Segmented Negative Strand Viruses: Arenaviruses, Bunyaviruses, and Orthomyxoviruses is a collection of proceedings presented at the 5th International Symposium on Negative Strand Viruses, held at Hilton Head, South Carolina, on September 11-17, 1983. The papers focus on three virus families with segmented negative RNA genomes: arenaviruses, bunyaviruses, and orthomyxoviruses. Emphasis is placed on the viruses' replication strategies, gene organization, and biochemical and biological properties. Comprised of 56 chapters, this volume begins with an introduction to the genome structure, transcription, and genetics of arenaviruses, bunyaviruses, and orthomyxoviruses. The next section is devoted to these viruses' gene expression, protein synthesis, and protein modification. Eukaryotic expression of cloned complementary DNA coding for influenza is considered, along with the functional expression and mutational analysis of influenza virus surface glycoproteins. Subsequent chapters explore viral proteins via antigenic and functional analyses; the biology and immunochemistry of the African arenaviruses Lassa and Mopeia; and the use of avian-human reassortant influenza A viruses as live vaccine viruses in humans. This book will be of interest to investigators, microbiologists, and molecular and cell biologists in fields such as molecular biology, genetics, viral immunology, infectious diseases, and vaccine development.