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Advances in Plant Tissue Culture

Current Developments and Future Trends

1st Edition - May 28, 2022

Editors: Avinash Chandra Rai, Ajay Kumar, Arpan Modi, Major Singh

Paperback ISBN:

9 7 8 - 0 - 3 2 3 - 9 0 7 9 5 - 8

eBook ISBN:

9 7 8 - 0 - 3 2 3 - 9 9 8 1 9 - 2

Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and… Read more

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Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry.

This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation.

Cover image
Title page
Table of Contents
Copyright
Contributors
Chapter 1: A general introduction to and background of plant tissue culture: Past, current, and future aspects
Abstract
1: Introduction
2: Milestones
3: Direct and indirect organogenesis
4: Somatic embryogenesis
5: Plant tissue culture for trait improvement
6: Plant tissue culture for plant production
7: Conclusion and future trends
References
Chapter 2: Micropropagation for multiplication of disease-free and genetically uniform sugarcane plantlets
Abstract
1: Introduction
2: History of plant tissue culture
3: Micropropagation
4: Micropropagation studies in sugarcane
5: Quality assurance for disease-free and genetically uniform seed cane
6: Advantages of tissue culture-raised sugarcane
7: Constraints of in vitro propagation
8: Conclusions
References
Chapter 3: Role of plant tissue culture medium components
Abstract
Acknowledgments
1: Introduction
2: Basic composition of culture medium
3: Additional supplements to culture medium
4: Liquid culture medium
5: Sterilization of culture medium
6: Photoautotrophic culture medium
7: Automation of culture medium preparation
8: Utilization of nanoparticles in culture medium
9: Conclusion and prospects
References
Chapter 4: The journey and new breakthroughs of plant growth regulators in tissue culture
Abstract
1: Introduction
2: Auxin
3: Cytokinin
4: Gibberellic acid
5: Abscisic acid
6: Brassinosteroid
7: Jasmonic acid
8: Salicylic acid
9: Strigolactones
10: Phloroglucinol
11: Other PGRs and tissue culture of recalcitrant plants
12: Conclusion and future prospective
References
Chapter 5: Plant tissue culture: Somatic embryogenesis and organogenesis
Abstract
1: Introduction
2: Somatic embryogenesis
3: Organogenesis
References
Chapter 6: Genetic transformation via plant tissue culture techniques: Current and future approaches
Abstract
1: The importance of the genetic transformation technique for research and production of superior plants
2: History of plant genetic transformation technique development
3: Genetic transformation via plant tissue culture technique
4: Important genes are widely transferred to plants
5: Perspective on genetic transformation as an excellent tool in biotechnology
References
Chapter 7: Anther culture for haploid plant production
Abstract
1: Introduction
2: History of anther culture
3: A generalized method for haploid production through anther culture
4: Factors affecting anther culture
5: Production of doubled haploid plant
6: Identification of haploid plants
7: Application of anther culture in crop improvements
8: Progress in the haploid production through anther culture
9: Conclusion and prospects
References
Chapter 8: Morpho-anatomical characterization of in vitro regenerated plants
Abstract
Acknowledgments
1: Introduction
2: Essential oil rose
3: Narrow-leaved lavender and lavandin
4: Garden Canna
5: Garden chrysanthemum
6: Clematis
7: Common fig
8: Persimmon
9: Peach
10: Conclusion and perspective
References
Chapter 9: Plant tissue culture targeting germplasm conservation
Abstract
1: Introduction
2: Short- and medium-term conservation through slow growth storage
3: Long-term conservation by cryopreservation
4: Concluding remarks
References
Chapter 10: Somatic embryogenesis in medicinal plants
Abstract
Acknowledgments
1: Introduction
2: Somatic embryogenesis
3: Modes of somatic embryogenesis
4: Somatic embryogenesis (SE) and plant recovery
5: Advantages of somatic embryogenesis
6: Disadvantages of somatic embryogenesis
7: Factors affecting somatic embryogenesis
8: Molecular aspects of somatic embryogenesis
9: Uses of somatic embryogenesis in plant improvement
10: Conclusion
References
Chapter 11: Embryo rescue: A potential tool for improvement of economically important crops
Abstract
Acknowledgment
1: Introduction
2: History of embryo rescue
3: Culture medium for embryo rescue
4: Manipulation of embryo for culture
5: Embryo culture technique
6: Applications of embryo culture
7: Future prospects and conclusion
References
Chapter 12: Strategies to overcome explant recalcitrance under in vitro conditions
Abstract
1: Introduction
2: Factors contributing to recalcitrance
3: Molecular basis of recalcitrance
4: Case studies
5: Conclusion
References
Further reading
Chapter 13: Improving crops through transgenic breeding—Technological advances and prospects
Abstract
1: Introduction
2: Plant tissue culture techniques of relevance to GT
3: Genetic transformation methods and the need for new developments
4: Assessment of different methods of genetic transformation
5: Factors affecting transformation efficiency
6: New methods of genetic transformation
7: Conclusions and prospects
References
Chapter 14: Meristem culture: A potential technique for in vitro virus-free plants production in vegetatively propagated crops
Abstract
Acknowledgment
1: Introduction
2: Transmission of plant viruses
3: Need for meristem culture
4: Meristem isolation from infected plants
5: Culture media for meristem culture
6: Eradication of plant viruses through meristem culture
7: Future prospects
8: Conclusion
References
Chapter 15: Plant tissue culture in tree species
Abstract
1: Introduction
2: Tissue culture on tree species
3: Regeneration through organogenesis
4: Regeneration through somatic embryogenesis
5: Study of genetic fidelity to improve plants’ quality
6: Conclusion
References
Chapter 16: The role of plant tissue culture in pharmaceuticals and secondary metabolites production
Abstract
1: Introduction
2: Secondary metabolites
3: Role of metabolic engineering in secondary metabolite production
4: Elicitors
5: Transgenic plants
6: Conclusion and future perspective
References
Chapter 17: Tissue culture: A perpetual source for the conservation of medicinally important endangered plant species
Abstract
1: Introduction
2: The importance of tissue culture of medicinally important plants
3: Established tissue cultures of some medicinal plants
4: The role of tissue culture in genetic engineering of medicinally endangered plants
5: Genetic transformation
6: Production of secondary metabolites from medicinal plants
References
Chapter 18: Phytochemical studies in the field of plant tissue culture
Abstract
1: Introduction
2: The history of phytochemicals in medicinal plant research
3: Phytochemical and tissue culture
4: Secondary metabolites
5: Conclusion
References
Further reading
Index

