Bacterial Laccases

Engineering, Immobilization, Heterologous Production, and Industrial Applications

1st Edition - November 13, 2023
Imprint: Academic Press
Editors: Deepti Yadav, Tukayi Kudanga
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 8 8 9 - 3
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 1 4 5 7 - 4

Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to indust… Read more

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Bacterial Laccases: Engineering, Immobilization, Heterologous Production, and Industrial Applications provides a list of approaches that upgrades bacterial laccases to industrially relevant enzymes. Providing protocols for enzyme production and downstream processing, and including up-to-date information on bacterial laccases, as well as techniques that can be explored to enhance properties and production levels, this book clarifies research gaps and explains how they can be dealt with. Written for research scholars working on enzymes, this is a valuable tool for industries and policymakers.

Cover image
Title page
Table of Contents
Copyright
Dedication
List of contributors
Preface
Chapter 1. Bacterial laccases: a general introduction
Abstract
1.1 Introduction
1.2 Laccase active site and reaction mechanism
1.3 Laccase sources
1.4 Brief review of applications of laccases
1.5 Prospects for bacterial laccases
1.6 Conclusion
References
Chapter 2. Agro-industrial wastes in bacterial laccase production by submerged and solid-state fermentation
Abstract
2.1 Introduction
2.2 Mode of action of laccases
2.3 Industrial importance of bacterial laccases
2.4 Bioprocesses for laccase production using agro-industrial wastes
2.5 Laccase production by solid-state fermentation
2.6 Laccase production by submerged fermentation
2.7 Economics of enzyme production
2.8 Conclusion
References
Chapter 3. Recombinant laccase production: Escherichia coli, Pichia pastoris, and filamentous fungi as microbial factories
Abstract
3.1 Introduction
3.2 Strategies for efficient recombinant protein production
3.3 Different strategies for recombinant laccase production
3.4 Conclusion
References
Chapter 4. Challenges in recovery and purification of laccases
Abstract
4.1 Introduction
4.2 Recovery and purification of extracellular and intracellular laccases
4.3 Challenges in the recovery and purification of laccases
4.4 Preferred solutions to the challenges of laccase recovery and purification
4.5 Conclusion
References
Chapter 5. Laccase engineering: tailoring laccases for effective and efficient catalysis
Abstract
5.1 Introduction
5.2 Mechanism of action
5.3 Structure and catalysis
5.4 Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme
5.5 Approaches to alter laccase properties
5.6 Protein engineering: the search for a robust biocatalyst
5.7 Elucidation of the structure-function relationship of modified enzymes by in-silico tools
5.8 OB-1 Laccases
5.9 Consensus design of OB-1 laccases
5.10 Advantages of engineered laccases over natural laccases
5.11 Improvement in substrate specificity and catalytic activity
5.12 Enhancement of heterologous expression levels
5.13 Enhancement of stability of laccases
5.14 Conclusion
References
Further reading
Chapter 6. Immobilization for enhancement of laccase reusability
Abstract
6.1 Immobilization: trapping enzymes
6.2 Immobilization of bacterial laccases
6.3 Matrices for immobilization
6.4 Arsenal of bacterial laccases: future perspectives and novel applications
6.5 Conclusion
References
Chapter 7. Emerging contaminants and their possible bioremediation through bacterial laccases
Abstract
7.1 Introduction
7.2 Emerging contaminants and their risks
7.3 Treatment methodologies for emerging contaminants
7.4 Enzymes applied in bioremediation of emerging contaminants
7.5 Bacterial laccases applied for the bioremediation of emerging contaminants
7.6 Concluding remarks
Statements and declarations
References
Chapter 8. Bacterial laccases as versatile catalysts in material surface functionalization
Abstract
8.1 Introduction
8.2 Laccase synthesis of surface coatings
8.3 Laccase synthesis of dyes
8.4 Laccase in hair dyeing: synthesis and formulations
8.5 Laccases as bioactive oxygen scavenging coating barriers
8.6 Laccase application in increasing biobased material surface bonding
8.7 Laccases in pulp and paper processing
8.8 Laccase-mediated surface modification of synthetic polymers
8.9 Conclusions and future perspectives
References
Chapter 9. Bacterial laccase-like multicopper oxidases in delignification and detoxification processes
Abstract
9.1 Introduction
9.2 Lignocellulose as a renewable resource
9.3 Lignocellulosic biorefineries
9.4 Pulp and paper industry
9.5 Bacterial multicopper oxidases and their biochemical properties
9.6 The laccase mediator system
9.7 Application of bacterial multicopper oxidases in delignification and detoxification processes
9.8 Taking the next step: finding the “best-fit” bacterial multicopper oxidase for lignocellulosic biorefineries
9.9 Conclusion
Acknowledgements
References
Chapter 10. Laccases in organic synthesis
Abstract
10.1 Introduction
10.2 Laccase historical timeline
10.3 Synthesis of bioactive compounds
10.4 The future of laccase in organic synthesis
10.5 Concluding remarks
References
Chapter 11. Versatility of microbial laccases in industrial applications
Abstract
11.1 Introduction
11.2 Laccase-catalyzed organic synthesis
11.3 Biosensors and medical devices
11.4 Medical applications, healthcare, and cosmetics
11.5 Textiles and leather
11.6 Beverage and food
11.7 Limitations and future perspectives
Acknowledgments
References
Index

Deepti Yadav

Dr. Deepti Yadav grew up in Lucknow, Uttar Pradesh, India. After getting a master’s degree in microbiology from Delhi University, she joined the Durban University of Technology in South Africa to pursue her PhD in biotechnology with Prof. Tukayi Kudanga’s research group within the Department of Biotechnology and Food Science.

Dr. Yadav has worked in a number of research labs, where she gained hands-on experience in techniques such as molecular biology, tissue culture, cloning, protein expression in recombinant hosts and protein purification. She is particularly interested in the fields of enzyme biotechnology, enzyme kinetics, and enzyme applications.

After obtaining her PhD degree, Dr. Yadav was hired as a Research Associate by the School of Energy Materials at Mahatma Gandhi University, Kottayam, where she is working on synthesis of polyhydroxybutyrate from a locally available water hyacinth plant. She has written several scholarly papers and has been invited to speak at conferences. She has also coached and encouraged numerous young students to seek professions in biotechnology.

Affiliations and expertise

Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa

Tukayi Kudanga

Prof. Tukayi Kudanga holds a PhD in biotechnology and is a professor in the Department of Biotechnology and Food Science at Durban University of Technology, South Africa. He is a recognized authority in laccase applications, particularly in biocatalysis and surface functionalization of lignocellulose materials. His areas of expertise include enzyme biotechnology, biocatalysis, and biomaterials. His current research focuses on enzymatic synthesis of bioactive compounds, enzymatic modification of food biopolymers, and development of green technologies for functionalization of lignocellulose materials. He is also interested in the search for novel biocatalysts, developing new applications and modifying enzymes for relevant biotechnological applications. Prof. Kudanga has extensive experience in teaching and research and has published his works widely. He has been invited to be plenary speaker at international conferences and reviews for several scientific journals.

Affiliations and expertise

Professor, Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa

Life Sciences

Physical Sciences & Engineering

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