
Application of Sampling and Detection Methods in Agricultural Plant Biotechnology
- 1st Edition - July 30, 2022
- Imprint: Woodhead Publ. & Cereals & Grains Assoc. Bookstore
- Editors: Ray Shillito, Guomin Shan
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 2 9 3 - 0
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 9 2 9 4 - 7
Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This intern… Read more

Purchase options

Institutional subscription on ScienceDirect
Request a sales quoteApplication of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This international handbook, based on a series of workshops carried out for governments in collaboration with ILSI and Co-published in partnership with the Cereals & Grains Association, provides the technical and practical information needed to develop, validate and use detection methods. This useful resource provides readers with the tools necessary to carry out reliable sampling, detection and interpretation of data.
- Presents a review of the technologies and approaches used for sampling and detecting biotechnology products in seed, plants, grain, food and feed
- Serves as a GM detection manual for international regulators and government agencies, testing laboratories, and academic and industrial professionals
- Contains case studies, applications, literature reviews and coverage of recent developments
Academics and scientists working for agricultural biotechnology companies, within the seed and food and feed industries, GM test kit manufacturing companies, research and testing laboratories in the public and private sectors, and government agencies, as well as students studying related areas
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Foreword
- Chapter 1. Introduction: genetically modified crops and their detection
- Chapter 2. Seed purity testing and low-level presence
- 2.1. Background
- 2.2. Sampling
- 2.3. Detection methods and techniques
- 2.4. Protein-based detection methods
- 2.5. DNA-based detection methods
- 2.6. Managing costs in a detection laboratory
- 2.7. Bioassays
- 2.8. Low-level presence and purity
- 2.9. Data analysis and reporting
- 2.10. Conclusion
- Chapter 3. Grain supply chain stewardship and testing—import and export
- 3.1. Introduction
- 3.2. Testing for import and export
- 3.3. Regulation and official controls
- 3.4. Analytical strategy
- 3.5. Stewardship
- Chapter 4. Principles of nucleic acid-based detection methods
- 4.1. Introduction
- 4.2. DNA preparation and requirements
- 4.3. Nucleic acid hybridization
- 4.4. Amplification methods
- 4.5. Detection platforms
- 4.6. Next-generation sequencing
- 4.7. Closing remarks
- Chapter 5. Method validation: DNA-based detection methods
- 5.1. Introduction
- 5.2. PCR validation
- 5.3. Data interpretation and sources of error
- 5.4. Reference materials
- 5.5. Assay transfer and proficiency testing
- 5.6. Summary
- Chapter 6. Protein-based detection methods
- 6.1. Introduction
- 6.2. History
- 6.3. Principle
- 6.4. Application of immunoassays to GE crops
- 6.5. Production of antibodies
- 6.6. Assay design and operation
- 6.7. Conclusions
- Chapter 7. Protein methods: antibody-based protein method validation and assay verification
- 7.1. Introduction
- 7.2. Quantitative ELISA method validation
- 7.3. Independent laboratory validations
- 7.4. Qualitative method validation
- 7.5. Assay verification
- Chapter 8. Reference materials and working standards
- 8.1. Background
- 8.2. Uses of reference materials
- 8.3. What constitutes a reference material?
- 8.4. Reference materials in agricultural biotechnology
- 8.5. Practical limitations on reference material
- 8.6. Sources of reference materials
- 8.7. Reference material types
- 8.8. Specific considerations for using reference materials for quantification
- 8.9. International coordination and standardization
- 8.10. Future of reference material and the drivers
- Chapter 9. Seed and grain sampling
- 9.1. Introduction
- 9.2. Sampling procedures
- 9.3. Probabilities of sampling
- 9.4. Testing of the laboratory sample
- 9.5. Acceptance sampling with qualitative testing
- 9.6. Acceptance sampling with quantitative testing
- 9.7. Acceptance sampling with qualitative testing on multiple subsamples
- 9.8. Conclusion
- Chapter 10. Plant and field sampling
- 10.1. Introduction
- 10.2. Plant breeding
- 10.3. Seed and commercial crop testing
- 10.4. Direct testing in the field
- 10.5. Sampling for laboratory testing
- 10.6. Marking and labeling
- 10.7. Collecting the sample
- 10.8. Safety considerations
- 10.9. Specific procedures
- 10.10. Shipping samples
- 10.11. Summary
- Chapter 11. Testing laboratory design and management
- 11.1. Introduction
- 11.2. Laboratory design
- 11.3. Laboratory workflow
- 11.4. Physical laboratory processes
- 11.5. Equipment management
- 11.6. Reagents
- 11.7. Assay quality control
- 11.8. Personnel management
- 11.9. Summary
- Chapter 12. International standards and guidelines
- 12.1. Background and purpose of standards
- 12.2. Standards setting process
- 12.3. Standards organizations
- 12.4. Development of a standard
- 12.5. Standards relevant to GM detection
- 12.6. Conclusions
- Chapter 13. Analytical strategy and interpretation of results
- 13.1. Introduction
- 13.2. Confirming the presence of intended trait
- 13.3. Confirming the absence of unintended traits
- 13.4. Interpreting results
- Chapter 14. Detection methods for genome-edited crops
- 14.1. Introduction
- 14.2. What is genome editing—the key technologies
- 14.3. The drivers for detection methods
- 14.4. Detection method approaches
- 14.5. Differentiation of genome edits and spontaneous or induced untargeted mutations
- 14.6. Conclusions
- Chapter 15. Future perspectives and challenges
- 15.1. Introduction
- 15.2. New GMO detection technologies
- 15.3. Closing remarks
- Index
- Edition: 1
- Published: July 30, 2022
- Imprint: Woodhead Publ. & Cereals & Grains Assoc. Bookstore
- No. of pages: 304
- Language: English
- Paperback ISBN: 9780323992930
- eBook ISBN: 9780323992947
RS
Ray Shillito
Ray Shillito was a co-organizer of the ILSI International Food Biotechnology Committee (IFBiC)– American Association of Cereal Chemists International (AACCI) workshops from which the concept and much of the content of this book were derived and has presented the materials in the workshops given in many countries. As a BASF business support manager, he represents BASF (Seeds and Traits) in many trade associations and technical and science organizations, including CropLife, ISO, AOAC, Cereals & Grains Association (formerly AACC), and AEIC. He interacts internationally on standards setting in the area of analytical technology (with a focus on agricultural biotechnology), and he chairs the ISO committee TC34/SC16 (Biomarkers) and the Cereals & Grains Association Molecular Biomarkers for Grain Technical Committee.
Affiliations and expertise
BASF, USAGS
Guomin Shan
Guomin Shan received a bachelor’s degree in pesticide chemistry and a master’s degree in environmental toxicology from Beijing Agricultural University, China. In 1997, he received a PhD degree in entomology from Louisiana State University. After graduating, Guomin spent 4 years at the University of California, Davis as a postdoctoral fellow and a research scientist. He joined Dow AgroSciences LLC in 2001, where the focus of his career has been on transgenic traits and animal health product research and development. At Dow, he has served various leadership positions, including technical leader, science leader, seeds regulatory business partner, and global regulatory leader. Currently, he is the leader of the Predictive Safety Center in Corteva Crop Protection Regulatory Science.
Affiliations and expertise
Corteva Agriscience Crop Protection Regulatory Science, USARead Application of Sampling and Detection Methods in Agricultural Plant Biotechnology on ScienceDirect