Avinash Chandra Rai

Dr. Avinash Chandra Rai is currently working as a visiting scientist at Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel. Dr. Rai has been completed his Ph.D. degree in Biotechnology from Banaras Hindu University, Uttar Pradesh, India. He is currently serving as a visiting Scientist in Institute of Plant Sciences, Agricultural Research Organization-The Volcani Centre, Israel. He has more than thirteen years of research experience, particularly in the area of plant molecular biology and plant physiology. His research interest is in deciphering the insights of plant abiotic stresses with the applications of molecular and physiological tools. He also has a keen interest in emerging environmental and physiological issues of crop plants. He is currently working to see the effect of auxin and ethylene in the process of abscission in mango and find out the role of essential gene involved in the pathways mechanism in abscission of fruit. Dr. Rai has more than seventeen research articles and book chapters in journals of national and international repute to his credit. He is a lifetime member of the Biotech Research Society of India (BRSI) and Society of vegetable Sciences, India. He is also the recipient of Young Scientist Associate award from Bioved Research Society, Allahabad, India in 2016. He has also actively participated in many national and international conferences, symposia and workshops related to his research field in India and abroad.

Ajay Kumar

Dr. Ajay Kumar is a visiting scientist at the Department of Postharvest Science, Agriculture Research Organization, Volcani Center, Rishon LeZion, Israel. He has published extensively in leading International and National journals or books. He has wide area of research experience, especially in the field of Plant-Microbe Interactions, Microbial biocontrol, and Endophytes related with the medicinal plants, Postharvest management of fruits. Dr. Kumar has published more than 165 research articles including review papers/book chapters/ books in the leading international journals. He is serving as special issue editors in some journals like “Frontiers in Microbiology, Microorganism” etc. In addition also acting as reviewer in some leading journals like Frontiers group, PLOS ONE, Agriculture, Ecosystem and Environment, International Journal of Molecular Sciences, Biological Control, Plant Biochemistry and Biotechnology, 3Biotech etc. Dr, Kumar has edited several books with leading publishers including Elsevier, Springer, and Taylor and Francis.

Affiliations and expertise

Agricultural Research Organization

Arpan Modi

Dr. Arpan Modi is currently working as a visiting scientist at Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel. Dr. Modi has completed his doctoral degree in Plant Biotechnology from Anand Agricultural University, Gujarat, India. Since 2009, with more than 11 years of vast research experience, he has developed skills for various operations and troubleshooting in plant tissue culture. His research interest focuses mainly on the use of an interdisciplinary approach for the betterment of the plant life cycle. He has been through the area of plant biochemistry, plant biotechnology, plant molecular biology and nanotechnology. Currently, he has involved in the development of the protocol for the micropropagation of Ficus microcarpa and genome editing in the same for quality improvement. Dr. Modi has 13 research articles and book chapters in journals of national and international repute to his credit. He is a lifetime member of the Society for Sugar Research and Promotion, Uttar Pradesh, India. He has also actively participated in many national and international conferences, symposia and workshops related to his research field in India and abroad.

Affiliations and expertise

Visiting Scientist, Agriculture Research Organization, The Volcani Center, Bet Dagan, Israel

Major Singh

Dr. Major Singh, presently works as Director of ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, from 2017 to date and he served as Principal Scientist and Head, Division of Crop Improvement, Indian Institute of Vegetable Research, Varanasi, from the year 2010 to 2015; Scientist, ICAR Research Complex for NEH Region, Shillong, 1986-96; Sr Scientist and Principal Scientist, IIVR, Varanasi, 1996-2010. Project Coordinator and also act as Coordinator of All India Coordinated Research Project on Vegetable Crops, IIVR, and Varanasi 2011-16. Dr. Singh has published more than 200 Research articles in the various national and International Journal. Moreover he had been awarded by various scientific societies in his 35 years of research experience like Outstanding Multidisciplinary Team Research Award of ICAR for Biennium 2003-04; Rajeev Gandhi National Award, 2009-10; Dr. Harbhajan Singh Award, 2004, ISVS, Varanasi; Dr. Harbhajan Singh Award, 2005, ISVS, Varanasi.

Affiliations and expertise

Director of ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, India