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Methods in Cell Physiology
Volume I
- 1st Edition - January 1, 1964
- Editor: David M. Prescott
- Language: English
- Paperback ISBN:9 7 8 - 1 - 4 8 3 2 - 5 5 4 3 - 9
- Hardback ISBN:9 7 8 - 1 - 4 8 3 2 - 3 2 1 8 - 8
- eBook ISBN:9 7 8 - 1 - 4 8 3 2 - 6 4 0 3 - 5
Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which… Read more
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Request a sales quoteMethods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included. The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.
List of Contributors
Preface
1. Survey of Cytochemistry
I. Survey
II. Summary and Conclusions
References
2. Methods of Culture for Plasmodial Myxomycetes
I. Introduction
II. Culture of Impure Myxomycètes
III. Isolation and Pure Culture
IV. Axenic Culture of Physarum polycephalum on Soluble Media
V. Sporulation (Fruiting) of Physarum polycephalum in Pure Culture
VI. Sclerotia and Spherule Formation
VII. Changes Observed after Prolonged Growth in Pure Culture
References
3. Mitotic Synchrony in the Plasmodia of Physarum Polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia
I. Introduction
II. Life History of Physarum Polycephalum
III. The Experimental Material
IV. Discussion
V. Summary
References
4. Introduction of Synchronous Encystment (Differentiation) in Acanthamoeba sp.
I. Introduction
II. The Encystment Process: A Description
III. Induction of Synchronous Encystment: The Method
IV. Optimal Conditions for Synchronous Encystment
V. Evaluation of the Method
References
5. Experimental Procedures and Cultural Methods for Euplotes Eurystomus and Amoeba Proteus
I. Introduction
II. Euplotes Eurystomus
III. Amoeba Proteus
References
6. Nuclear Transplantation in Ameba
I. Introduction
II. Equipment
III. Operating Procedure
IV. Conclusion
References
7. Experimental Techniques with Ciliates
I. Introduction
II. Experimental Possibilities with Stentor
III. Techniques with Paramecium
References
8. Methods for Using Tetrahymena in Studies of the Normal Cell Cycle
I. Introduction
II. Special Equipment
III. Manipulation of Cells
References
9. Continuous Synchronous Cultures of Protozoa
I. Introduction
II. Light-Induced Synchrony in Euglena Gracilis
III. Temperature-Induced Synchrony in Astasia Longa
IV. Concluding Remarks
References
10. Handling and Culturing of Chlorella
I. Introduction
II. The Organisms and Their Growth
III. The Culture of Chlorella
Appendix
References
11. Culturing and Experimental Manipulation of Acetabularia
I. Introduction to the Organism
II. Culture Techniques
III. Microsurgery
IV. Biochemical Analysis
V. Potentialities and Limitations
References
12. Handling of Root Tips
I. Introduction
II. Germination of Seeds and Growing of Roots
III. Fixation of Root Tips
IV. Staining of Cells
V. Making Slides Permanent
VI. Autoradiographic Methods
VII. Isolation of Nuclei
VIII. Special Uses
IX.Conclusion
References
13. Grasshopper Neuroblast Techniques
I. Introduction
II. Selection of Species
III. Collecting
IV. Rearing
V. Eggs
VI. Development
VII. Culture Techniques
VIII. Separated Cell Techniques
IX. Fixed Cell Techniques
X. Radiation Techniques
XI. Chemical Techniques
XII. Gas Techniques
XIII. Microdissection
References
14. Measurement of Material Uptake by Cells: Pinocytosis
I. Introduction
II. Demonstration of Pinocytosis
III. Inducers of Pinocytosis
IV. Quantitative Estimation of Pinocytic Uptake
V. Significance of the Values Obtained and Errors Likely to Be Encountered in the Different Methods
References
15. Quantitative Autoradiography
I. Introduction
II. Theoretical Considerations
III. Practical Considerations and Techniques
IV. The Problem of "Intermediates"
References
16. High-Resolution Autoradiography
I. Introduction
II. Methods
III. Discussion of the Methods
IV. Resolution
V. Discussion
References
17. Autoradiography with Liquid Emulsion
I. Introduction
II. Emulsions
III. Procedure
References
18. Autoradiography of Water-Soluble Materials
I. Introduction
II. Procedures
III. Experimental Example
References
19. Preparation of Mammalian Metaphase Chromosomes for Autoradiography
I. Introduction
II. Procedure
III. Examples and Variation of Technique
References
20. Methods for Measuring the Length of the Mitotic Cycle and the Timing of DNA Synthesis for Mammalian Cells in Culture
I. Introduction
II. Measurements of Intermitotic Times by Time-Lapse
III. Cinemicrography
IV. Measurements of Growth Rates of Cultures
V. Measurement of Parts of Interphase
VI. Summary
References
21. Micrurgy of Tissue Culture Cells
I. Introduction
II. Equipment
III. Handling of Cells
IV. Conclusion
References
22. Microextraction and Microelectrophoresis for Determination and Analysis of Nucleic Acids in Isolated Cellular Units
I. Introduction
II. Methods
III. Accuracy
IV. Applications
V. Concluding Remarks
VI. Appendices
References
Author Index
Subject Index
- No. of pages: 480
- Language: English
- Edition: 1
- Published: January 1, 1964
- Imprint: Academic Press
- Paperback ISBN: 9781483255439
- Hardback ISBN: 9781483232188
- eBook ISBN: 9781483264035
DP
David M. Prescott
Affiliations and expertise
BIOLOGY DIVISION, OAK RIDGE NATIONAL LABORATORY
OAK RIDGE, TENNESSEE