Autoradiography in Biology and Medicine

ContentsPart I Theory I. Introduction Historical, What Is Autoradiography?, The Photographic Emulsion As A Scientific Instrument, The Biologist and Autoradiography, The Etymology of Autoradiography II. The Photographic Process Introduction, The Emulsion, The Latent Image, Development, Latent-Image Potential, Fixation, Washing, Drying III. Comparative Response of Commercial and Scientific Emulsions Introduction, Response of Films To Electrons, Response of Films To Beta Ray Spectra of Isotopes, Response of Films to X-Rays and Gamma Rays IV. Commercial Photographic Materials For Autoradiography X-Ray Films, Nuclear Emulsions, Stripping Films and Plates, Gels and Pellicles, X-Ray Emulsions on Plate V. The Autoradiographic Image Observing The Image, Track Images, Random Grain Images, Realignment Techniques, Publishing The Autoradiogram VI. Resolution Theoretical Considerations, Experimental Results on Variables Affecting Resolution VII. Estimating Dose and Exposure Time Type of Information Desired, Chemical Variables, Variables of The Biological Laboratory, Autoradiographic Technique, Doses Reported in The Literature, Estimating The Exposure Time VIII. Sources of Error Leaching The Radioactivity During the Histologic Preparation, Errors Occurring During the Preparation of The Autoradiogram for Exposure, Errors Occurring During Exposure—Chemical Effects, Errors Occurring During Exposure—Other Miscellaneous, Errors Occurring During Photographic and Histologic Processing, Other ErrorsPart II Techniques Introduction IX. Nonflat Surfaces Contour Autoradiography, Autoradiography Plus Roentgenography of Patients X. Flat Surfaces—Macrosections and Plants Freezing and Slicing Soft Tissues, Bones Embedded in Plaster of Paris, Bones Mounted on Boards, Freezing and Sawing Soft and Hard Tissues Combined, Plants XI. Flat Surfaces—Microsections Apposition Methods for Microscopic Sections and Smears, Wet Mounting of Tissue Sections, Dry Mounting of Tissue Sections, Coating With Liquid Emulsion—Zoological Materials, Coating With Liquid Emulsion—Plant Materials, Coating With Permeable-Base Stripping Film, Coating and Inverting, Impermeable-Base Stripping Film Technique, The Wet Process, Constant Registry Autoradiography XII. Cells, Dusts, and Other Microscopic Particulate Matter Impermeable-Base Stripping Film Technique, Smearing Suspensions of Cells on A Photographic Plate, Staining Bone-Marrow Cells Through Permeable-Base Stripping Film, Air-Borne and Other Particular Matter XIII. Fluids XIV. Techniques for Preparing Histologic Sections of Undecalcified Bone and Teeth Embedding in Bio-Plastic, Embedding in M4700 With Plasticizer, Embedding in M4700 without Plasticizer, Rapid Embedding in R-250 Astrolite, Rapid Embedding in Methyl Methacrylate, Microsawing of Histologic Sections XV. Miscellaneous Thin Protective Films, Transferring Frozen Histologic Sections to Plates and Slides, Probe Autoradiography, Autoradiography by Photoelectron Emission Microscopy, Pinhole AutoradiographyPart III Bibliography 1. Introduction 2. Bibliography 3. Author Index to the Bibliography 4. Classified Subject Index to the Bibliography 5. Supplement to the Bibliography 6. Books for Supplemental Reading Index