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Planar Gel Electrophoresis
For Protein Separation
- 1st Edition - August 1, 2029
- Editor: Teck Yew Low
- Language: English
- Paperback ISBN:9 7 8 - 0 - 3 2 3 - 8 5 4 7 3 - 3
- eBook ISBN:9 7 8 - 0 - 3 2 3 - 8 5 4 7 4 - 0
Planar Gel Electrophoresis: For Protein Separation presents the state-of-the-art in planar gel electrophoretic techniques and how they can be implemented in laboratory practi… Read more
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Request a sales quotePlanar Gel Electrophoresis: For Protein Separation presents the state-of-the-art in planar gel electrophoretic techniques and how they can be implemented in laboratory practice for protein separation. The book covers the whole of the subject, including its historical background, concepts, applications and new developments in planar gel electrophoresis in protein separation. Chapters are written by experts with deep experience in their field. This volume serves as a practical guide for researchers to choose and utilize suitable sample preparation procedures, also describing notable contemporary uses in planar gel electrophoresis in industrial and academic project environments.
- Contains the latest developments and applications of planar gel electrophoresis for protein separation
- Guides scientists in choosing the appropriate techniques to use to solve their problems, with an emphasis on the biological functions of the studies
- Provides an overview of each topic covered, with a focus on the big picture as well as the most relevant information for project planning
Libraries, academics, and laboratory managers in industry and research centers
Professional scientists working in analytical chemistry labs, pharma or biotech companies
Professional scientists working in analytical chemistry labs, pharma or biotech companies
Section 1: History, Theory and Milestones
1. Historical backgrounds and milestones
2. Theory and core concepts
3. Different gel chemistry and buffer systems
Section 2: Sample preparation
4. Preparation of protein samples
Section 3: Different electrophoretic techniques
5. SDS-PAGE (Denaturing gels)
6. Non-denaturing gels
7. Non-reducing gels
8. Gradient gels to cover high MW and low MW proteins
9. Separation of low MW peptides with Tris-Tricine gels
10. Agarose Gels for Separating High MW Proteins
11. Preparative gels
12. Acid- urea – triton gel for separating baisc proteins and histones
13. Blue native gels for separating protein complex
14. Pore gradient gel electrophoresis (PGGE)
15. Cationic Electrophoresis
16. Isoelectric focusing (IEF)
17. Immobilized pH gradients (IPG)
18. Two-dimenstional gel electrophoresis (2-DE)
19. Difference gel electrophoresis (DIGE)
20. Diagonal Electrophoresis for disulfide bond detection
21. Affinity gel electrophoresis (AEP)
22. Electrophoretic mobility shift assays (EMSA)
23. Immobilized metal affinity electrophoresis (IMAEP)
24. 1D and 2D zymography (1DZ and 2DZ)
25. SARCOSYL-PAGE: separation of PEGylated proteins
26. Phenylboronate acrylamide gel electrophoresis for glycated proteins
27. Field Inversion gel electrophoresis (FIGE)
28. Microfluidic gel electrophoresis
Section 4: Post-electrophoresis analysis
29. Visualization of gel separated proteins with protein stains
30. Densitometry analysis of protein bands or spots
31. Data analysis software for extracting information from gel separations
32. Recovery of proteins from gel with electroelution
33. In-gel proteolytic digestion of proteins and mass spectrometry
Section 5: Coulping electrophoresis with antibody detection
34. Western Blotting
35. Serological Proteome Analysis (SERPA)
36. Immunofixation Electrophoresis (IFE)
37. Immunoelectrophoresis (IEP)
1. Historical backgrounds and milestones
2. Theory and core concepts
3. Different gel chemistry and buffer systems
Section 2: Sample preparation
4. Preparation of protein samples
Section 3: Different electrophoretic techniques
5. SDS-PAGE (Denaturing gels)
6. Non-denaturing gels
7. Non-reducing gels
8. Gradient gels to cover high MW and low MW proteins
9. Separation of low MW peptides with Tris-Tricine gels
10. Agarose Gels for Separating High MW Proteins
11. Preparative gels
12. Acid- urea – triton gel for separating baisc proteins and histones
13. Blue native gels for separating protein complex
14. Pore gradient gel electrophoresis (PGGE)
15. Cationic Electrophoresis
16. Isoelectric focusing (IEF)
17. Immobilized pH gradients (IPG)
18. Two-dimenstional gel electrophoresis (2-DE)
19. Difference gel electrophoresis (DIGE)
20. Diagonal Electrophoresis for disulfide bond detection
21. Affinity gel electrophoresis (AEP)
22. Electrophoretic mobility shift assays (EMSA)
23. Immobilized metal affinity electrophoresis (IMAEP)
24. 1D and 2D zymography (1DZ and 2DZ)
25. SARCOSYL-PAGE: separation of PEGylated proteins
26. Phenylboronate acrylamide gel electrophoresis for glycated proteins
27. Field Inversion gel electrophoresis (FIGE)
28. Microfluidic gel electrophoresis
Section 4: Post-electrophoresis analysis
29. Visualization of gel separated proteins with protein stains
30. Densitometry analysis of protein bands or spots
31. Data analysis software for extracting information from gel separations
32. Recovery of proteins from gel with electroelution
33. In-gel proteolytic digestion of proteins and mass spectrometry
Section 5: Coulping electrophoresis with antibody detection
34. Western Blotting
35. Serological Proteome Analysis (SERPA)
36. Immunofixation Electrophoresis (IFE)
37. Immunoelectrophoresis (IEP)
- No. of pages: 586
- Language: English
- Edition: 1
- Published: August 1, 2029
- Imprint: Elsevier
- Paperback ISBN: 9780323854733
- eBook ISBN: 9780323854740
TL
Teck Yew Low
Dr Teck Yew Low is currently an Associate Professor and Senior Research Fellow at the UKM Medical Molecular Biology Institute (UMBI), National University of Malaysia (UKM), where he leads the cancer proteomics and metabolomics group. He obtained his PhD in Biochemistry from the National University of Singapore (NUS) and worked with mass spectrometry-based proteomics in the Genome Institute of Singapore (GIS), the Center for Experimental Bioinformatic (CEBI) in University of Southern Denmark (SDU) and the Netherlands Proteomics Center (NPC) in Utrecht University in the Netherlands. His research interests include bridging proteomics with clinics, phosphoproteomics and cancer proteogenomics in the Malaysian population.
Affiliations and expertise
Associate Professor, UKM Medical Molecular biology Institute (UMBI), Kuala Lumpur, Malaysia