Contributors to Volume
Preface
Renato Dulbecco
Contents of Previous Volumes
Terminal Differentiation in Cultured Human Epidermal Cells
I. The Process of Terminal Differentiation in Human Epidermis
II. The Process of Terminal Differentiation in Cultured Human Epidermal Cells
III. Extracellular Regulators of Terminal Differentiation
IV. The Structures of the Proteins and the Genes Expressed in Epidermis: A Focus on the Keratins
References
Microvascular Endothelial Cells: Isolation, Identification, and Cultivation
I. Introduction 36
II. Isolation and Cultivation of Microvascular Endothelial Cells from Different Vascular Beds
III. Identification of Microvascular Endothelial Cells
IV. Special Techniques and Culture Ingredients Useful in the Isolation and Culture of Microvessel Endothelial Cells
References
The Production of Artemisinin in Artemisia annua L Tissue Cultures
I. Introduction
II. Artemisia Plants
III. Artemisia Tissue Cultures
IV. Summary
References
Plant Cell Suspensions for Screening and Studying the Mode of Action of Plant Growth Retardants
I. General Remarks on the Application of Cell Cultures in Plant Biotechnology
II. Cell Suspension Cultures in Environmental and Agrochemical Research
III. Practical Use of Plant Growth Retardants in Agriculture
IV. Regulation of the Meristematic Activity in Plants by Growth Retardants
V. Heterotrophically Cultivated Cell Suspensions for Imitating the Response of Whole Plants to Growth Retardants
VI. Designing and Establishing a Micromethod for Large-Scale Screening of Growth Retardants
VII. Elucidating the Mode of Action of Growth Retardants
VIII. Summary
IX. Appendix: Abbreviations and Nomenclature of Growth Retardants
References
Cultures of Mammary Epithelial Cells: Extracellular Matrix and Functional Differentiation
I. Introduction
II. Mouse Mammary Epithelial Cells
III. Human Mammary Epithelial Cells
IV. Possible Mechanism of ECM Action
V. Cell Culture Procedures
References
Cell Culture for Testing Anticancer Compounds
I. Introduction
II. Assays of Cell Survival and/or Structural Integrity
III. Assays of Metabolic Integrity
IV. Assays of Functional Integrity/Reproductive Capability
V. Other Assays with Potential for Application to Anticancer Drug Testing
VI. Summary and Conclusions
References
Asbestos and Transfection
I. Introduction
II. Asbestos
III. Asbestos and Disease
IV. Effects of Asbestos in Laboratory Rodents
V. Effects of Asbestos on Organ Cultures
VI. Asbestos-Induced Hemolysis
VII. Toxicity of Asbestos for Cell Cultures
VIII. Uptake of Asbestos by Cells
IX. Transformation of Cultured Cells by Asbestos
X. Asbestos as a Mutagen
XI. Association of Asbestos with Chromosomes and Nucleic Acids
XII. "Insoluble" Facilitators of Transfection
XIII. Roles for Asbestos in Carcinogenesis
XIV. Summary
Note Added in Proof
References
Cell Injury, Differentiation, and Regeneration in Explant, Organ, and Cell Culture Models
I. Introduction and Hypothesis
II. Culture Methods
III. Organ-Specific Cell Injury in Vitro
References
In vitro Genetic Manipulation of Cereals and Grasses
I. Introduction
II. Tissue, Cell, and Protoplast Culture
III. Vectorless Gene Transfer
IV. Agrobacterium-Mediated Gene Transfer
V. Summary
References
Note Added in Proof
Index