Assessment of Immune status by the Leukocyte Adherence Inhibition Test

1st Edition - January 1, 1982
Imprint: Academic Press
Editor: D Thomson
Language: English
Hardback ISBN:
9 7 8 - 0 - 1 2 - 6 8 9 7 5 0 - 0
Paperback ISBN:
9 7 8 - 0 - 1 2 - 4 3 1 4 2 8 - 3
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 5 2 2 9 - 7

Assessment of Immune Status by the Leukocyte Adherence Inhibition Test reviews many aspects of the immune response and cellular requirements in leukocyte adherence inhibition… Read more

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Assessment of Immune Status by the Leukocyte Adherence Inhibition Test reviews many aspects of the immune response and cellular requirements in leukocyte adherence inhibition (LAI). It compares the LAI methodology with alternative approaches, discusses the method that has been adopted to determine whether the LAI phenomenon measures a specific immune response, and explores how these phenomena fit in with and relate to the traditional immune response. Organized into three parts encompassing 15 chapters, this volume begins with an overview of the hemocytometer assay, the immunological basis and applications of hemocytometer LAI, the immune response as assayed by the test tube method, and the microplate assay. It then discusses cellular populations in LAI; measurements of humoral antitumor immunity; clinical application of LAI in breast cancer, prostate cancer, and rheumatic diseases; the biology of LAI and its response to human cancer; and cellular and pharmacologic aspects of LAI. It also compares microplate LAI with other in vitro assays of cell-mediated immunity, as well as tumor immunity in animal models and human cancer. Both beginning and experienced investigators involved in all aspects of immunologic research will find this book extremely useful.

List of Contributors

Preface

I Leukocyte Adherence Inhibition on Hemocytometers

1 Hemocytometer LAI: Immunological Basis and Applications

I. Introduction

II. Historical Survey

III. Technical Summary

IV. Reaction Mechanism

V. Immunodiagnosis of Human Cancer

VI. Some Comparisons and Relationships

VII. Future Prospects

References

2 Cellular Populations in Leukocyte Adherence Inhibition

I. Introduction

II. LAI Methods

III. Cellular Interactions

IV. Quantitative Aspects

V. Conclusions

References

3 Measurements of Humoral Antitumor Immunity

I. Introduction

II. H-LAI Measurements

III. Nature of Humoral Factor

IV. Mechanisms of the H-LAI Response

V. H-LAI Measurements in Cancer Detection

VI. Conclusions

References

4 Clinical Application in Breast Cancer

I. Introduction

II. Antigen Preparation

III. Specificity of LAI Assay

IV. Mechanism of LAI

V. LAI Assay among Family Members of Breast Cancer Patients

VI. LAI and Serum Blocking Factors

VII. Arming Phenomenon with Breast Cancer Patients' Sera

VIII. Evolution of LAI at the City of Hope

IX. Conclusion

References

II Leukocyte Adherence Inhibition in Test Tubes

5 The Use of the Assay to Measure Immunity in Animal Models

I. Introduction

II. Methodological Aspects

III. Specificity, Mechanism, and Biological Significance

IV. Discussion

References

6 The LAI Response to Human Cancer: The Biology of the LAI Response and Features of the Antigens

I. Introduction

II. Computerized Tube LAI Assay

III. Oncofetal Organ-Specific Neoantigens of Human Cancer

IV. Biology of the LAI Response

V. Modulation of the Expression of Human Antitumor Immunity

VI. Diagnostic Value

VII. Summary

References

7 Leukocyte Adherence Inhibition, Carcinoembryonic Antigen, and Tumor Size in Patients with Colorectal Carcinoma

I. Introduction

II. Methods and Technical Problems

III. Results

References

8 Evaluation of Leukocyte Adherence Inhibition in Prostate Cancer

I. Introduction

II. Hemocytometer LAI

III. Tube LAI

IV. Disease-Specific Reactivity

V. Tumor-Specific Reactivity

VI. Effect of Therapy

VII. Evaluation of LAI Data

VIII. Conclusion

References

9 The Leukocyte Adherence Inhibition Assay in Human and Experimental Rheumatic Diseases

I. Arthritic Disorders Evaluated Utilizing LAI Assay

II. Standardization of the Tube LAI Assay

III. Results of LAI Assay in Rheumatoid Arthritis

IV. Results of LAI Assay in Experimental Arthritis

V. Significance of LAI Studies in Experimental Arthritis

VI. Significance of LAI Studies in Rheumatoid Arthritis

VII. Conclusions

References

10 Cellular and Pharmacologic Aspects

I. Introduction

II. The Test in Arthritis

III. Significance of the Nonadherence in the LAI

IV. Cellular Aspects

V. Sensitization of IgG Rheumatoid Factor

VI. LAI Test and Pregnancy Serum

VII. Characterization of Synovial Extract

VIII. Activation of Polymorphonuclear Leukocytes

IX. Leukocyte Adherence and Prostaglandins

X. Leukocyte Adherence and ß Receptors

XI. LAI Test with Synovial Fluid Leukocytes

XII. Adherence and Immunomodulation

XIII. Discussion

References

III Leukocyte Adherence Inhibition in the Microplate

11 Comparison of Microplate LAI with Other in Vitro Assays of Cell-Mediated Immunity: Assessment of Responses to Bacterial, Viral, and Tumor Antigens in Man and Experimental Animals

I. Microplate LAI

II. Microplate LAI Reactivity in Murine Tumor Systems

III. Microplate LAI in the Assessment of Host Responses to Viral and Bacterial Antigens

IV. Conclusions

References

12 A Comparison of Tumor Immunity in Animal Models and Human Cancer

I. Introduction

II. Method

III. Characterization of Antigen(s) Detected in the Microplate LAI Assay

IV. Patterns of Sensitization Detected by the Microplate LAI Assay

V. Serum Studies

VI. Mechanism of Reactivity

VII. Summary

References

13 Cellular Requirements for the Leukocyte Adherence Inhibition Response to an Immunogenic Viral Induced Murine Tumor

I. Introduction

II. Methodology

III. LAI Response of Mice to Regressing MSV-lnduced Tumors

IV. LAI Response to Progressively Growing Tumors

V. Mechanisms of the LAI Response to MSV-lnduced Tumors

VI. Comparison with Mouse MIF Formation

VII. Conclusions

References

14 Leukocyte Adherence Inhibition Testing in Domestic Animals

I. Introduction

II. Methods Available for the Evaluation of Cellular Immunity in Domestic Animals

III. LAI Testing

IV. Experimental Use of the LAI Microassay

V. Conclusions

References

15 Leukocyte Adherence Inhibition: Measurement by Radioisotopic Labeled Cells

I. Introduction

II. Assay Using 111Indium-Labeled Cells

References

Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Assessment of Immune status by the Leukocyte Adherence Inhibition Test

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