3D Lung Models for Regenerating Lung Tissue
- 1st Edition - August 10, 2022
- Imprint: Academic Press
- Editors: Gunilla Westergren-Thorsson, Sara Rolandsson Enes
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 9 0 8 7 1 - 9
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 9 0 8 7 2 - 6
3D Lung Models for Regenerating Lung Tissue is a comprehensive summary on the current state of art 3D lung models and novel techniques that can be used to regenerate lung tissue. W… Read more
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Request a sales quote3D Lung Models for Regenerating Lung Tissue is a comprehensive summary on the current state of art 3D lung models and novel techniques that can be used to regenerate lung tissue. Written by experts in the field, readers can expect to learn more about 3D lung models, novel techniques including bioprinting and advanced imaging techniques, as well as important knowledge about the complexity of the lung and its extracellular matrix composition.
Structured into 15 different chapters, the book spans from the original 2D cell culture model on plastic, to advanced 3D lung models such as using human extracellular matrix protein. In addition, the last chapters cover new techniques including 3D printing, bioprinting, and artificial intelligence that can be used to drive the field forward and some future perspectives. This highly topical book with chapters on everything from the complexity of the lung and its microenvironment to cutting-edge 3D lung models, represents an exciting body of work that can be used by researchers during study design, grant writing, as teaching material, or to stay updated with the progression of the field.
- A comprehensive summary of advanced 3D lung models written by the experts in the respiratory field
- Explore novel techniques that can be used to evaluate and improve 3D lung models, including techniques such as 3D printing, bioprinting, and artificial intelligence
- Explains what extracellular matrix is, the complexity of the lung microenvironment, and why this knowledge is important for creating a functional bioartificial lung
Senior researchers, clinicians, post-doctoral research fellows, PhD-students, medical residents, and master students with interest in the field of lung diseases and regenerative medicine
- Cover image
- Title page
- Table of Contents
- Copyright
- List of contributors
- Preface
- Chapter 1. Is the lung a complex organ to rebuild?
- Abstract
- Overview: the form, function, and beauty of the lung
- The mucus barrier and lung sentinel immunity
- The trachea and upper airways
- The airway epithelium
- Spatial organization of integrated innate and adaptive immunity in the lung
- The lung circulation
- Gas exchange: the core job of the lungs
- Bioengineering in the lungs is key to function
- The lung lymphatics
- Testing lung function
- The lung microbiome in normal lung homeostasis
- References
- Part I: 2D culture and the microenvironment
- Chapter 2. Two-dimensional cell culturing on glass and plastic: the past, the present, and the future
- Abstract
- Definition of two-dimensional cell culturing
- Glass: the two-dimensional surface in the beginning of cell culturing
- Glass: a two-dimensional surface used today
- Plastic for two-dimensional cell culturing
- Hydrophilicity, surface chemistry, and cell attachment
- Cells in two dimensions and three dimensions in the body and relevance for two-dimensional cell culturing
- The impact of switching between different kinds of surfaces and the role of medium composition
- Conclusions
- References
- Chapter 3. The importance of lung microenvironment
- Abstract
- Introduction
- Collagen and fibril formation
- Supportive glycoproteins
- Proteoglycans
- Glycosaminoglycan structure and function
- Microenvironments
- Conclusions
- References
- Part II: 3D lung models
- Chapter 4. The air–liquid interface model
- Abstract
- Introduction
- Air–liquid interface models of the lung epithelium: what can we measure?
- Single-cell RNA sequencing analysis of air–liquid interface–differentiated cell subtypes and states: how well do these represent the airway epithelium in vivo?
