Bacterial Efflux Systems
- 1st Edition, Volume 722 - November 15, 2025
- Imprint: Academic Press
- Editors: David Christianson, Ben Luisi
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 4 2 9 5 9 - 0
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 4 2 9 6 0 - 6
The volume covers experimental and computational approaches to study the function, structure and mechanism of bacterial efflux pump systems. The methods covered include preparation… Read more
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Request a sales quoteThe volume covers experimental and computational approaches to study the function, structure and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses, and in vivo activity analysis. Essays for evaluating activity are described, including reconstitution of system for quantitative analysis of efflux activity. The topics include sample preparation and analysis for structure determination of pump components and assemblies and with bound ligands and inhibitors, by x-ray crystallography and electron cryomicroscopy. Methods for in situ structural analysis by electron tomography are also described. Topics include partners of the efflux systems and the biogenesis of the nanomachines.
- Interdisciplinary approaches for analysis of structure-mechanism of the complex nanomachines
- Approaches to study function in vivo
- Methods for reconstitution of nanomachines and measure activity
Investigators who are studying bacterial drug resistance
1. Efflux pump functional assays
2. NMR and functional analysis efflux pumps
3. Methods for saturation mutagenesis and functional characterisation
4. Cooperation and functional interplay between pumps
5. Mapping interactions, HDX-MS
Section II: Recombinant Expression and purification
6. Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach
7. Reconstitution of rnd and mfs efflux pumps for structural biology
8. CusCBA system
9. Expression and purification of efflux pumps of Gram positives
10. Expression and purification of efflux pumps of archaea
11. ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc
Section III. Structural and computational studies
12. In situ structural analysis of efflux assemblies by cryoET
13. Molecular dynamics simulations
14. CryoEM analysis of efflux pumps
15. Crystallographic studies of transporters with transport substrates and inhibitors
Section IV. In vivo expression and activity and physiological roles
16. Assembly and biogenesis
17. Heterogeneity of efflux pump expression in populations
2. NMR and functional analysis efflux pumps
3. Methods for saturation mutagenesis and functional characterisation
4. Cooperation and functional interplay between pumps
5. Mapping interactions, HDX-MS
Section II: Recombinant Expression and purification
6. Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach
7. Reconstitution of rnd and mfs efflux pumps for structural biology
8. CusCBA system
9. Expression and purification of efflux pumps of Gram positives
10. Expression and purification of efflux pumps of archaea
11. ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc
Section III. Structural and computational studies
12. In situ structural analysis of efflux assemblies by cryoET
13. Molecular dynamics simulations
14. CryoEM analysis of efflux pumps
15. Crystallographic studies of transporters with transport substrates and inhibitors
Section IV. In vivo expression and activity and physiological roles
16. Assembly and biogenesis
17. Heterogeneity of efflux pump expression in populations
- Edition: 1
- Volume: 722
- Published: November 15, 2025
- Imprint: Academic Press
- No. of pages: 412
- Language: English
- Hardback ISBN: 9780443429590
- eBook ISBN: 9780443429606
DC
David Christianson
After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.
Affiliations and expertise
University of Pennsylvania, USABL
Ben Luisi
Ben Luisi works in the Department of Biochemistry at the University of Cambridge, where he has an appointment as professor of structural biology. He and his colleagues study the structure and mechanism of efflux pumps and the riboregulatory machinery that help to control the expression of the pumps and other complex systems in bacteria.
Affiliations and expertise
Department of Biochemistry, University of Cambridge, U.K.