New Trends in Genetic Risk Assessment

Contributors

Participants

Preface

Acknowledgments

Introduction

1 The Role of Genotoxicity in Drug Safety Evaluation

Part I - Molecular Analysis of Mutagenesis

Introduction

2 DNA Repair and Genetic Alterations

3 Molecular Approaches to the Detection of Germinal Mutations in Mammalian Organisms, Including Man

4 Molecular analysis of Genetic and Epigenetic Alterations in Human Cells

5 Artificial Intelligence in the Study of the Structural Relationships Amongst Mutagens and Carcinogens

Part II - Detection of DNA Damage

Genotoxicity and Covalent Binding. An Introduction

6 Fluorimetric Analysis of DNA-strand Breakage and Repair Kinetics. Application to Radiotoxicology

7 Detection by 32P-postlabeling of Carcinogen-DNA Adducts in Animals and Man

8 SOS Chromotest and the Use of Bacteria for the Detection and Diagnosis of Genotoxic Agents

Part III - Gene Mutation

Introduction

9 Mutation at the hprt Locus in Human T-lymphocytes: In Vivo and In Vitro Studies

10 Mutagenicity and Clastogenicity Testing with Mouse Lymphoma Cells

11 Measurement of Mutant Frequency to 6-Thioguanine Resistance in Circulating T-lymphocytes for Human Population Monitoring

Part IV - Clastogenesis and Aneuploidy

Introduction

12 Specific Sites of Chromosomal Radiationinduced Rearrangements

13 Flow Cytometric Analysis and Sorting of Chromosomes

14 Flow Cytometric Detection of Micronuclei

15 Induced Chromosome Aneuploidy: its Role in the Assessment of the Genetic Toxicology of Environmental Chemicals

16 Assessment of Chemicallyinduced Aneuploidy: an Assay System Using a Mouse/Human Hybrid Cell Line

Part V - Germ Cells

Introduction

17 Prospective Overview on Germ Cell Mutations in Mice

18 Flow Cytometric Analysis of Toxic Chemicalinduced Alterations in Testicular Cell Kinetics and Sperm Chromatin Structure

Part VI - Test Selection and Risk Assessment

Introduction

19 Reflections on the Declining Ability of the Salmonella Assay to Detect Rodent Carcinogens as Positive

20 Methods for Genetic Toxicology Testing Strategies

21 New Approaches to Battery Selection and Interpretation

22 Risk Assessment of Genotoxic Agents: Problems and Possible Solutions

23 Genetic Risk Estimation: Experience with Ionizing Radiation and its Relevance for Mutagenic Chemicals

Color Plates Section

Index