- Recapitulation of epithelial phenotype in air–liquid interface cultures from asthma patients
- Recapitulation of epithelial phenotype in air–liquid interface cultures from chronic obstructive pulmonary disease patients
- Use of air–liquid interface culture to study effects of environmental insults: respiratory virus
- Use of air–liquid interface culture to study effects of environmental insults: cigarette smoke
- Use of air–liquid interface culture to study effects of environmental insults: air pollution
- Concluding remarks
- References
- Chapter 5. Lung organoid models
- Abstract
- Introduction
- Mouse lung organoids
- Human adult lung organoids
- Human lung organoids—embryonic
- iPSC-derived lung organoids
- Disease models using lung organoids: chronic lung diseases
- Disease models using lung organoids: genetic lung diseases
- Disease models using lung organoids: viral lung infections
- Conclusion
- Acknowledgments
- References
- Chapter 6. Biomaterials for in vitro models in lung research
- Abstract
- Introduction to biomaterials
- Biomaterials for lung-related applications
- Conclusions
- Acknowledgment
- References
- Chapter 7. Three dimensional lung models - Three dimensional extracellular matrix models
- Abstract
- Introduction
- Extracellular matrix changes in chronic lung diseases
- Two-dimensional versus three-dimensional cell culture systems
- Three-dimensional models—1: single-protein models
- Three-dimensional models—2: extracellular matrix models with complex extracellular matrix mixtures
- Challenges
- Conclusions
- References
- Chapter 8. Lung-on-chip
- Abstract
- Introduction
- Brief historical background of organ-on chip technology
- Alveolus lung-on-chip models
- Mimicking lung parenchymal diseases on-chip
- Airway lung-on-chip models
- Application of lung-on-chip disease models with a focus on regenerative medicine
- Use of lung-on-chip models to study effects of environmental insults
- Advantages and challenges of lung-on-chip technology
- Concluding remarks
- References
- Chapter 9. Mechanical stimuli in lung regeneration
- Abstract
- Introduction
- Stiffness of the cell microenvironment
- Cell stretch
- Cell shear stress
- Microbioreactors for mechanically preconditioning lung cells
- Mechanical stimuli in whole lung bioreactors
- Conclusion
- Funding
- References
- Part III: New directions
- Chapter 10. Advanced manufacturing: three-dimensional printing and bioprinting of models of lung and airways
- Abstract
- Introduction
- Design considerations for manufactured lung scaffolds
- Biomaterials for manufacturing lung scaffolds
- Manufacturing porous lung scaffolds
- Manufacturing methods with true architecture control
- Four-dimensional printing and responsive materials
- Summary and future directions
- Acknowledgments
- References
- Chapter 11. Drug screening and high throughput in three-dimensional lung models
- Abstract
- Introduction
- The main difficulties associated with the identification of new pharmacological approaches
- How three-dimensional models can help to overcome these problems
- Concluding statements
- References
- Chapter 12. Model visualization: from micro to macro
- Abstract
- Model validation: from micro to macro
- Light versus electron microscopy
- Light microscopy across scales
- Conclusion
- Acknowledgments
- References
- Chapter 13. Artificial intelligence and computational modeling
- Abstract
- Introduction
- Definition and history of artificial intelligence and machine learning
- What is machine learning?
- What is deep learning?
- Machine learning in thoracic imaging
- Applications of machine learning in pulmonary diseases
- Challenges to further implementation of computer-aided diagnosis and radiomics
- Conclusion
- References
- Part IV: Concluding remarks and future directions
- Chapter 14. Challenges and opportunities for regenerating lung tissue using three-dimensional lung models
- Abstract
- Acknowledgments
- Index
- Edition: 1
- Published: August 10, 2022
- Imprint: Academic Press
- No. of pages: 260
- Language: English
- Paperback ISBN: 9780323908719
- eBook ISBN: 9780323908726
GW
Gunilla Westergren-Thorsson
Professor in lung biology with a specific focus on extracellular matrix linked to structural changes and lung function in human chronic lung diseases. During the past decade, her research focus has shifted more towards developing human 3D lung model systems and bioreactors. She was the Dean of the Medical Department at Lund University for 6 years, and one of the chairs of the national board of SciLifeLab, working with infrastructure in Life Science at a national level to facilitate the use and edge of different techniques.
Affiliations and expertise
Professor, Lund University, Lund, SwedenSE
Sara Rolandsson Enes
Dr. Enes is an associated researcher in lung biology with a specific focus on stem cells and their interactions with the microenvironment. During the recent years, her research focus has been on how the microenvironment affects the biology and function of stem cells and how this knowledge could be applied to improve cell therapy. In 2019, she was involved in putting together and leading a session on the topic “From 2D to 3D models of human lung disease: novel developments to rebuild the lung” for the large European Respiratory Society (ERS) annual congress. In addition, she was invited to write a landmark paper on bioartificial lungs.
Affiliations and expertise
Associated Researcher, Lund University, Lund, SwedenRead 3D Lung Models for Regenerating Lung Tissue on ScienceDirect