A Complete Guidebook on Biofilm Study

1st Edition - June 22, 2022

Editor: Dijendra N. Roy

Paperback ISBN:

9 7 8 - 0 - 3 2 3 - 8 8 4 8 0 - 8

eBook ISBN:

9 7 8 - 0 - 3 2 3 - 8 8 4 8 1 - 5

A Complete Guidebook on Biofilm Study has emphasized the biofilm-related issues in the present context related to research and development. For this purpose, experimental design… Read more

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A Complete Guidebook on Biofilm Study has emphasized the biofilm-related issues in the present context related to research and development. For this purpose, experimental design and relevant experimental protocols for the biofilm studies have been highlighted here. In addition to that, inhibitors from natural or synthetic sources against microbial biofilm development have been addressed. This approach has been further substantiated by bioinformatics as well as nanotechnology-based reports. Both, the image processing related to biofilm study and the characters of substratum associated with biofilm development have also been included for a better understanding of the beginners in this field. Further, how biofilm helps and/or hampers in food processing and waste management system, that discussion has been considered in this book. Similarly, human benefits from biofilm and reverse of it have also been included considering host-pathogen interaction, immunity aspects, and others.

Cover Image
Title Page
Copyright
Dedication
Table of Contents
Contributors
Editor's biography
Preface
Acknowledgments
Chapter 1 Biofilm: Design of experiments and relevant protocols
1.1 Introduction
1.2 Biofilm: Formation, development, and its consequences
1.3 Requirement of biofilm in microbial community
1.4 Purpose of various biofilm experiments
1.5 Reports on model microbes for biofilm experiments
1.6 Standard protocols for various biofilm-associated experiments
1.7 Scope for modification of experimental protocols
1.8 Conclusion
References
Chapter 2 Physiology of biofilm produced by various microbes
2.1 Introduction
2.2 Physiological structure of bacterial biofilm
2.3 Physiological structure of microbial biofilm
2.4 Conclusion
References
Chapter 3 Genetic regulations of microbial biofilm
3.1 Introduction
3.2 Regulation of biofilm formation in bacteria
3.3 Genetic regulation in fungal biofilms
3.4 Biofilm formation by protists
3.5 Effect of antimicrobials on biofilms and current strategies to eradicate biofilms
3.6 Conclusion
References
Chapter 4 Combating biofilm of ESKAPE pathogens from ancient plant-based therapy to modern nanotechnological combinations
4.1 Introduction
4.2 Antimicrobial resistance in ESKAPE
4.3 Major issues linked to antimicrobial resistance in ESKAPE pathogens
4.4 Role of biofilm in pathogenicity and drug resistance
4.5 Biofilm as a drug target
4.6 Traditional medicinal system in controlling biofilms
4.7 Medicinal plants in interference of bacterial quorum sensing: Mechanism of action
4.8 Nanomaterials and their mechanism of action
4.9 Alternative approaches in the pipeline that works without quitting older antibiotics
4.10 Conclusion
4.11 Future perspective
Acknowledgments
References
Chapter 5 Application of natural products against fungal biofilm formation
5.1 Introduction
5.2 Fungal biofilms in disease development
5.3 Antifungal drug targets and resistance mechanisms in biofilms
5.4 Common genes involved in fungal biofilm development
5.5 Role of natural product in fungal biofilm control
5.6 Antibiofilm phytocompounds and their mode of action
5.7 New approaches to improve antifungal efficacy of natural products against fungal biofilms
5.8 In vivo studies on natural product application in combination with antifungal drugs
5.9 Other applications of antibiofilm agent
5.10 Challenges
5.11 Conclusion and future perspective
Acknowledgments
Conflict of interest
References
Chapter 6 Application of synthetic products against pathogenic fungal biofilm development with special reference to combinational approaches
6.1 Introduction
6.2 Problem and challenges associated with current antifungal therapy
6.3 Combinational therapy
6.4 Mechanism of drug interaction
6.5 Mode of action of combinational treatment
6.6 Conclusion and future prospects
Conflict of interest
References
Chapter 7 Metal nanoparticles against growth of microbial biofilm
7.1 Microbes and biofilm
7.2 Mechanism of biofilm formation
7.3 Biofilm and multiple drug resistance (MDR)
7.4 Metal nanoparticles against biofilm growth
7.5 Green synthesized MNPs and their antibiofilm action
7.6 Future improvement of MNPs-mediated biofilm inhibition
7.7 Conclusion
References
Chapter 8 Role of biofilm in waste management system
8.1 Introduction
8.2 Classification of biological treatment systems
8.3 Biofilm in liquid waste treatment
8.4 Biofilm in solid waste treatment
8.5 Biofilm in food waste treatment
8.6 Future scope on biofilm in waste management system
References
Chapter 9 Control of Biofilm Formation during Food Processing
9.1 Introduction
9.2 Factors affecting biofilm formation during food processing
9.3 Formation of biofilms during food processing
9.4 Methods to control biofilm formation during food processing
9.5 Conclusion
Acknowledgments
References
Chapter 10 Role of biofilm in host–pathogen interaction
10.1 Introduction
10.2 Biofilm in immune evasion strategies
10.3 Diseases caused by biofilms
10.4 Biofilm: Functions as virulent
10.5 Defense mechanism of human host against biofilm-associated infections
10.6 Host responses to biofilm
10.7 Existing therapies
References
Chapter 11 Antibiotic resistance in biofilm-forming pathogenic Coliforms & the role of membrane proteins
11.1 Introduction
11.2 Biofilm formation by pathogenic coliforms
11.3 Mechanism of drug resistance in biofilm forming coliforms
11.4 Case study on bacterial biofilm assessment
11.5 Role of membrane proteins in biofilm formation
11.6 Conclusion
List of abbreviations/symbols
References
Chapter 12 Image processing of biofilms and its applications
12.1 Introduction
12.2 Challenges in image processing of biofilms and their solutions
12.3 Image processing tools
12.4 Future scope of biofilm analysis techniques
References
Chapter 13 Modification of material surface to regulate biofilm formation
13.1 Introduction
13.2 Biofilm-associated biofouling in metals and alloys
13.3 Role of surface topology for biofilm development
13.4 Modification of surface to protect from biofilm-related biofouling
13.5 Sensors to monitor biofilm growth
13.6 Future scope
References
Chapter 14 Multi-species biofilms: Friends or foe to the human?
14.1 Introduction
14.2 Why focusing on multi-species biofilms?
14.3 Formation of multi-species biofilms and structural development
14.4 Detection methods of multi-species biofilms
14.5 Paradox of quorum sensing mechanisms and signaling in multi-species biofilms
14.6 Multi-species biofilms as foe of human
14.7 Multi-species biofilms as friends for human
14.8 Conclusion and future direction
Abbreviation used
References
Index