis complex
- Nontuberculous mycobacteria
- Laboratory diagnosis of mycobacterial infections
- Antimicrobial susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 43. Obligate intracellular and nonculturable bacterial agents
- Chlamydia
- Rickettsia, orientia, anaplasma, and ehrlichia
- Coxiella
- Tropheryma whipplei
- Klebsiella granulomatis
- Chapter review
- Bibliography
- 44. Cell wall-deficient bacteria: Mycoplasma and ureaplasma
- General characteristics
- Epidemiology and pathogenesis
- Spectrum of disease
- Laboratory diagnosis
- Cultivation
- Susceptibility testing and therapy
- Prevention
- Chapter review
- Bibliography
- 45. The spirochetes
- Treponema
- Borrelia
- Brachyspira
- Leptospira
- Prevention
- Chapter review
- Bibliography
- IV. Parasitology
- 46. Overview of the methods and strategies in parasitology
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Approach to identification
- Prevention
- Chapter review
- 47. Intestinal protozoa
- Amoebae
- Flagellates
- Ciliates
- Sporozoa (apicomplexa)
- Microsporidia
- Chapter review
- Bibliography
- 48. Blood and tissue protozoa
- Plasmodium spp.
- Babesia spp.
- Trypanosoma spp.
- Leishmania spp.
- Chapter review
- Bibliography
- 49. Protozoa from other body sites
- Free-living amoebae
- Naegleria fowleri
- Acanthamoeba spp.
- Acanthamoeba keratitis
- Balamuthia mandrillaris
- Trichomonas vaginalis
- Pentatrichomonas hominis
- Toxoplasma gondii
- Chapter review
- Bibliography
- 50. Intestinal nematodes
- Ascaris lumbricoides
- Enterobius vermicularis
- Strongyloides stercoralis
- Trichostrongylus spp.
- Trichuris trichiura
- Capillaria philippinensis
- Hookworms
- Ancylostoma duodenale
- Necator americanus
- Results and reporting
- Chapter review
- Bibliography
- 51. Tissue nematodes
- Trichinella spiralis
- Toxocara canis (visceral larva migrans) and toxocara cati (ocular larva migrans)
- Ancylostoma braziliense or ancylostoma caninum (cutaneous larva migrans)
- Dracunculus medinensis
- Parastrongylus cantonensis (cerebral angiostrongyliasis)
- Parastrongylus costaricensis (abdominal angiostrongyliasis)
- Gnathostoma spinigerum
- Dirofilaria immitis and other species
- Chapter review
- Bibliography
- 52. Blood nematodes
- Wuchereria bancrofti
- Brugia malayi and brugia timori
- Loa loa
- Onchocerca volvulus
- Mansonella spp. (M. ozzardi, M. streptocerca, M. perstans)
- Chapter review
- Bibliography
- 53. Intestinal cestodes
- Diphyllobothrium latum
- Dipylidium caninum
- Hymenolepis nana
- Hymenolepis diminuta
- Taenia solium
- Taenia saginata
- Chapter review
- Bibliography
- 54. Tissue cestodes
- Taenia solium
- Echinococcus granulosus
- Echinococcus multilocularis
- Taenia multiceps and other species
- Spirometra mansonoides
- Chapter review
- Bibliography
- 55. Intestinal trematodes
- Fasciolopsis buski
- Heterophyes and metagonimus yokogawai
- Chapter review
- Bibliography
- 56. Liver and lung trematodes
- The liver flukes
- The lung flukes
- Chapter review
- Bibliography
- 57. Blood trematodes
- General characteristics
- Epidemiology
- Pathology and spectrum of disease
- Laboratory diagnosis
- Therapy
- Prevention
- Chapter review
- Bibliography
- V. Mycology
- 58. Overview of fungal identification methods and strategies
- Epidemiology
- General features of the fungi
- Taxonomy of the fungi
- Clinical classification of the fungi
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- General considerations for the identification of yeasts
- General considerations for the identification of molds
- General morphologic features of the molds
- Clinical relevance for fungal identification
- Laboratory safety
- Prevention
- Chapter review
- Bibliography
- 59. Hyaline molds, mucorales, entomophthorales, dermatophytes, and opportunistic and systemic mycoses
- The mucorales
- The entomophthorales
- The dermatophytes
- The opportunistic mycoses
- Systemic mycoses
- Chapter review
- Bibliography
- References
- 60. Dematiaceious (melanized) molds
- General characteristics
- Epidemiology and pathogenesis
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Chapter review
- Bibliography
- 61. Opportunistic atypical fungus: pneumocystis jirovecii
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Chapter review
- Bibliography
- References
- 62. The yeasts
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Laboratory diagnosis
- Commercially available yeast identification systems
- Conventional yeast identification methods
- Chapter review
- Bibliography
- Reference
- 63. Antifungal susceptibility testing, therapy, and prevention
- Antifungal susceptibility testing
- Antifungal therapy and prevention
- Chapter review
- Bibliography
- VI. Virology
- 64. Overview of the methods and strategies in virology
- General characteristics
- Epidemiology
- Pathogenesis and spectrum of disease
- Prevention and therapy
- Viruses that cause human diseases
- Laboratory diagnosis
- Chapter review
- Bibliography
- 65. Viruses in human disease
