Application of Sampling and Detection Methods in Agricultural Plant Biotechnology

1st Edition - July 30, 2022
Imprint: Woodhead Publ. & Cereals & Grains Assoc. Bookstore
Editors: Ray Shillito, Guomin Shan
Language: English
Paperback ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 2 9 3 - 0
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 9 9 2 9 4 - 7

Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This intern… Read more

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Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This international handbook, based on a series of workshops carried out for governments in collaboration with ILSI and Co-published in partnership with the Cereals & Grains Association, provides the technical and practical information needed to develop, validate and use detection methods. This useful resource provides readers with the tools necessary to carry out reliable sampling, detection and interpretation of data.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Foreword
Chapter 1. Introduction: genetically modified crops and their detection
Chapter 2. Seed purity testing and low-level presence
2.1. Background
2.2. Sampling
2.3. Detection methods and techniques
2.4. Protein-based detection methods
2.5. DNA-based detection methods
2.6. Managing costs in a detection laboratory
2.7. Bioassays
2.8. Low-level presence and purity
2.9. Data analysis and reporting
2.10. Conclusion
Chapter 3. Grain supply chain stewardship and testing—import and export
3.1. Introduction
3.2. Testing for import and export
3.3. Regulation and official controls
3.4. Analytical strategy
3.5. Stewardship
Chapter 4. Principles of nucleic acid-based detection methods
4.1. Introduction
4.2. DNA preparation and requirements
4.3. Nucleic acid hybridization
4.4. Amplification methods
4.5. Detection platforms
4.6. Next-generation sequencing
4.7. Closing remarks
Chapter 5. Method validation: DNA-based detection methods
5.1. Introduction
5.2. PCR validation
5.3. Data interpretation and sources of error
5.4. Reference materials
5.5. Assay transfer and proficiency testing
5.6. Summary
Chapter 6. Protein-based detection methods
6.1. Introduction
6.2. History
6.3. Principle
6.4. Application of immunoassays to GE crops
6.5. Production of antibodies
6.6. Assay design and operation
6.7. Conclusions
Chapter 7. Protein methods: antibody-based protein method validation and assay verification
7.1. Introduction
7.2. Quantitative ELISA method validation
7.3. Independent laboratory validations
7.4. Qualitative method validation
7.5. Assay verification
Chapter 8. Reference materials and working standards
8.1. Background
8.2. Uses of reference materials
8.3. What constitutes a reference material?
8.4. Reference materials in agricultural biotechnology
8.5. Practical limitations on reference material
8.6. Sources of reference materials
8.7. Reference material types
8.8. Specific considerations for using reference materials for quantification
8.9. International coordination and standardization
8.10. Future of reference material and the drivers
Chapter 9. Seed and grain sampling
9.1. Introduction
9.2. Sampling procedures
9.3. Probabilities of sampling
9.4. Testing of the laboratory sample
9.5. Acceptance sampling with qualitative testing
9.6. Acceptance sampling with quantitative testing
9.7. Acceptance sampling with qualitative testing on multiple subsamples
9.8. Conclusion
Chapter 10. Plant and field sampling
10.1. Introduction
10.2. Plant breeding
10.3. Seed and commercial crop testing
10.4. Direct testing in the field
10.5. Sampling for laboratory testing
10.6. Marking and labeling
10.7. Collecting the sample
10.8. Safety considerations
10.9. Specific procedures
10.10. Shipping samples
10.11. Summary
Chapter 11. Testing laboratory design and management
11.1. Introduction
11.2. Laboratory design
11.3. Laboratory workflow
11.4. Physical laboratory processes
11.5. Equipment management
11.6. Reagents
11.7. Assay quality control
11.8. Personnel management
11.9. Summary
Chapter 12. International standards and guidelines
12.1. Background and purpose of standards
12.2. Standards setting process
12.3. Standards organizations
12.4. Development of a standard
12.5. Standards relevant to GM detection
12.6. Conclusions
Chapter 13. Analytical strategy and interpretation of results
13.1. Introduction
13.2. Confirming the presence of intended trait
13.3. Confirming the absence of unintended traits
13.4. Interpreting results
Chapter 14. Detection methods for genome-edited crops
14.1. Introduction
14.2. What is genome editing—the key technologies
14.3. The drivers for detection methods
14.4. Detection method approaches
14.5. Differentiation of genome edits and spontaneous or induced untargeted mutations
14.6. Conclusions
Chapter 15. Future perspectives and challenges
15.1. Introduction
15.2. New GMO detection technologies
15.3. Closing remarks
Index

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Application of Sampling and Detection Methods in Agricultural Plant Biotechnology

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Ray Shillito

Guomin Shan

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