Artificial Metalloproteins
- 1st Edition, Volume 724 - December 1, 2025
- Imprint: Academic Press
- Editor: Lisa Olshansky
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 3 - 4 1 5 7 3 - 9
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 4 1 5 7 4 - 6
In recent decades, artificial metalloproteins (ArMs) have emerged as powerful vehicles with which to control, implement, and leverage the unique reactivity and spectroscopic… Read more
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In recent decades, artificial metalloproteins (ArMs) have emerged as powerful vehicles with which to control, implement, and leverage the unique reactivity and spectroscopic properties of their bound metal ions. This volume provides a practical guide to the latest ArM research, and is organized in a hierarchical fashion reflecting the primary, secondary, tertiary, and quaternary elements of protein structure. The first two sections provide a wide breadth of strategies for incorporating the primary coordination elements required for metal binding, which are further divided into the installation of heme and non-heme metallocofactor sites. The third section relates to the installation and tuning of secondary coordination sphere effects around the metal center to modulation of properties therein. Finally, the fourth section relates tertiary and quaternary protein design elements to resultant changes in properties of the bound metallocofactors. Ultimately the design, preparation, and characterization of ArMs at all of these hierarchical levels are needed to recapitulate the structures and functions of natural metalloproteins. By providing a detailed roadmap for achieving each of these elements, this volume aims to disseminate the cumulative knowledge developed by researchers around the world such that the next generation of ArM researchers can build from it and ultimately, beyond.
- A wide variety of strategies for the installation of nonnative metal binding sites are described
- Protein engineering approaches range from modulating secondary coordination sphere effects around the metal ion, to implementation of peptide dynamical motions for the regulation of these effects
- Provides a ‘how-to’ guide for the interdisciplinary techniques needed in the construction and characterization of diverse ArM systems
Chemistry, Biology, Biophysics, and Bioengineering students, educators, and researchers who are interested in understanding and applying metalloenzyme and metalloprotein reactivity, structure, and function
1. Incorporating Non-Native Cofactors into Heme Proteins
Christopher Lemon
2. Designing 4-Helix Bundles to Bind Porphyrin/Porphyrin-like Cofactors
Samuel Mann
3. Cobalt-Substituted Hemoprotein Expression
Andrew Buller
4. Rationally designed directed evolution of cytochrome P450 enzymes
Shyamalava Mazumdar
5. De novo design of a switchable 𝛼-helical hemoprotein
Vincent Louis Pecoraro
Primary Coordination Sphere Part B: Unnatural Metal-Binding Sites Beyond the Heme
6. Unnatural Amino Acid Incorporation for Creating Artificial Metalloenzymes
Woon Ju Song
7. Development of a PQQ-Dependent Artificial Metalloprotein
Lisa Olshansky
8. Evolutionary Engineering of a Rhodium Complex-Linked Artificial Metalloenzyme
Akira Onoda
9. Design and Application of Nonheme Iron Enzymes
Xiongyi Huang
Part C: Secondary Sphere Modulation for Metallocofactor Activation
10. Designing Artificial Metalloenzymes by Engineering the Primary and Secondary Coordination Sphere in Native Protein Scaffolds
Yi Lu
11. De Novo-Designed Artificial Cu Proteins and Electron Transfer
Saumen Chakraborty
12. Design and Evaluation of Biocatalysts for Energy-Related Reactions
Kara Bren
Part D. Superstructural Control: Tertiary and Quaternary Effects in Metalloprotein Design
13. Breaking Symmetry: Metal-Guided Assembly of Heterotrimeric Coiled Coils
Vincent Louis Pecoraro and Salvatore La Gatta
14. Engineering Conformationally Switchable Artificial Metalloproteins
Lisa Olshansky
15. Chemical Methods to Stabilize Segments of Metalloproteins in the Form of Peptides
Andy Nguyen
16. Design and Production of De Novo Protein Nanowires
Ross Anderson
Christopher Lemon
2. Designing 4-Helix Bundles to Bind Porphyrin/Porphyrin-like Cofactors
Samuel Mann
3. Cobalt-Substituted Hemoprotein Expression
Andrew Buller
4. Rationally designed directed evolution of cytochrome P450 enzymes
Shyamalava Mazumdar
5. De novo design of a switchable 𝛼-helical hemoprotein
Vincent Louis Pecoraro
Primary Coordination Sphere Part B: Unnatural Metal-Binding Sites Beyond the Heme
6. Unnatural Amino Acid Incorporation for Creating Artificial Metalloenzymes
Woon Ju Song
7. Development of a PQQ-Dependent Artificial Metalloprotein
Lisa Olshansky
8. Evolutionary Engineering of a Rhodium Complex-Linked Artificial Metalloenzyme
Akira Onoda
9. Design and Application of Nonheme Iron Enzymes
Xiongyi Huang
Part C: Secondary Sphere Modulation for Metallocofactor Activation
10. Designing Artificial Metalloenzymes by Engineering the Primary and Secondary Coordination Sphere in Native Protein Scaffolds
Yi Lu
11. De Novo-Designed Artificial Cu Proteins and Electron Transfer
Saumen Chakraborty
12. Design and Evaluation of Biocatalysts for Energy-Related Reactions
Kara Bren
Part D. Superstructural Control: Tertiary and Quaternary Effects in Metalloprotein Design
13. Breaking Symmetry: Metal-Guided Assembly of Heterotrimeric Coiled Coils
Vincent Louis Pecoraro and Salvatore La Gatta
14. Engineering Conformationally Switchable Artificial Metalloproteins
Lisa Olshansky
15. Chemical Methods to Stabilize Segments of Metalloproteins in the Form of Peptides
Andy Nguyen
16. Design and Production of De Novo Protein Nanowires
Ross Anderson
- Edition: 1
- Volume: 724
- Published: December 1, 2025
- Imprint: Academic Press
- Language: English
LO
Lisa Olshansky
After earning her B.S. in Chemistry from UC San Diego in 2009, Dr. Lisa Olshansky completed her Ph.D. in Chemistry at MIT in 2015 as an NSF Graduate Research and Presidential fellow under the mentorship of Profs. Daniel Nocera and JoAnne Stubbe. She then went on to work with Prof. Andy Borovik at UC Irvine as an ACS Irving S. Sigal Postdoctoral fellow. In 2018, Lisa began her independent career as an Assistant Professor of Chemistry at the University of Illinois, Urbana-Champaign where her team is working to mimic and exploit mechanisms by which macroscopic molecular structural changes dictate metal ion electronic structural properties. During her early career, Olshansky has been recognized with Searle, Cottrell, and Vallee Scholars awards, Carver Trust and DOE Young Investigator awards, and an NIH Early Stage Investigator award. Olshansky was recently named an NAS Kavli Fellow, received the Paul Saltman Young Investigator Award for her research on Metals in Biology, and was recently named a Camille Dreyfus Teacher-Scholar and a Sloan Research Fellow. Finally, since joining the faculty at Illinois, Olshansky has spearheaded an initiative called C2 that aims to foster inclusivity and diversity in the School of Chemical Sciences at Illinois.
Affiliations and expertise
Assistant Professor of Chemistry, Department of Chemistry, University of Illinois, USA