Atomic Force Microscopy for Nanoscale Biophysics
From Single Molecules to Living Cells
- 1st Edition - February 15, 2023
- Imprint: Academic Press
- Author: Mi Li
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 5 3 6 0 - 3
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 5 8 3 3 - 2
Atomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomol… Read more
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Request a sales quoteAtomic Force Microscopy for Nanoscale Biophysics: From Single Molecules to Living Cells summarizes the applications of atomic force microscopy for the investigation of biomolecules and cells. The book discusses the methodology of AFM-based biomedical detection, diverse biological systems, and the combination of AFM with other complementary techniques. These state-of-the-art chapters empower researchers to address biological issues through the application of atomic force microscopy. Atomic force microscopy (AFM) is a unique, multifunctional tool for investigating the structures and properties of living biological systems under aqueous conditions with unprecedented spatiotemporal resolution.
- Summarizes the recent progress of atomic force microscopy in biomedical applications
- Presents the methods and skills of applying atomic force microscopy
- Aids researchers in investigating the nanoscale biophysics of diverse biological systems
Researchers in Biophysics, Cell Biology, Molecular Biology, Biochemistry, Material Science and related Biomedical fields. Graduate Students
- Cover image
- Title page
- Table of Contents
- Copyright
- About the author
- Preface
- Chapter 1. Fundamentals and methods of atomic force microscopy for biophysics
- Abstract
- 1.1 Background of atomic force microscopy for biophysics
- 1.2 Atomic force microscopy topographical imaging modes
- 1.3 Atomic force microscopy force spectroscopy techniques
- 1.4 High-speed atomic force microscopy
- 1.5 Topography and recognition imaging mode atomic force microcopy
- References
- Chapter 2. Imaging and force detection of single deoxyribonucleic acid molecules by atomic force microscopy
- Abstract
- 2.1 Background
- 2.2 Sample preparation methods
- 2.3 Topographical imaging of single DNA molecules and events
- 2.4 Time-lapse imaging of individual DNA molecular dynamics
- 2.5 Extracting the persistence length of DNA molecules from atomic force microscopy images
- 2.6 Mechanically unzipping single DNA molecules by atomic force microscopy force spectroscopy
- 2.7 Probing individual DNA behaviors on DNA origami nanostructures
- 2.8 Summary
- References
- Chapter 3. High-resolution imaging and force spectroscopy of single membrane proteins by atomic force microscopy
- Abstract
- 3.1 Background
- 3.2 Topographical imaging of single native membrane proteins
- 3.3 Unfolding mechanics of individual native membrane proteins
- 3.4 Observing the dynamics of single membrane proteins by high-speed atomic force microscopy
- 3.5 Multiparametric atomic force microscopy imaging of single membrane proteins
- 3.6 Topography and recognition imaging of single membrane proteins
- 3.7 Summary
- References
- Chapter 4. Characterizing the nanostructures and mechanical properties of hydrogels by atomic force microscopy
- Abstract
- 4.1 Background
- 4.2 Nanostructures and nanomechanics of natural plant hydrogels
- 4.3 Characterizations of biopolymeric hydrogels inspired by carnivorous plant mucilage
- 4.4 Imaging and mechanical analysis of peptide-assembled nanofibrillar hydrogel
- 4.5 Probing the mechanical cues in cell–hydrogel interactions
- 4.6 Summary
- References
- Chapter 5. Detecting the behaviors of single viruses by atomic force microscopy
- Abstract
- 5.1 Background
- 5.2 Imaging the fine structures of single viruses
- 5.3 Nanoindentation for mechanical measurements and manipulations of single viruses
- 5.4 Single-virus force spectroscopy for probing viral binding affinity
- 5.5 Multiparametric atomic force microscopy imaging of virus–cell interactions
- 5.6 Visualizing individual viral dynamics by high-speed atomic force microscopy
- 5.7 Summary
- References
