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Autoradiography at the Cellular Level
Physical Techniques in Biological Research
- 2nd Edition - October 22, 2013
- Author: Brigitte Schultze
- Editor: Arthur W. Pollister
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 1 - 7 9 0 6 - 3
- eBook ISBN:9 7 8 - 1 - 4 8 3 1 - 9 4 8 7 - 5
Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This… Read more
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Request a sales quotePhysical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium. This book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms. The topics include the characteristics of the different types of radiation; autoradiographic resolution; techniques for applying photographic emulsions; and autoradiography of water-soluble substances. The application of different isotopes; general aspects of the use of thymidine; physiological RNA synthesis; and RNA synthesis under pathological conditions are also elaborated. This text likewise covers the protein synthesis under normal conditions; electron microscopic autoradiography; application of electron microscopic autoradiography; and autoradiographic methods in cytochemistry. This publication is a good reference for students, researchers, and clinicians conducting work on the use of tritiated compounds in high resolution autoradiography.
PrefacePreface to Second Edition of Volume III1. General and Theoretical Aspects of Autoradiography I. Characteristics of the Different Types of Radiation II. Autoradiographic Emulsions III. Photographic Process IV. Autoradiographic Resolution V. Dosage of Isotopes VI. Background and Artifacts VII. Quantitative Autoradiography2. Autoradiographic Techniques I. Preparation of Tissue II. Techniques for Applying Photographic Emulsions III. Exposure IV. Development and Fixation V. Staining VI. Autoradiography of Water-Soluble Substances3. Application of Different Isotopes I. Iodine-131 and Iodine-125 II. Sulfur-35 III. Phosphorus-32 IV. Carbon-14 V. Iron-59 VI. Calcium-45 VII. Strontium-90, Strontium-89, and Strontium-85 VIII. Other Isotopes IX. Water-Soluble Cations and Anions4. DNA Synthesis I. General Aspects of the Use of Thymidine II. Autoradiographic Studies of the Cellular DNA Synthesis with Labeled Thymidine5. RNA Synthesis I. General Aspects of Autoradiographic Studies on RNA Synthesis II. Physiological RNA Synthesis III. RNA Synthesis Under Pathological Conditions IV. RNA Synthesis After X-Ray Irradiation6. Protein Synthesis I. Correlation Between Amino Acid Incorporation Rate and the Specific Activity of the Free Amino Acid II. Protein Synthesis Under Normal Conditions III. Protein Synthesis Under Special Conditions7 . Lipid Synthesis Text8. Electron Microscopic Autoradiography I. General Considerations of the Method II. Technique III. Application of Electron Microscopic Autoradiography9. Autoradiographic Methods in CytochemistryBibliographyAdditional References Author IndexSubject Index
- No. of pages: 212
- Language: English
- Edition: 2
- Published: October 22, 2013
- Imprint: Academic Press
- Paperback ISBN: 9781483179063
- eBook ISBN: 9781483194875
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