Applied Raman Spectroscopy
Concepts, Instrumentation, Chemometrics, and Life Science Applications
- 1st Edition - March 17, 2025
- Imprint: Elsevier Science
- Editor: Vivek Kumar Singh
- Language: English
- Paperback ISBN:9 7 8 - 0 - 4 4 3 - 2 1 8 3 4 - 7
- eBook ISBN:9 7 8 - 0 - 4 4 3 - 2 1 8 3 5 - 4
Applied Raman Spectroscopy: Concepts, Instrumentation, Chemometrics, and Life Science Applications synthesizes recent developments in the field, providing an updated overview.… Read more
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Request a sales quoteApplied Raman Spectroscopy: Concepts, Instrumentation, Chemometrics, and Life Science Applications synthesizes recent developments in the field, providing an updated overview. The book focuses on the modern concepts of Raman spectroscopy techniques, recent technological innovations, data analysis using chemometric methods, along with the latest examples of life science applications relevant in academia and industries. It will be beneficial to researchers from various branches of science and technology, and it will point them to modern techniques coupled with data analysis methods. In addition, it will help instruct new readers on Raman spectroscopy and hyphenated Raman spectroscopic techniques.
The book is primarily written for analytical and physical chemistry students and researchers at a more advanced level who require a broad introductory overview of the applications of Raman spectroscopy, as well as those working in applied industry and clinical laboratories. Students, researchers, and industry workers in related fields, including X-ray and materials science, agriculture, botany, molecular biology and biotechnology, mineralogy, and environmental science will also find it very useful.
- Provides a thorough discussion of the modern concepts and recent instrumental developments of Raman Spectroscopy in one resource
- Presents comprehensive discussions on laser spectrometers, Raman Spectrometers, and detectors that can be used for apparatuses
- Furnishes the latest updates on remote Raman spectroscopy in nanoscale optics, stimulated Raman microscopy and clinical as well as biomedical applications of surface-enhanced and tip-enhanced Raman spectroscopy
- Covers the newest advances and capabilities of Raman-LIBS instruments, ranging from basic set-ups to more advanced configurations
- Demonstrates updated chemometrics and numerical methods and shows the analytical capabilities of methods in terms of detection limits, accuracy, and precision of measurements for biological and environmental samples
Analytical and physical chemistry students and researchers at a more advanced level who require a broad introductory overview of the applications of Raman Spectroscopy, as well as those working in applied industry and clinical laboratories, Students, researchers, and industry workers in related fields including X-ray and Materials science, agriculture, botany, molecular biology and biotechnology, mineralogy, energy, and environmental science
- Title of Book
- Cover image
- Title page
- Table of Contents
- Copyright
- Dedication
- List of contributors
- Chapter 1. Introduction of basic theory and principle of Raman scattering and spectroscopy
- Abstract
- 1.1 Background
- 1.2 Raman spectroscopy
- 1.3 Conclusion
- Conflicts of interests
- References
- Chapter 2. Overview of the basics of resonance Raman spectroscopy, applications, and recent advances
- Abstract
- 2.1 Introduction
- 2.2 Applications and importance in interdisciplinary research
- 2.3 Theory of resonance Raman spectroscopy
- 2.4 Instrumentation for resonance Raman spectroscopy
- 2.5 Experimental techniques and data analysis
- 2.6 Applications of resonance Raman spectroscopy
- 2.7 Recent advances in resonance Raman spectroscopy
- 2.8 Future perspectives and challenges
