
Analysis of Marine Samples in Search of Bioactive Compounds
- 1st Edition, Volume 65 - August 26, 2014
- Imprint: Elsevier
- Editors: Teresa Rocha-Santos, Armando C. Duarte
- Language: English
- Hardback ISBN:9 7 8 - 0 - 4 4 4 - 6 3 3 5 9 - 0
- eBook ISBN:9 7 8 - 0 - 4 4 4 - 6 3 3 8 1 - 1
Seas and oceans offer a wide range of temperature, pressure, light and chemical conditions thus allowing a wide diversity of marine organisms from shallow coastal waters to the de… Read more

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Request a sales quoteSeas and oceans offer a wide range of temperature, pressure, light and chemical conditions thus allowing a wide diversity of marine organisms from shallow coastal waters to the deep ocean. These resources can be used to obtain new products and develop services, and in turn help to provide solutions to the challenges that affect our planet, including offering a sustainable supply of food and energy, new industrial materials and processes, new bioactive compounds, and new health treatments. Marine compounds have been identified as having antibacterial, anticoagulant, antifungal, antimalarial, antiprotozoal, antituberculosis, and antiviral activities. The major sources of these bioactive compounds are marine sponges, coelenterates, and microorganisms, followed by algae, echinoderms, tunicates, molluscs, and bryozoans.
The discovery of bioactive compounds from marine samples is a hot topic considering the current need for sustainable use of marine resources. This book is a comprehensive overview of the analytical techniques employed in the discovery and characterization of bioactive compounds isolated from (all possible) marine samples and gives future perspectives of analytical methodologies. This overview includes an assessment of the sampling and preparation of extracts, the separation and isolation of bioactive compounds, their structural characterization and the application of bioassays in the discovery of bioactive compounds.
- Comprehensive coverage of analytical techniques and applications
- Clear diagrams to adequately support important topics
- Real examples of applications of analytical techniques in the search for new bioactive compounds
Marine scientists, analytical chemists, organic chemists, biologists, food scientists, and pharmaceutical scientists
- Contributors to Volume 65
- Series Editor's Preface
- Preface
- Chapter 1: Introduction to the Analysis of Bioactive Compounds in Marine Samples
- Abstract
- 1.1. Importance of bioactive compounds
- 1.2. Sources of bioactive compounds
- 1.3. Classes of bioactive compounds
- 1.4. General approaches for screening bioactive compounds
- Chapter 2: Prospection, Collection, and Preservation of Marine Samples
- Abstract
- 2.1. Introduction
- 2.2. Bioprospecting in extreme environments
- 2.3. Collection of marine samples
- 2.4. Sustainable production of bioactive compounds
- 2.5. Preservation of marine samples
- 2.6. Final considerations
- Acknowledgements
- Chapter 3: Classical Methodologies for Preparation of Extracts and Fractions
- Abstract
- 3.1. Sample preparation of bioactive compounds from the marine environment
- 3.2. Final considerations
- Chapter 4: Green Analytical Methodologies for Preparation of Extracts and Analysis of Bioactive Compounds
- Abstract
- 4.1. Green extraction techniques to obtain bioactive compounds
- 4.2. Direct analysis of untreated samples to obtain bioactive compounds
- Chapter 5: Bioassays for Bioactivity Screening
- Abstract
- 5.1. Introduction
- 5.2. Screening models
- 5.3. Toxicity evaluation
- 5.4. Use of animals
- 5.5. Clinical trials
- 5.6. Conclusions and future perspectives
- Acknowledgements
- Chapter 6: Vibrational Spectroscopy for Structural Characterization of Bioactive Compounds
- Abstract
- 6.1. Vibrational spectroscopy
- 6.2. Introduction to infrared (IR) and Raman spectroscopy
- 6.3. Selection rules in vibrational spectroscopy
- 6.4. Structure characterization by vibrational spectroscopy
- 6.5. Conclusion
- Acknowledgements
- Chapter 7: Nuclear Magnetic Resonance Spectroscopy for Structural Characterization of Bioactive Compounds
- Abstract
- 7.1. Introduction
- 7.2. Proton resonances assignment
- 7.3. Establishment of carbon skeleton
- 7.4. Stereochemistry
- 7.5. Hyphenated NMR techniques
- 7.6. Quantitative NMR
- 7.7. Combined NMR techniques and different families of compounds
- 7.8. Revised structures
- Acknowledgements
- Chapter 8: Mass Spectrometry for Determination of Bioactive Compounds
- Abstract
- 8.1. Introduction
- 8.2. Mass spectrometry (MS)
- 8.3. Tandem mass spectrometry (MSn)
- 8.4. Liquid chromatography–mass spectrometry (LC-MS)
- 8.5. Application of MS
- 8.6. Peptides
- 8.7. Alkaloids
- 8.8. Lipids
- 8.9. Conclusion
- Acknowledgements
- Chapter 9: Chromatography Coupled to Various Detectors as a Tool for Separation and Determination of Bioactive Compounds
- Abstract
- 9.1. Introduction
- 9.2. One-Dimensional chromatography for bioactive compound analysis
- 9.3. Multidimensional chromatography for bioactive compounds discovery
- 9.4. Concluding remarks
- Acknowledgments
- Chapter 10: Online Combination of Bioassays with Chemical and Structural Characterization for Detection of Bioactive Compounds
- Abstract
- 10.1. Introduction
- 10.2. High-throughput screening methods
- 10.3. High-resolution-based screening methods
- 10.4. Conclusions and perspectives
- Acknowledgments
- Index
- Edition: 1
- Volume: 65
- Published: August 26, 2014
- Imprint: Elsevier
- No. of pages: 312
- Language: English
- Hardback ISBN: 9780444633590
- eBook ISBN: 9780444633811
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Teresa Rocha-Santos
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