- Viruses in human disease
- Adenoviruses
- Arenaviruses
- Bunyaviruses
- Caliciviruses
- Coronaviruses
- Filoviruses
- Flaviviruses
- Hepevirus
- Hepadnaviruses
- Herpes viruses
- Orthomyxoviruses
- Papillomaviruses
- Paramyxoviruses
- Parvoviruses
- Picornaviruses
- Polyomaviruses
- Poxviruses
- Reoviruses
- Retroviruses
- Rhabdoviruses
- Togaviruses
- Miscellaneous viruses
- Interpretation of laboratory test results
- Prions in human disease
- Chapter review
- Bibliography
- 66. Antiviral therapy, susceptibility testing, and prevention
- Antiviral therapy
- Antiviral resistance
- Methods of antiviral susceptibility testing
- Prevention of other viral infections
- Chapter review
- Bibliography
- VII. Diagnosis by Organ System
- 67. Bloodstream infections
- General considerations
- Detection of bacteremia
- Special considerations for other relevant organisms isolated from blood
- Chapter review
- Bibliography
- 68. Infections of the lower respiratory tract
- General considerations
- Diseases of the lower respiratory tract
- Laboratory diagnosis of lower respiratory tract infections
- Chapter review
- Bibliography
- 69. Upper respiratory tract infections and other infections of the oral cavity and neck
- General considerations
- Diseases of the upper respiratory tract, oral cavity, and neck
- Diagnosis of upper respiratory tract infections
- Diagnosis of infections in the oral cavity and neck
- Chapter review
- Bibliography
- 70. Meningitis and other infections of the central nervous system
- General considerations
- Shunt infections
- Laboratory diagnosis of central nervous system infections
- Chapter review
- Bibliography
- 71. Infections of the eyes, ears, and sinuses
- Eyes
- Ears
- Sinuses
- Chapter review
- Bibliography
- 72. Infections of the urinary tract
- General considerations
- Infections of the urinary tract
- Laboratory diagnosis of urinary tract infections
- Chapter review
- Bibliography
- 73. Genital tract infections
- General considerations
- Genital tract infections
- Laboratory diagnosis of genital tract infections
- Chapter review
- Bibliography
- 74. Gastrointestinal tract infections
- Anatomy
- Resident gastrointestinal microbiome
- Gastroenteritis
- Other infections of the gastrointestinal tract
- Laboratory diagnosis of gastrointestinal tract infections
- Chapter review
- Bibliography
- 75. Skin, soft tissue, and wound infections
- General considerations
- Skin and soft tissue infections
- Laboratory diagnostic procedures
- Chapter review
- Bibliography
- 76. Normally sterile body fluids, bone and bone marrow, and solid tissues
- Specimens from sterile body sites
- Laboratory diagnostic procedures
- Chapter review
- Bibliography
- VIII. Clinical Laboratory Management
- 77. Quality in the clinical microbiology laboratory
- Quality program
- Specimen collection and transport
- Standard Operating Procedure Manual
- Personnel
- Reference laboratories
- Patient reports
- Proficiency testing
- Performance checks
- Antimicrobial susceptibility tests
- Maintenance of quality control records
- Maintenance of reference quality control stocks
- Quality assurance program
- Types of quality assurance audits
- Conducting a quality assurance audit
- Continuous daily monitoring
- Chapter review
- Bibliography
- 78. Infection control
- Incidence of health care–associated infections
- Types of health care–associated infections
- Emergence of antibiotic-resistant microorganisms
- Hospital infection control programs
- Role of the microbiology laboratory
- Characterizing strains involved in an outbreak
- Preventing health care–associated infections
- Surveillance methods
- Chapter review
- Bibliography
- 79. Sentinel laboratory response to bioterrorism
- General considerations
- Government laws and regulations
- Laboratory response network
- Chapter review
- Bibliography
- Glossary
- Index
- Edition: 14
- Published: December 28, 2015
- Imprint: Mosby
- Language: English
- eBook ISBN: 9780323428927
PT
Patricia M. Tille
Department Vice Chair, Clinical and Health Information Sciences, Graduate Program Director/Professor, Medical Laboratory Science, University of Cincinnati, Cincinnati, Ohio; Chair, Microbiology Advisory Committee, International Federation of Biomedical Laboratory Science; Editor in Chief, International Journal of Biomedical Laboratory Science, International Federation of Biomedical Laboratory Science; President, American Society of Clinical Laboratory Science.
Affiliations and expertise
Department Vice Chair, Clinical and Health Information Sciences, Graduate Program Director/Professor, Medical Laboratory Science, University of Cincinnati, Cincinnati, Ohio; Chair, Microbiology Advisory Committee, International Federation of Biomedical Laboratory Science; Editor in Chief, International Journal of Biomedical Laboratory Science, International Federation of Biomedical Laboratory Science; President, American Society of Clinical Laboratory Science, USA