- References
- Chapter 3. Remote Raman spectroscopy in nanoscale optics
- Abstract
- 3.1 Introduction
- 3.2 Nanoscale optics and plasmonics
- 3.3 Quantum emitters
- 3.4 Fabrication of plasmonic structures
- 3.5 Plasmon influenced Raman spectroscopy
- 3.6 Conclusion
- Acknowledgment
- References
- Chapter 4. Theoretical developments, instrumentation, and biological applications of surface-enhanced Raman spectroscopy
- Abstract
- 4.1 Introduction
- 4.2 Theory of surface-enhanced Raman spectroscopy
- 4.3 Instrumentation
- 4.4 Biological applications
- 4.5 Future outlook
- Acknowledgment
- References
- Chapter 5. Tip-enhanced Raman spectroscopy: fundamentals and applications
- Abstract
- 5.1 Introduction
- 5.2 Concept of tip-enhanced infrared spectroscopy
- 5.3 Instrumentation for tip-enhanced infrared spectroscopy
- 5.4 Advancement in tip-enhanced Raman spectroscopy
- 5.5 Chemical analysis by tip-enhanced Raman spectroscopy
- References
- Chapter 6. Stimulated Raman scattering microscopy: principles and life science applications
- Abstract
- 6.1 Introduction
- 6.2 Principle of stimulated Raman scattering microscopy
- 6.3 Life science applications
- 6.4 Conclusions
- References
- Chapter 7. Biomedical and clinical applications of Raman spectroscopy and multivariate chemometric methods
- Abstract
- 7.1 Introduction
- 7.2 Data acquisition and preprocessing
- 7.3 Multivariate analysis for pattern recognition
- 7.4 Future perspectives
- References
- Chapter 8. Raman spectroscopy and chemometrics: a potential method for life science applications
- Abstract
- 8.1 Introduction: the case for Raman fingerprinting in life sciences
- 8.2 Spectral complexity of biological molecules and samples
- 8.3 Chemometric modeling approach
- 8.4 Case study: Escherichia coli phenotypic responses to erythromycin exposure
- 8.5 Spectral processing
- 8.6 Unsupervised models
- 8.7 Supervised learning models
- 8.8 Molecular contributions
- 8.9 Using a model for predictions
- 8.10 Conclusions and moving forward
- References
- Chapter 9. Raman–gene integration provides a novel space of information to explore metabolism and gene function
- Abstract
- 9.1 Introduction
- 9.2 Key studies
- 9.3 Experimental approaches and considerations for Raman–gene integration
- 9.4 Conclusion
- References
- Chapter 10. Raman spectroscopic studies on mineralized tissues and skin appendages
- Abstract
- 10.1 Raman characteristic spectra for tissue characterization
- 10.2 Raman spectroscopic studies for tissue characterization
- 10.3 Raman spectroscopy in forensic science
- 10.4 Raman spectroscopy in biomedical applications
- 10.5 Conclusion
- References
- Chapter 11. Raman microscopy for early tumor detection
- Abstract
- 11.1 Introduction
- 11.2 Raman spectroscopy: fundamentals
- 11.3 Raman microscopy: principle and instrumentation
- 11.4 Application of Raman microscopy in cancer detection
- 11.5 Summary
- References
- Chapter 12. Raman spectroscopy for medical diagnostics: from in vitro biofluid assays to in vivo cancer detection
- Abstract
- 12.1 Introduction
- 12.2 Raman features and sampling
- 12.3 Instrumentation
- 12.4 Raman spectroscopic variations
- 12.5 Raman microspectroscopy
- 12.6 Tip-enhanced Raman scattering
- 12.7 Selective-sampling Raman microspectroscopy
- 12.8 Coherent anti-Stokes Raman spectroscopy
- 12.9 Stimulated Raman scattering
- 12.10 Surface-enhanced Raman spectroscopy
- 12.11 Spatially offset Raman spectroscopy
- 12.12 Transmission Raman spectroscopy
- 12.13 Data preprocessing
- 12.14 Smoothing and denoising
- 12.15 Background removal
- 12.16 Normalization
- 12.17 Chemometrics
- 12.18 Principal component analysis
- 12.19 Partial least squares
- 12.20 Classification and clustering models
- 12.21 In vivo and in vitro Raman diagnostics based on tissue analysis
- 12.22 Brain cancer
- 12.23 Colorectal cancer
