Books in Molecular biology cellular biology and genetics

Novel Methods and Pathways in Cancer Glycobiology Research

1st Edition
Volume 157
February 2, 2023
Charles J Dimitroff + 1 more
English
Hardback
9 7 8 0 3 2 3 9 9 1 7 7 3
eBook
9 7 8 0 3 2 3 9 9 1 7 8 0

Cancer glycobiology is a blazing hot area of cancer research with aberrant post-translational glycosylations on lipids and proteins considered critical for cancer development and metastasis. The NIH/NCI Alliance of Glycobiologists for Cancer Research is a consortium of (10) tumor glycobiology laboratories funded to investigate how cancer-associated glycans (CAG) impact the development and metastasis of cancer. The hope of this momentous effort is to discover CAGs for use as novel biomarkers to detect early-stage cancer or its aggressive behavior or, alternatively, as novel molecular targets with the promise of a curative approach to cancer therapy. The mission of the ‘Alliance’ is to study structure and function of CAGs in cancer development and to provide clinically useful biomarkers for detecting cancer early or the potential of metastasis. This charge has stimulated extensive collaborations across US and International institutions to accelerate discovery of glycan-based biomarkers to the forefront of NCI's efforts to diagnose early-stage cancer and provide curative anti-cancer therapies. The main goals of these research efforts are: 1. To identify and study how cancer-associated glycans functionally impact cancer growth and metastasis2. To identify cancer-associated glycans that can serve as biomarkers for the detection or progression of cancer3. To develop cancer-associated glycans as novel therapeutic targets for the treatment and cure of cancer This volume consists of (9) chapters, one from each funded laboratory, that reviews new methods and mechanisms highlighting glycoconjugates and their roles as cancer biomarkers and/or anti-cancer therapeutic targets.

Radiation Oncology and Radiotherapy Part B

1st Edition
Volume 174
January 31, 2023
Ai Sato + 3 more
English
Hardback
9 7 8 0 3 2 3 8 9 9 4 7 5
eBook
9 7 8 0 3 2 3 8 9 9 4 8 2

Radiation Oncology and Radiotherapy, Part B, Volume 174 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics including CT-assisted focal irradiation of tumors in mice, Methods to preserve correct dosimetry in small animal irradiators, Monitoring TGFbeta signaling in irradiated tumors, Cytofluorometric characterization of the lymphoid compartment of irradiated tumors, Cytofluorometric characterization of the myeloid compartment of irradiated tumors, Mass cytometry to characterize the immune infiltrate of irradiated tumors, Characterization of the immune infiltrate in irradiated mouse tumor by multiplex immunofluorescence, and much more. Other chapters cover Methods to study FOXP3+CD8+ cells in irradiated bone metastases, Luminex-based characterization of cytokine signaling by irradiated tumors, Explant-based assessment of anticancer immunity in irradiated tumors, Pipeline to characterize the TCR repertoire of irradiated tumors, Pipeline to identify tumor neoantigens exposed by radiation, and more.

Transcription and Translation in Health and Disease

1st Edition
January 30, 2023
Manoj Garg + 2 more
English
Paperback
9 7 8 0 3 2 3 9 9 5 2 1 4
eBook
9 7 8 0 3 2 3 9 9 5 2 2 1

Transcription and Translation in Health and Disease provides a detailed overview of the regulators underlying transcription and translation in relation to a variety of human diseases and disorders. Beginning with an introduction into the current perspectives relating to these processes in human disease, the book expands to focus on specific mechanisms underlying conditions such as arthritis, cancer, neurological disorders, diabetes and cardiovascular disease. This book considers RNA processing and related mechanisms in eukaryotes including RNA splicing, RNA binding proteins, RNA interference, microRNAs, RNA editing, transcription factors, RNAi screening, CRISPR activation, CRISPR-Cas9 interference, and post-translational modifications. It provides a structured and detailed overview of the various regulators underlying molecular processes and their impact on health and disease, equipping readers with the necessary knowledge for further investigation in the areas of treatment and therapeutic intervention.

