Bacterial Bioflocculant for Multifunctional Features
- 1st Edition - January 31, 2022
- Imprint: Academic Press
- Authors: S. Sivaramakrishnan, R.T.V. Vimala
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 1 1 3 8 - 2
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 1 3 8 4 - 3
Bacterial Bioflocculant for Multifunctional Features highlights research findings on the production and characterization of self-assembling bioflocculant from bacterial consortiu… Read more
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Bacterial Bioflocculant for Multifunctional Features highlights research findings on the production and characterization of self-assembling bioflocculant from bacterial consortium (encompassing Bacillus subtilis, Enterococcus faecalis and Proteus mirabilis). The book describes the various high-throughput techniques for characterization of wastewater at microbiological and molecular level. Sections cover pharmaceutical compounds, macromolecular compounds and other contaminants, the biotoxicity exhibited cellular and nuclear abnormalities in the zebra fish, and high-throughput techniques used for evaluating the flocculating efficiency of the bacterial bioflocculant to remove the contaminants in different other applications.
Bacterial Bioflocculant for Multifunctional Features will help users undertake further advanced research in bacterial bioflocculant for bioremediation technology and environmental prospectives. In addition, it will also inspire readers to understand bioflocculation and its functions.
- Offers alternative, less expensive biotechnologies where wastewater can be reused
- Focuses on the multipotent bacterial bioflocculant which plays a decisive role in bioremediation
- Discusses techniques for microbially decontaminating polluted wastewater to increase re-usability
Academic faculties, researchers, students and scientists in the field of biotechnology, microbiology, biological science, environmental science, industrial engineering and technology, molecular biology, marine science and technology, and those involved with wastewater management
1: Introduction.
2: Biosynthesis of Bioflocculant from Bacterial Consortium of Municipal Wastewater and its Characterization
2.1. Introduction.
2.2. Isolation of Bioflocculant-producing Bacteria.
2.3. Identification of Effective Bacterial Strains.
2.4. Composition of Bacterial Strains.
2.5. Construction of Bacterial Consortia.
2.6. Composition of Bacterial Consortia.
2.7. Nutritional Growth Medium.
2.8. Optimization of Culture Medium for Bacterial Bioflocculant Production.
2.8.1. Effect of Carbon and Nitrogen Sources.
2.8.2. Effect of Metal Ions.
2.8.3. Effect of Initial pH.
2.8.4. Effect of Time.
2.9. Purification of Bioflocculant.
2.10. Mechanism of Bioflocculant Synthesis.
2.11. Factors Influencing the Flocculating Activity of Bioflocculant.
2.11.1. Dosage.
2.11.2. Metal Ions.
2.11.2. Temperature.
2.11.3. pH.
2.12. Determination of Flocculating Activity.
2.13. Functional Characterization of Bioflocculant.
2.13.1. Compositional Characterization.
2.13.2. Chemical Characterization.
2.13.3. Molecular Characterization.
2.13.4. Biochemical Characterization.
References.
3: Characterization of Complex Wastewater Using High-Throughput Techniques
3.1. Introduction.
3.2. Physicochemical Characteristics.
3.3. Molecular Characterization.
3.4. Analysis of Organic Compounds.
3.4.1. High-performance Gas Chromatography-Mass Spectrometry (GC-MS) Analysis.
3.5. Analysis of Pharmaceutical Compounds.
3.5.1. High-resolution Liquid Chromatography- Mass Spectrometry (HR-LCMS) Analysis.
3.6. Analysis of Functional Groups of Organic Compounds.
3.6.1. X-ray Photoelectron Spectroscopy (XPS) Analysis.
3.6.2. Fourier Transform Infrared Spectroscopy (FTIR) Analysis.
3.7. Analysis of Particle Size Distribution.
3.7.1. Dynamic Light Scattering (DLS) Analysis.
3.7.2. Particle Size Distribution (PSD) Analysis.
3.8. Analysis of Elements.
3.8.1. Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray
Spectroscopy (FE-SEM-EDAX) Analysis.
3.8.2. X-ray Diffraction (XRD) Analysis.
3.8.3. Three-Dimensional Fluorescence Excitation-Emission Matrix (3D-EEM) Analysis.
3.9. Toxicity Studies using Zebra Fish.
3.9.1. Comet (CM) Assay.
3.9.2. Micronucleus (MN) Assay.
3.10. Conclusion
References.
4: Bacterial Bioflocculant as Bioengineers in Wastewater Treatment
4.1. Introduction.
4.2. Removal of Organic Compounds.
4.2.1. GC-MS Analysis.
4.2.2. HR-LCMS Analysis.
4.2.3. FTIR Analysis.
4.3. Removal of Fluorescent Compounds.
4.3.1. 3D-EEM Analysis.
4.4. Removal of Elements.
4.4.1. XPS Analysis.
4.4.2. XRD Analysis.
4.4.3. SEM Analysis.
4.5. Removal of Particles.
4.5.1. PSD with Zeta Potential Analysis.
4.6. Detoxicity Studies in Zebra Fish.
4.6.1. Histopathology.
4.6.2. Genotoxicity Assay.
4.6.2.1. CM Assay.
4.6.2.2. MN Assay.
4.7. Antimicrobial Efficiency.
4.8. Summary.
References.
5: Application of Bacterial Bioflocculant for the Synthesis of Biocapped Metal Nanoparticles and their Multifunctional Features
5.1. Bioflocculant and Bio@MNPs Interaction.
5.2. Characterization of Bioflocculant and Bio@MNPs.
5.3. Multifunctional Features of Bacterial Bioflocculant and Bio@MNPs.
5.3.1. Antitumor Activity.
5.3.2. Biosorption for Heavy Metal Removal.
5.3.3. Mosquitocidal Activity.
5.3.4. Biofilm Inhibition Activity.
References.
6: Implications of Bacterial Bioflocculant for Public Health Demands
6.1. Elimination of Microbial Pathogens in Contaminated Water.
6.2. Eradication of Mosquito Breeding Sites in Wastewater.
6.3. Management of Industrial Effluents.
6.4. Recycling of Agricultural Wastes.
6.5. Recent Advancements in Miscellaneous Applications.
References.
7: Summary
- Edition: 1
- Published: January 31, 2022
- Imprint: Academic Press
- Language: English
SS
S. Sivaramakrishnan
Dr. S. Sivaramakrishnan is engaged in research (more than 20 years) and teaching. His specialization is in Agricultural Biotechnology in relation to biopesticides and Nanobiotechnology towards biomedical applications. He has produced 16 Ph.D.s and guiding M.Sc., M.Phil. and Ph. D. and M.Tech. students for their research work. He has won young scientists award four times. He is a member of the editorial board of European Journal of Nematology and is a reviewer for nearly ten journals. He has scientific publications in national and international reputed journals. He has been the principal investigator of major DBT, DST and UGC projects. He has international collaborations, signed MoU between USA, Korea, Iran and Turkey. He has organized international conferences and national awareness programme
Affiliations and expertise
Associate Professor and Head, Department of Biotechnology Bharathidasan University Tiruchirappalli, Tamil Nadu, IndiaRV
R.T.V. Vimala
Dr. R.T.V. Vimala is specialized in Biotechnology. She is a young energetic emerging scientist in the field, engaged in teaching and research activities. She has scientific papers published in reputed journals. She has submitted ten bacterial sequences in NCBI.
Affiliations and expertise
Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, IndiaRead Bacterial Bioflocculant for Multifunctional Features on ScienceDirect