- 12.24 Esophagus
- 12.25 Breast cancer
- 12.26 Lung cancer
- 12.27 Skin cancer
- 12.28 Biofluid diagnostic assays based on Raman spectroscopy
- 12.29 Diabetes and glucose level monitoring
- 12.30 Cancer diagnostics
- 12.31 Asthma
- 12.32 Inflammatory response
- 12.33 Coagulant and anticoagulant factors in human blood
- 12.34 Malaria
- 12.35 Conclusions
- References
- Chapter 13. Raman spectroscopy of soft tissues and body fluids
- Abstract
- 13.1 Introduction
- 13.2 Composition of soft tissues
- 13.3 Applications of Raman spectroscopy in soft tissues
- 13.4 Composition of body fluids
- 13.5 Applications of Raman spectroscopy based on body fluids
- 13.6 Conclusions
- References
- Chapter 14. Biochemistry of blood and application of Raman spectroscopy in studying blood cells, and membranes
- Abstract
- 14.1 Introduction
- 14.2 Biochemistry of blood
- 14.3 Application of Raman spectroscopy in studying blood cells
- 14.4 Application of Raman spectroscopy in studying blood cell membranes
- References
- Chapter 15. Raman spectroscopy of blood and blood components and chemometrics
- Abstract
- 15.1 Introduction
- 15.2 Raman spectroscopic studies of hemoglobin
- 15.3 Raman spectroscopy of intact red blood cells
- 15.4 White blood cells
- 15.5 Platelets
- 15.6 Raman spectroscopy of plasma
- 15.7 Serum
- 15.8 Whole blood
- 15.9 Raman spectroscopic in vivo analysis of blood
- 15.10 Conclusion
- References
- Chapter 16. Pathogen detection using Raman spectroscopy and surface-enhanced Raman scattering
- Abstract
- 16.1 Introduction
- 16.2 Food safety applications
- 16.3 Clinical applications
- 16.4 Conclusions
- Abbreviations
- References
- Chapter 17. Raman spectroscopy for analyzing renal stones
- Abstract
- 17.1 Introduction
- 17.2 Understanding human stone development and their compositions
- 17.3 Raman spectroscopy: principle and instrumentation
- 17.4 Applications of Raman spectroscopy in renal stones analysis
- 17.5 Identification of different types of renal stones
- 17.6 Calcium oxalate stones
- 17.7 Phosphate stones
- 17.8 Uric acid stones
- 17.9 Cystine stones
- 17.10 Mixed renal stones
- 17.11 Quantitative and nondestructive studies of renal stones
- 17.12 Quantitative analysis
- 17.13 Nondestructive layer analysis
- 17.14 Advanced Raman spectroscopy approaches for renal stone analysis
- 17.15 Raman spectroscopy in comparison to alternative approaches for the renal stones analysis
- 17.16 Clinical implementations, challenges, and future perspectives
- References
- Chapter 18. Applications of Raman spectroscopy in agriculture and chemometric assessment
- Abstract
- 18.1 Introduction
- 18.2 Raman spectroscopy and its various forms
- 18.3 Chemometrics and Raman spectroscopy
- 18.4 Applications of Raman spectroscopy in agricultural and food science
- 18.5 Conclusion
- References
- Chapter 19. Surface-enhanced Raman spectroscopy-based sensors as a futuristic analytical tool for environmental remediation and food safety
- Abstract
- 19.1 Introduction
- 19.2 Selection and design criteria of surface-enhanced Raman spectroscopy substrates
- 19.3 Surface-enhanced Raman spectroscopy sensor design and applications
- 19.4 Environmental pollution
- 19.5 Food safety
- 19.6 Conclusions
- References
- Chapter 20. Applications of surface-enhanced Raman spectroscopy in environmental detection
- Abstract
- 20.1 Introduction
- 20.2 Surface-enhanced Raman spectroscopy
- 20.3 Surface-enhanced Raman spectroscopy for plant hormones detection
- 20.4 Surface-enhanced Raman spectroscopy for melamine detection
- 20.5 Surface-enhanced Raman spectroscopy for DNA detection
- 20.6 Surface-enhanced Raman spectroscopy for virus detection
- 20.7 Surface-enhanced Raman spectroscopy for pesticide, fungicide, and insecticide detection
- 20.8 Surface-enhanced Raman spectroscopy for food analysis
