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1st Edition - February 17, 2014
Authors: Jitra Waikagul, Urusa Thaekham
Approaches to Research on the Systematics of Fish-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish-borne trematodes both in the endemic areas… Read more
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Immediately download your ebook while waiting for your print delivery. No promo code is needed.
Approaches to Research on the Systematics of Fish-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish-borne trematodes both in the endemic areas and experimental laboratories. It includes methods to identify species of fish-borne trematodes to enhance the precision of research studies based on the metacercarial stage.
Misidentification of trematode species is a common occurrence when researchers are new to the field and have no guidance. Consequentially, sometimes publications report inaccurate prevalence rates of these parasites. This compact guide gives clear direction on:
Collection of parasites in the final hosts
Collection of cercaria from snail first intermediate hosts
Collection of metacercaria from fish hosts
Molecular identification of parasites
Systematics of fish-borne trematodes
parasitologists, helminthologists, biologists, ecologists and public health specialists.
Preface
Chapter 1. Medically Important Fish-Borne Zoonotic Trematodes
1.1 Major Fish-Borne Trematodes
1.2 Other Fish-Borne Trematodes
References
Chapter 2. Collection of Fish-Borne Trematodes from the Final Host
2.1 Diagnostic Methods
2.2 Treatment and Purgation
2.3 Collection of Adult FBT from Animals
2.4 Worm Preparation for Morphological Study
2.5 Diagnostic Characteristics of FBT
References
Chapter 3. Collection of Fish-Borne Trematode Cercaria
3.1 Snail Host of Fish-Borne Trematodes
3.2 Cercaria Morphological Study
3.3 Morphology of Fresh Cercaria
3.4 Vital Stain
3.5 Chaetotaxy
3.6 Polymerase Chain Reaction
References
Chapter 4. Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host
4.1 Development of Metacercariae
4.2 Methods of Fish Examination for Metacercariae
4.3 Mobile Laboratory for Fish Examination
4.4 Method of Excysting Metacercariae
4.5 Morphology of Metacercariae
References
Chapter 5. Molecular Systematics of Fish-Borne Trematodes
5.1 What is Molecular Systematics?
5.2 Species Identification: Morphological and Molecular Approaches
5.3 Advantages and Disadvantages of using DNA Sequences for Systematics
5.4 Molecular Markers
5.5 The Regions of DNA Used for Molecular Systematics
5.6 Molecular Systematics of Fish-Borne Trematodes
5.7 Summary and Future Direction
References
Chapter 6. Methods of Molecular Study: DNA Sequence and Phylogenetic Analyses
6.1 Criterion for Selecting Appropriate Genetic Markers
6.2 Phylogenetic Analysis
6.3 How to Construct Phylogenetic Trees
6.4 Further Perspectives
References
Appendix A
References
Appendix B
1 Genomic DNA Preparation
2 Standard Polymerase Chain Reaction (PCR) Protocol
References
Glossary
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