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Advances in Biomolecular EPR
- 1st Edition, Volume 666 - April 22, 2022
- Editor: R. David Britt
- Language: English
- Hardback ISBN:9 7 8 - 0 - 3 2 3 - 9 9 9 7 4 - 8
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 8 3 0 5 - 1
Advances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topi… Read more
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Request a sales quoteAdvances in Biomolecular EPR, Volume 666 in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics including Magnetic Resonance Characterization of Physiologically Important Metal Ion Binding Sites in the Prion and Related Proteins, The catalytic role of metal-radical/protein-based radicals in heme enzymes, Rigid Cu2+-based spin labels for the study of higher-order DNA G-quadruplex structures, Orthogonal spin labeling and membrane proteins: increasing the information content and going towards in cell applications, Spectroscopic investigation of mono- and di-Mn-containing centers in biochemistry with an emphasis on application of paramagnetic resonance techniques, and more.
Additional chapters cover In Vivo pO2 Imaging of Tumors: Oxymetry with Very Low-Frequency Electron Paramagnetic Resonance, an Update, EPR contributions to understanding molybdenum-containing enzymes, EPR spectroscopy of Type I reaction centers, Characterization of a substrate-derived radical in the NosN reaction during the biosynthesis of nosiheptide, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in Methods in Enzymology series
- Includes the latest information on Advances in Biomolecular EPR
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- Cover image
- Title page
- Table of Contents
- Copyright
- Contributors
- Chapter One: Advances in rapid scan EPR spectroscopy
- Abstract
- 1: Over-view of the rapid-scan experiment
- 2: Recent hardware developments
- 3: Algorithms for deconvolution of rapid scans
- 4: Examples of applications
- 5: Guidance on parameter selection for field-swept rapid scan
- 6: Summary
- Acknowledgments
- References
- Chapter Two: Resolution and characterization of confinement- and temperature-dependent dynamics in solvent phases that surround proteins in frozen aqueous solution by using spin-probe EPR spectroscopy
- Abstract
- 1: Introduction
- 2: Preparation of protein–mesodomain system in frozen solution
- 3: Measurement of temperature-dependent EPR spectra
- 4: Simulation of temperature-dependent EPR spectra
- 5: Protein environment in the frozen aqueous solutions
- 6: Conclusions and future directions
- Acknowledgments
- References
- Chapter Three: Site directed spin labeling to elucidating the mechanism of the cyanobacterial circadian clock
- Abstract
- 1: Introduction
- 2: Experimental considerations
- 3: Spin labeling of protein of interest
- 4: EPR sample preparation and data acquisition
- 5: Concluding remarks
- Acknowledgments
- References
- Chapter Four: Orthogonal spin labeling and pulsed dipolar spectroscopy for protein studies
- Abstract
- 1: Introduction
- 2: Properties of four common orthogonal labels
- 3: Pulsed dipolar spectroscopy
- 4: PDS measurements with orthogonal labels
- 5: Orthogonality and channel crosstalk
- 6: Applications
- 7: Outlook
- References
- Chapter Five: Tracking protein domain movements by EPR distance determination and multilateration
- Abstract
- 1: Introduction
- 2: Experimental guidelines
- References
- Chapter Six: Integrative ensemble modeling of proteins and their complexes with distance distribution restraints
- Abstract
- 1: Introduction
- 2: Concept of RigiFlex modeling of ensemble structures
- 3: Modeling and analysis pipelines
- 4: Application examples
- 5: Conclusion
- Acknowledgments
- References
- Chapter Seven: Light-induced pulsed dipolar EPR spectroscopy for distance and orientation analysis
- Abstract
- 1: Introduction
- 2: The theory of pulsed dipolar spectroscopy
- 3: LiPDS methods
- 4: Examples of biological applications for LiPDS
- 5: Choice of LiPDS technique
- 6: Methods of illumination
- 7: Choice of chromophore
- 8: Conclusions
- Acknowledgments
- References
- Chapter Eight: Controlling and exploiting intrinsic unpaired electrons in metalloproteins
- Abstract
- 1: Introduction
- 2: First things first: The protein
- 3: Determining reduction potentials and “directing” electrons biochemically
- 4: Using pulsed EPR to gain detailed insight into unpaired electrons
- 5: Conclusions and outlook
- Acknowledgments
- References
- Chapter Nine: EPR of copper centers in the prion protein
- Abstract
- 1: Introduction
- 2: Sample preparation
- 3: Continuous wave (CW) EPR
- 4: Electron spin echo envelope modulation (ESEEM)
- 5: Double electron-electron resonance (DEER) EPR
- 6: Concluding remarks
- Acknowledgments
- References
- Chapter Ten: Paramagnetic resonance investigation of mono- and di-manganese-containing systems in biochemistry
- Abstract
- 1: Introduction
- 2: Overview of EPR and related techniques
- 3: Overview of EPR and related techniques applied to biological Mn-containing systems
- 4: Summary
- Acknowledgments
- References
- Chapter Eleven: Application of EPR and related methods to molybdenum-containing enzymes
- Abstract
- 1: Introduction
- 2: Xanthine oxidase family enzymes
- 3: Sulfite oxidase family enzymes
- 4: DMSO reductase family enzymes
- 5: Summary
- Acknowledgments
- References
- Chapter Twelve: EPR of Type I photosynthetic reaction centers
- Abstract
- 1: Introduction
- 2: Continuous wave (CW) EPR spectroscopy of Type I reaction centers
- 3: Time-resolved EPR of Type I reaction centers
- 4: Summary
- Acknowledgments
- References
- Chapter Thirteen: Experimental guidelines for trapping paramagnetic reaction intermediates in radical S-adenosylmethionine enzymes
- Abstract
- 1: Introduction
- 2: Overview of changes to original SuiB expression and purification protocol
- 3: Protocol
- 4: Conclusion
- Acknowledgments
- References
- Chapter Fourteen: Using peptide substrate analogs to characterize a radical intermediate in NosN catalysis
- Abstract
- 1: Introduction
- 2: Synthesis of NosN substrate surrogates and their deuterated analogs
- 3: EPR sample preparation and data collection
- 4: EPR data simulation
- 5: Conclusions
- Acknowledgments
- References
- No. of pages: 500
- Language: English
- Edition: 1
- Volume: 666
- Published: April 22, 2022
- Imprint: Academic Press
- Hardback ISBN: 9780323999748
- eBook ISBN: 9780323983051
RB
R. David Britt
R. David Britt is the Winston Ko Chair and Distinguished Professor of Chemistry at the University of California, Davis. Prof. Britt uses electron paramagnetic resonance (EPR) spectroscopy to study metalloenzymes and enzymes containing organic radicals in their active sites.Britt is the recipient of multiple awards for his research, including the Bioinorganic Chemistry Award in 2019 and the Bruker Prize in 2015 from the Royal Society of Chemistry. He has received a Gold Medal from the International EPR Society (2014), and the Zavoisky Award from the Kazan Scientific Center of the Russian Academy of Sciences (2018).He is a Fellow of the American Association for the Advancement of Science and of the Royal Society of Chemistry.
Affiliations and expertise
Department of Chemistry, University of California, DavisRead Advances in Biomolecular EPR on ScienceDirect