- 20.9 Surface-enhanced Raman spectroscopy for antibiotic detection
- 20.10 Surface-enhanced Raman spectroscopy for trinitrotoluene detection
- 20.11 Conclusions
- Acknowledgment
- References
- Chapter 21. Surface-enhanced Raman spectroscopy–based sensors for environmental detection and pollutants monitoring
- Abstract
- 21.1 Introduction
- 21.2 Conventional and emerging types of water pollutants
- 21.3 Sources and consequences of water contaminants on the ecosystem and human health
- 21.4 Various contaminants, their origins, and their effects on the environment
- 21.5 Applications of surface-enhanced Raman spectroscopy to sensing platforms
- 21.6 Analytical techniques for pollutant detection in wastewater
- 21.7 Limitations
- 21.8 Surface-enhanced Raman spectroscopy–based nanosensors
- 21.9 Future aspects
- References
- Chapter 22. Raman spectroscopy: application to carbon-based nanomaterials
- Abstract
- 22.1 Introduction to Raman spectroscopy
- 22.2 Basic principles and instrumentation of Raman spectroscopy
- 22.3 Applications of Raman spectroscopy to carbon-based nanomaterials
- 22.4 Advanced techniques and potential developments
- References
- Chapter 23. Semiconductor-based surface-enhanced Raman spectroscopy
- Abstract
- 23.1 Introduction
- 23.2 Raman spectroscopy
- 23.3 Surface-enhanced Raman spectroscopy
- 23.4 Data processing
- 23.5 Semiconductor-based Raman scattering
- 23.6 Benefits of semiconductor-based substrate
- 23.7 Limitation of semiconductor-based substrate
- 23.8 Application of semiconductor surface-enhanced Raman spectroscopy substrate
- References
- Chapter 24. Surface-enhanced Raman spectroscopy technique for the estimation of thermal conductivity of thin film thermoelectric materials
- Abstract
- 24.1 Introduction
- 24.2 Thin film fabrication method
- 24.3 Estimating thermal conductivity of Bi2Te2.7Se0.3 thermoelectric thin films
- References
- Chapter 25. Hyphenated Raman spectroscopic systems: applications in biological, environmental, and materials sciences
- Abstract
- 25.1 Introduction
- 25.2 Environmental monitoring
- 25.3 Material characterization
- 25.4 Biomedical applications
- 25.5 Summary and future prospective
- References
- Chapter 26. Hyphenated Raman and laser spectroscopy for the characterization of microplastics in tissues
- Abstract
- 26.1 Introduction
- 26.2 Microplastics
- 26.3 Raman spectroscopy in the analysis of plastics
- 26.4 Laser-induced breakdown spectroscopy
- 26.5 Correlative analysis by using laser-induced breakdown spectroscopy and Raman spectroscopy
- 26.6 Laser-based spectroscopy and spectrometry for microplastics detection
- 26.7 Conclusion
- Acknowledgments
- References
- Chapter 27. Current and future prospect of Raman spectroscopy
- Abstract
- 27.1 Introduction
- 27.2 Types of Raman spectroscopy
- 27.3 Applications of Raman spectroscopy
- 27.4 Prospects of Raman spectroscopy
- 27.5 Conclusions
- References
- Index
- Edition: 1
- Published: March 17, 2025
- Imprint: Elsevier Science
- No. of pages: 498
- Language: English
- Paperback ISBN: 9780443218347
- eBook ISBN: 9780443218354
VS
Vivek Kumar Singh
Vivek Kumar Singh is an Associate Professor at the Department of Physics, University of Lucknow. He has more than 15 years of teaching and research experience at the university level. His current research interests include multi-spectroscopy studies of biological samples, plant samples, agricultural and food products, and nano-phosphor samples. He has published more than 90 international research papers and reviews articles. Dr Singh is a recipient of the prestigious “Raman Fellowship” for Post-Doctoral Research in Lawrence Berkeley National Laboratory, Berkley, USA.
Affiliations and expertise
Associate Professor, Department of Physics, University of Lucknow, Lucknow, IndiaRead Applied Raman Spectroscopy on ScienceDirect