The Vitamins

Contributors to Volume VII

Preface

Contents of Other Volumes

Chapter 1. Principles of Microbiological Assay

I. Introduction

II. General Considerations

III. A Typical Assay Procedure

Chapter 2. Ascorbic Acid

Introduction

I. 2,6-Dichlorophenolindophenol Procedures

II. 2,4-Dinitrophenylhydrazine Procedures

III. Animal Assay

Chapter 3. Thiamine

Introduction

I. Extraction of Thiamine

II. Methods of Assay

III. Animal Assays for Thiamine

Chapter 4. Riboflavin

I. Introduction

II. Extraction of Riboflavin

III. Methods of Assay

Chapter 5. Niacin

I. Introduction

II. Microbiological Assay for Niacin, Niacinamide, and Closely Related Compounds

III. Chemical Methods for Determination of Niacin

IV. Determination of Niacinamide in Pharmaceutical Preparations

V. Determination of Methyl Derivatives of Niacin in Urine

VI. Determination of Pyridine Nucelotides

VII. Chromatography of Niacin Derivatives

VIII. Animal Assays for Niacin

Chapter 6. Vitamin B6

I. Microbiological Methods

II. Chromatographic Separation and Measurement of the Components of Vitamin B6

III. Determination of 4-Pyridoxic Acid

IV. Pyridoxal-5-Phosphate Measurements

V. Tryptophan Load Test and Measurement of Xanthurenic Acid in Urine as an Index of Vitamin B6 Deficiency

VI. Measurement of Blood Cell Transaminase Activity

VII. Animal Assays for Vitamin B6

Chapter 7. Pantothenic Acid

I. Introduction

II. Chemical Assay for Pantothenic Acid and Panthenol in Pharmaceutical Products

III. Microbiological Methods

IV. Chick Curative Bioassay for Total Pantothenic Acid

Chapter 8. Folic Acid

I. Introduction

II. Assay of Pharmaceutical Preparations by Official U.S. Pharmacopeia Methods

III. Estimations of Folate Activity in Natural Materials

IV. A Microbiological Assay for Folate Activity

Chapter 9. Vitamin B12

I. Introduction

II. Microbiological Methods

III. Radioactive Methods

IV. Conclusion

V. Protozoan Assays

Chapter 10. Biotin

I. Introduction

II. Microbiological Assays

III. Animal Assays

Chapter 11. Clinical Evaluation of Malnutrition

I. Introduction

II. Clinical Malnutrition—Its Development and Evaluation

III. Fundamentals of Nutritional Evaluation

IV. The Physical Examination

V. Summary

Author Index

Subject Index