Principles of Genomics and Proteomics

1st Edition
January 25, 2023
Rakeeb Ahmad Mir + 2 more
English
Paperback
9 7 8 0 3 2 3 9 9 0 4 5 5
eBook
9 7 8 0 3 2 3 9 9 3 7 8 4

Principles of Genomics and Proteomics is the perfect reference for graduate students and researchers in these areas to understand its principles and execute precise and reproducible experiments. Following an introductory chapter, the book dives into proper research, including genome mapping. Experiments covered in the book span from Sangers Sequencing, Shotgun sequencing, SAGE analysis, DNA footprinting, Gel retardation, ChIP, and protein resolution methods, including PAGE, 2D gel electrophoresis and isoelectric focusing. Biophysical techniques are also described in detail, including ultraviolet and visible light spectroscopy, fluorescence spectroscopy, NMR and X-ray diffraction. A final proteome analysis is dedicated to functional analysis. Other chapters cover applications of omics technologies broadly. This book is the perfect reference for genetics labs around the world. Graduate students will benefit from the structured and detailed coverage of methods and established researchers will benefit from the book for staff training in research and may find it particularly helpful in enhancing reproducibility of experiments.

Integrated Methods in Protein Biochemistry: Part B

1st Edition
Volume 679
January 18, 2023
Arun K. Shukla
English
Hardback
9 7 8 0 3 2 3 9 9 2 6 4 0
eBook
9 7 8 0 3 2 3 9 9 2 6 5 7

Integrated Methods in Protein Biochemistry: Part B, Volume 678 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Precise modification of native proteins, purification, and analysis of bioconjugates, NanoBiT-based methods to monitor the activation and modulation of RTKs, The interplay of G-protein βγ subunit and PLC-β enzyme in PIP2 hydrolysis and downstream signaling, Structure and function of bacterial secretion system, Tools and protocols for probing protein sumoylation, Spectroscopic analysis of cysteine dioxygenase: a mammalian thiol-dioxygenase, DeGlyPHER: MS-based analysis of viral spike N-glycoforms, and more. Additional sections cover Covalent protein painting: MS-based protein footprinting, Characterization of GPCR signaling complexes using negative-staining electron microscopy, Probing protein misfolding and dissociation with free electron laser, Optimized protocol for the characterization of Cas12a activities, Proximity proteomics for the identification and characterization of extracellular vesicles, Structural and Functional characterization of lytic polysaccharide monooxygenases, and much more.

The Immunological Synapse Part A

1st Edition
Volume 173
January 11, 2023
Clément Thomas + 1 more
English
Hardback
9 7 8 0 3 2 3 9 0 1 5 4 3
eBook
9 7 8 0 3 2 3 9 0 1 5 5 0

The Immunological Synapse, Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. Sections cover Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators, Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse, P815-based redirected degranulation assay to study human NK cell effector functions, Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging, Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay, and more. Other chapters focus on the Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, An in vitro model to monitor natural killer cell effector functions against primary breast cancer, and Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar L.

Cell Biology

4th Edition
December 13, 2022
Thomas D. Pollard + 3 more
English

Reader-friendly Cell Biology, 4th Edition, provides a concise but comprehensive foundation for students entering research or health care career paths. Award winning illustrations help readers quickly grasp general principles. The authors have thoroughly updated this popular text to provide readers with the current understanding of the principles of normal cellular function along with examples of how molecular defects predispose to human disease. Major new themes in the 4th edition include the roles of intrinsically disordered polypeptides and phase separation in cellular functions, the influence of new molecular structures on understanding mechanisms, and the impact of exciting new methods—from single cell RNA sequencing to second generation super resolution fluorescence microscopy—on advancing our understanding.

Cell Biology

4th Edition
December 13, 2022
Thomas D. Pollard + 3 more
English

Cell Biology

4th Edition
December 13, 2022
Thomas D. Pollard + 3 more
English

Cell Biology

4th Edition
December 13, 2022
Thomas D. Pollard + 3 more
English

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health

Books in Molecular biology cellular biology and genetics

Novel Methods and Pathways in Cancer Glycobiology Research

Radiation Oncology and Radiotherapy Part B

Transcription and Translation in Health and Disease

Principles of Genomics and Proteomics

Integrated Methods in Protein Biochemistry: Part B

The Immunological Synapse Part A

Cell Biology

Cell Biology

Cell Biology

Cell Biology

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