Antimicrobial Peptides
- 1st Edition, Volume 663 - February 12, 2022
- Imprint: Academic Press
- Editor: Leslie Hicks
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 0 1 5 8 - 1
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 1 5 9 - 8
Antimicrobial Peptides, Volume 663 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Unifying the… Read more
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Request a sales quoteAntimicrobial Peptides, Volume 663 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on Unifying the classification of antimicrobial peptides in the Antimicrobial Peptide Database, Optimizing peptide library creation for PepSAVI-MS (RP libraries, etc.), Discovery of novel Antimicrobial peptides using BioProspecting, Screening for cysteine-stabilized scaffolds for developing protelytic-resistant AMPs, Exploring synergy and its role in antimicrobial peptide biology, Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens, and much more.
Other chapters cover Liquid chromatography-mass spectrometry-based analysis of naturally occurring neuropeptide diastereomers, Multiplexed Quantitative Neuropeptidomics via DiLeu Isobaric Tagging, In vitro evaluation of antibiotic resistance via proteomics, Molecular networking-based strategies in mass spectrometry, Development of Macrocyclic antimicrobial peptides and peptoids, and a host of other timely topics.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology serials
- Updated release includes the latest information on Antimicrobial Peptides
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Chapter One: Unifying the classification of antimicrobial peptides in the antimicrobial peptide database
- Abstract
- 1: Introduction
- 2: The problem of peptide classification
- 3: Classification platform
- 4: Unified classification of antimicrobial peptides
- 5: Relationship with source-dependent classifications of antimicrobial peptides
- 6: Concluding remarks
- Acknowledgment
- Chapter Two: Protocols for measuring the stability and cytotoxicity of cyclotides
- Abstract
- 1: Introduction
- 2: Structures and syntheses of cyclotides
- 3: Applications
- 4: Protocols
- 5: Analysis
- 6: Summary
- Acknowledgments
- Chapter Three: Creating optimized peptide libraries for AMP discovery via PepSAVI-MS
- Abstract
- 1: Introduction
- 2: Extraction of plant peptides
- 3: Peptide library creation
- 4: Bioassay
- 5: Quantitation via LC-MS
- 6: Statistical modeling and analysis
- 7: Summary
- Acknowledgments
- Chapter Four: Screening for cysteine-stabilized scaffolds for developing proteolytic-resistant AMPs
- Abstract
- 1: Introduction
- 2: Materials and equipment
- 3: Step-by-step method details
- 4: Safety consideration
- Acknowledgment
- Chapter Five: Exploring synergy and its role in antimicrobial peptide biology
- Abstract
- 1: Introduction
- 2: Considerations for activity testing in peptides
- 3: Synergy between AMPs
- 4: Synergy between AMPs and antibiotics
- 5: Synergy between AMPs and metal ions
- 6: A primer on Bliss and Loewe
- 7: Interpreting checkerboard assays
- 8: Checkerboard assay
- 9: A modified MIC assay for synergy testing
- 10: Modified MIC assay
- 11: Interpreting time-kill assays
- 12: Time-kill assay
- 13: Mechanistic studies
- 14: Proactive assessment of resistance
- 15: Overcoming the challenge of scale
- 16: Conclusions
- Acknowledgments
- Chapter Six: Colorimetric assays for the rapid and high-throughput screening of antimicrobial peptide activity against diverse bacterial pathogens
- Abstract
- 1: Introduction
- 2: AMP drug discovery
- 3: Using resazurin to assess antibacterial activity
- 4: Protocol: Bacterial inocula optimization
- 5: Step-by-step method details
- 6: Expected outcomes
- 7: Quantification and statistical analysis
- 8: Summary: Considerations when implementing high-throughput assays with optimized parameters
- Acknowledgments
- Chapter Seven: In silico prediction and mass spectrometric characterization of botanical antimicrobial peptides
- Abstract
- 1: Introduction
- 2: Materials
- 3: Protocol
- 4: Summary
- Acknowledgment
- Chapter Eight: Combined thermal and carboxypeptidase Y stability assays for probing the threaded fold of lasso peptides
- Abstract
- 1: Introduction
- 2: Concerning the thermal stability of lasso peptides
- 3: Combined heat and carboxypeptidase Y stability assays
- 4: Protocols
- 5: Analysis and data interpretation
- 6: Summary
- Chapter Nine: Evaluation of endogenous peptide stereochemistry using liquid chromatography-mass spectrometry-based spiking experiments
- Abstract
- 1: Introduction
- 2: Overview of the protocol
- 3: Before you begin—Determining putative positions of isomerization
- 4: Step-by-step methods
- 5: Conclusions
- Acknowledgments
- Chapter Ten: Multiplexed quantitative neuropeptidomics via DiLeu isobaric tagging
- Abstract
- 1: Introduction
- 2: Neuropeptide multiplexing via isobaric tagging
- 3: Protocol
- 4: Optimization of MS data acquisition
- 5: Applications of isobaric tagging in neuropeptidomics
- 6: Summary
- Acknowledgments
- Chapter Eleven: Revealing AMP mechanisms of action through resistance evolution and quantitative proteomics
- Abstract
- 1: Introduction
- 2: Materials
- 3: Methods
- 4: Summary
- Acknowledgments
- Chapter Twelve: Molecular networking-based strategies in mass spectrometry coupled with in silico dereplication of peptidic natural products and gene cluster analysis
- Abstract
- 1: Introduction
- 2: Materials
- 3: Analysis pipeline
- 4: Summary
- Chapter Thirteen: Methods for the design and characterization of peptide antibiotics
- Abstract
- 1: Introduction
- 2: Methods for the design of AMPs
- 3: Platforms for AMP screening
- 4: Conclusion
- Chapter Fourteen: Solid-phase synthesis of novel antimicrobial peptoids with α- and β-chiral side chains
- Abstract
- 1: Introduction
- 2: Materials
- 3: Equipment
- 4: Submonomer peptoid synthesis (manual)
- 5: Safety considerations and standards
- Chapter Fifteen: Molecular networking in infectious disease models
- Abstract
- 1: Introduction
- 2: Practical considerations
- 3: Protocol
- 4: Advantages
- 5: Limitations
- 6: Optimization and troubleshooting
- 7: Safety considerations and standards
- 8: Alternative methods/procedures
- 9: Summary
- Acknowledgments
- Edition: 1
- Volume: 663
- Published: February 12, 2022
- No. of pages (Hardback): 390
- No. of pages (eBook): 390
- Imprint: Academic Press
- Language: English
- Hardback ISBN: 9780323901581
- eBook ISBN: 9780323901598
LH
Leslie Hicks
Dr. Hicks received her B.S. in Chemistry at Marshall University, summa cum laude, and Ph.D. in Analytical Chemistry at the University of Illinois, Urbana-Champaign where she was the recipient of an NSF Graduate Research Fellowship. She was an Assistant Member and Principal Investigator at the Donald Danforth Plant Science Center and an adjunct professor in the Department of Biology at Washington University in St. Louis prior to assuming her current role as a professor in the Department of Chemistry at UNC.
Affiliations and expertise
Associate Professor, Department of Chemistry, University of North Carolina at Chapel Hill, USARead Antimicrobial Peptides on ScienceDirect