Limited Offer
Save 50% on book bundles
Immediately download your ebook while waiting for your print delivery. No promo code needed.
Biophysical Mass Spectrometry
Protein Higher Order Structure Elucidation and Integration with Biophysical Techniques
- 1st Edition - September 1, 2099
- Editors: Darryl L. Davis, Frank Sobott
- Language: English
- Hardback ISBN:9 7 8 - 0 - 1 2 - 8 2 2 5 1 0 - 3
- eBook ISBN:9 7 8 - 0 - 1 2 - 8 2 2 5 1 1 - 0
The structural characterization of proteins is typically performed in labs that have spectroscopy-based biophysical tools; in moving to techniques that can provide more… Read more
Purchase options
Institutional subscription on ScienceDirect
Request a sales quoteThe structural characterization of proteins is typically performed in labs that have spectroscopy-based biophysical tools; in moving to techniques that can provide more granularity, a different set of skills is needed. Biophysical Mass Spectrometry provides a current and comprehensive assessment of the use of mass spectrometry in higher order structure characterization of proteins, with an emphasis on monoclonal antibodies as the predominant platform for biopharma. It aims to demonstrate how new and established techniques can be integrated. The book provides a reference to those wishing to pursue the adoption of new methods in the laboratory-based structural characterization of proteins.
- Reviews current structural mass spectrometry techniques for use in the biopharmaceutical industry
- Highlights new methodologies and focusses on integrating structural techniques
- Gives in-depth reference information for those starting a lab devoted to the structural characterization of proteins
- Provides sufficient detail for bench scientists to develop and implement structural mass spectrometry methods
- Demonstrates how new and established techniques can be integrated
1. Do you agree with the author’s assessment of the proposed audience? (Please add your own suggestions regarding the target readership).
Yes, but more emphasis should be placed on academic researcher in Departments of Biochemistry, Biophysics, Chemistry
2. Based on your assessment of the content, in your view who is the audience for the project? Please include subject area, level of expertise, and method of usage.
Investigators in biotechnology but also in academics. Newcomers to field. PhD students and postdocs.
Yes, but more emphasis should be placed on academic researcher in Departments of Biochemistry, Biophysics, Chemistry
2. Based on your assessment of the content, in your view who is the audience for the project? Please include subject area, level of expertise, and method of usage.
Investigators in biotechnology but also in academics. Newcomers to field. PhD students and postdocs.
1. Protein Structure: Higher Order Structure
2. Protein Stability
3. Heterogeneity of Proteins: Structure Function as related to HOS
4. Conventional Biophysical Techniques and Emerging Technologies
5. Native MS-Intact Protein Mass Spectrometry
6. Ion Mobility
7. Cross-Linking
8. HDX
9. Covalent Labeling Techniiques for Higher Order Structure
10. Informatics
11. Separation Assays and HOS
12. Integrated HOS techniques
2. Protein Stability
3. Heterogeneity of Proteins: Structure Function as related to HOS
4. Conventional Biophysical Techniques and Emerging Technologies
5. Native MS-Intact Protein Mass Spectrometry
6. Ion Mobility
7. Cross-Linking
8. HDX
9. Covalent Labeling Techniiques for Higher Order Structure
10. Informatics
11. Separation Assays and HOS
12. Integrated HOS techniques
- No. of pages: 800
- Language: English
- Edition: 1
- Published: September 1, 2099
- Imprint: Academic Press
- Hardback ISBN: 9780128225103
- eBook ISBN: 9780128225110
DD
Darryl L. Davis
Dr Daryl L. Davis is Associate Director at Janssen Research and Development LLC. He has worked in bioanalytical labs within the biopharmaceutical industry since 2000, including on several HOS techniques using mass spectrometry such as HDX, cross-linking, covalent labelling, and various proteolysis methods. His graduate work focused on using mass spectrometry to characterise kinases and their phosphorylated targets. He has edited a series on monoclonal antibody (mAb) characterization that helped to establish the first national mAb reference material.
Affiliations and expertise
Associate Director, Janssen Research and Development LLC.FS
Frank Sobott
Professor Frank Sobott is Chair of Biomolecular Mass Spectrometry at the University of Leeds in the UK, and part of the Asbiry Centre for Structural Molecular Biology and the School of Molecular and Cellular Biology. He holds a PhD from Frankfurt and carried out postgraduate work at the University of Oxford. His group develops and applies new mass spectrometry methodologies to analyse the structure and functional behaviour of biomolecules and biomolecular complexes.
Affiliations and expertise
Professor and Chair of Biomolecular Mass Spectrometry, University of Leeds, UK; part of the Asbiry Centre for Structural Molecular Biology and the School of Molecular and Cellular Biology