- Chapter 6. Imaging and mechanical analysis of single native exosomes by atomic force microscopy
- Abstract
- 6.1 Background
- 6.2 Exosome isolation and immobilization
- 6.3 Imaging single native exosomes in liquid
- 6.4 Measuring the mechanics of single native exosomes
- 6.5 Multiparametric imaging of single native exosomes
- 6.6 Single-molecule force spectroscopy on single exosomes
- 6.7 Summary
- References
- Chapter 7. Nanoscale imaging and force probing of single microbial cells by atomic force microscopy
- Abstract
- 7.1 Background
- 7.2 Immobilization methods of living microbial cells for atomic force microscopy imaging
- 7.3 Visualizing the nanostructures and their dynamics of living microbial cells by atomic force microscopy
- 7.4 Measuring the mechanical properties of single living microbial cells by atomic force microscopy
- 7.5 Single-molecule force spectroscopy and single-cell force spectroscopy of microbial adhesion
- 7.6 Multiparametric atomic force microscopy imaging of single living microbial cells
- 7.7 Atomic force microscopy cantilever as a nanomechanical sensor for monitoring microbial activities
- 7.8 Summary
- References
- Chapter 8. Investigating the structures and mechanics of single animal cells by atomic force microscopy
- Abstract
- 8.1 Background
- 8.2 Imaging the surface structures and their dynamics of single living adherent animal cells
- 8.3 Measuring the mechanical properties of single living adherent animal cells
- 8.4 Probing the molecular activities on the surface of single adherent cells
- 8.5 Visualizing the surface structures and their dynamics of single living suspended animal cells
- 8.6 Detecting the mechanical cues involved in the activities of lymphoma cells
- 8.7 Probing the molecular activities on the surface of primary lymphoma cells
- 8.8 Summary
- References
- Chapter 9. Characterizing the extracellular matrix for regulating cell behaviors by atomic force microscopy
- Abstract
- 9.1 Background
- 9.2 Detecting the mechanical properties of decellularized extracellular matrix
- 9.3 Investigating the structures and mechanics of basement membranes
- 9.4 In situ imaging of cell culture medium-forming nanogranular surface for cell growth
- 9.5 Hierarchical micro-/nanotopography of extracellular matrix for tuning cellular structures and mechanics
- 9.6 Summary
- References
- Chapter 10. Combining atomic force microscopy with complementary techniques for biophysics
- Abstract
- 10.1 Background
- 10.2 Scanning near-field ultrasound holography
- 10.3 Fluidic force microscopy
- 10.4 Combining atomic force microscopy with micropipette
- 10.5 Combining atomic force microscopy with fluidic environment
- 10.6 Summary
- References
- Chapter 11. Future perspectives of atomic force microscopy for biophysics
- Abstract
- References
- Index
- Edition: 1
- Published: February 15, 2023
- Imprint: Academic Press
- No. of pages: 336
- Language: English
- Paperback ISBN: 9780323953603
- eBook ISBN: 9780323958332
ML
Mi Li
Mi Li PhD is a Full Professor at Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, China. Mi Li received the Ph.D. degree in 2015 from Shenyang Institute of Automation (SIA) Chinese Academy of Sciences, Shenyang, China, after which he continues academic career at SIA until now. Since 2009, Mi Li has been engaged in the studies of atomic force microscopy (AFM) and its biomedical applications ranging from molecules and organelles to cells as well as hydrogels, to reveal the role of micro/nanoscale biophysical cues in regulating life activities. Mi Li has conducted long-term visitor studies at The Ohio State University, Columbus, USA and ETH Zurich, Switzerland, respectively for academic research regarding AFM. Mi Li has won the National Natural Science Foundation of China for Excellent Young Scholars (2019), the IEEE Senior Member (2020), the Outstanding Doctoral Dissertation Award of Chinese Academy of Sciences (2016) and the Springer Theses (2018).
Affiliations and expertise
Professor, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, Liaoning province, ChinaRead Atomic Force Microscopy for Nanoscale Biophysics on ScienceDirect