Methods in Cell Physiology

Volume I

1st Edition - January 1, 1964
Editor: David M. Prescott
Language: English
Paperback ISBN:
9 7 8 - 1 - 4 8 3 2 - 5 5 4 3 - 9
Hardback ISBN:
9 7 8 - 1 - 4 8 3 2 - 3 2 1 8 - 8
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 6 4 0 3 - 5

Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which… Read more

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Methods in Cell Physiology, Volume I, brings together into one compilation, complete and detailed treatments of a number of widely useful techniques in modern cell biology which have not been published in full form elsewhere in the literature. The presentations in this volume are comprehensive to the extent that they may serve not only as a practical introduction to experimental procedures but they also provide some evaluation of the limitations, potentialities, and current applications of the methods. Only those theoretical considerations needed for proper use of the method are included. The book may have particular usefulness for those working with intact cells, and the first chapters deal with culturing and experimental manipulation of a variety of cell types. There are numerous descriptions of techniques designed for working with single cells and of procedures for studying cellular activities in relation to the cell life cycle. The chapters on autoradiography include detailed treatment of qualitative and quantitative aspects of the method as well as simple procedures for using liquid and dry emulsions.

List of Contributors

Preface

1. Survey of Cytochemistry

I. Survey

II. Summary and Conclusions

References

2. Methods of Culture for Plasmodial Myxomycetes

I. Introduction

II. Culture of Impure Myxomycètes

III. Isolation and Pure Culture

IV. Axenic Culture of Physarum polycephalum on Soluble Media

V. Sporulation (Fruiting) of Physarum polycephalum in Pure Culture

VI. Sclerotia and Spherule Formation

VII. Changes Observed after Prolonged Growth in Pure Culture

References

3. Mitotic Synchrony in the Plasmodia of Physarum Polycephalum and Mitotic Synchronization by Coalescence of Microplasmodia

I. Introduction

II. Life History of Physarum Polycephalum

III. The Experimental Material

IV. Discussion

V. Summary

References

4. Introduction of Synchronous Encystment (Differentiation) in Acanthamoeba sp.

I. Introduction

II. The Encystment Process: A Description

III. Induction of Synchronous Encystment: The Method

IV. Optimal Conditions for Synchronous Encystment

V. Evaluation of the Method

References

5. Experimental Procedures and Cultural Methods for Euplotes Eurystomus and Amoeba Proteus

I. Introduction

II. Euplotes Eurystomus

III. Amoeba Proteus

References

6. Nuclear Transplantation in Ameba

I. Introduction

II. Equipment

III. Operating Procedure

IV. Conclusion

References

7. Experimental Techniques with Ciliates

I. Introduction

II. Experimental Possibilities with Stentor

III. Techniques with Paramecium

References

8. Methods for Using Tetrahymena in Studies of the Normal Cell Cycle

I. Introduction

II. Special Equipment

III. Manipulation of Cells

References

9. Continuous Synchronous Cultures of Protozoa

I. Introduction

II. Light-Induced Synchrony in Euglena Gracilis

III. Temperature-Induced Synchrony in Astasia Longa

IV. Concluding Remarks

References

10. Handling and Culturing of Chlorella

I. Introduction

II. The Organisms and Their Growth

III. The Culture of Chlorella

Appendix

References

11. Culturing and Experimental Manipulation of Acetabularia

I. Introduction to the Organism

II. Culture Techniques

III. Microsurgery

IV. Biochemical Analysis

V. Potentialities and Limitations

References

12. Handling of Root Tips

I. Introduction

II. Germination of Seeds and Growing of Roots

III. Fixation of Root Tips

IV. Staining of Cells

V. Making Slides Permanent

VI. Autoradiographic Methods

VII. Isolation of Nuclei

VIII. Special Uses

IX.Conclusion

References

13. Grasshopper Neuroblast Techniques

I. Introduction

II. Selection of Species

III. Collecting

IV. Rearing

V. Eggs

VI. Development

VII. Culture Techniques

VIII. Separated Cell Techniques

IX. Fixed Cell Techniques

X. Radiation Techniques

XI. Chemical Techniques

XII. Gas Techniques

XIII. Microdissection

References

14. Measurement of Material Uptake by Cells: Pinocytosis

I. Introduction

II. Demonstration of Pinocytosis

III. Inducers of Pinocytosis

IV. Quantitative Estimation of Pinocytic Uptake

V. Significance of the Values Obtained and Errors Likely to Be Encountered in the Different Methods

References

15. Quantitative Autoradiography

I. Introduction

II. Theoretical Considerations

III. Practical Considerations and Techniques

IV. The Problem of "Intermediates"

References

16. High-Resolution Autoradiography

I. Introduction

II. Methods

III. Discussion of the Methods

IV. Resolution

V. Discussion

References

17. Autoradiography with Liquid Emulsion

I. Introduction

II. Emulsions

III. Procedure

References

18. Autoradiography of Water-Soluble Materials

I. Introduction

II. Procedures

III. Experimental Example

References

19. Preparation of Mammalian Metaphase Chromosomes for Autoradiography

I. Introduction

II. Procedure

III. Examples and Variation of Technique

References

20. Methods for Measuring the Length of the Mitotic Cycle and the Timing of DNA Synthesis for Mammalian Cells in Culture

I. Introduction

II. Measurements of Intermitotic Times by Time-Lapse

III. Cinemicrography

IV. Measurements of Growth Rates of Cultures

V. Measurement of Parts of Interphase

VI. Summary

References

21. Micrurgy of Tissue Culture Cells

I. Introduction

II. Equipment

III. Handling of Cells

IV. Conclusion

References

22. Microextraction and Microelectrophoresis for Determination and Analysis of Nucleic Acids in Isolated Cellular Units

I. Introduction

II. Methods

III. Accuracy

IV. Applications

V. Concluding Remarks

VI. Appendices

References

Author Index

Subject Index

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Methods in Cell Physiology

Volume I

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David M. Prescott