Approaches to Research on the Systematics of Fish-Borne Trematodes

Preface

Chapter 1. Medically Important Fish-Borne Zoonotic Trematodes

1.1 Major Fish-Borne Trematodes

1.2 Other Fish-Borne Trematodes

References

Chapter 2. Collection of Fish-Borne Trematodes from the Final Host

2.1 Diagnostic Methods

2.2 Treatment and Purgation

2.3 Collection of Adult FBT from Animals

2.4 Worm Preparation for Morphological Study

2.5 Diagnostic Characteristics of FBT

References

Chapter 3. Collection of Fish-Borne Trematode Cercaria

3.1 Snail Host of Fish-Borne Trematodes

3.2 Cercaria Morphological Study

3.3 Morphology of Fresh Cercaria

3.4 Vital Stain

3.5 Chaetotaxy

3.6 Polymerase Chain Reaction

References

Chapter 4. Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host

4.1 Development of Metacercariae

4.2 Methods of Fish Examination for Metacercariae

4.3 Mobile Laboratory for Fish Examination

4.4 Method of Excysting Metacercariae

4.5 Morphology of Metacercariae

References

Chapter 5. Molecular Systematics of Fish-Borne Trematodes

5.1 What is Molecular Systematics?

5.2 Species Identification: Morphological and Molecular Approaches

5.3 Advantages and Disadvantages of using DNA Sequences for Systematics

5.4 Molecular Markers

5.5 The Regions of DNA Used for Molecular Systematics

5.6 Molecular Systematics of Fish-Borne Trematodes

5.7 Summary and Future Direction

References

Chapter 6. Methods of Molecular Study: DNA Sequence and Phylogenetic Analyses

6.1 Criterion for Selecting Appropriate Genetic Markers

6.2 Phylogenetic Analysis

6.3 How to Construct Phylogenetic Trees

6.4 Further Perspectives

References

Appendix A

References

Appendix B

1 Genomic DNA Preparation

2 Standard Polymerase Chain Reaction (PCR) Protocol

References

Glossary