Books in Biochemistry biophysics

RNA Enzymology and Technology

1st Edition
Volume 725
December 15, 2025
Kathy Liu
English

This volume of Methods in Enzymology brings together a comprehensive collection of experimental approaches and mechanistic insights into enzymes work on RNAs—molecular catalysts at the heart of RNA metabolism, regulation, and therapeutics. Enzymes work on RNAs are essential for a wide array of biological processes, from gene expression and RNA processing to translation control, RNA silencing, and genome integrity. The volume is organized into six thematic sections that reflect the multifaceted roles of RNA enzymes in biology and medicine. Together, these chapters—authored by leading experts in the field—provide an essential resource for researchers seeking to understand and manipulate RNA enzyme function using state-of-the-art biochemical, biophysical, and therapeutic approaches.

Transketolases

1st Edition
Volume 724
December 5, 2025
Franck Charmantray
English

Transketolase is an ubiquitous enzyme which has not yet finished revealing all its secrets. This Methods in Enzymology: Transketolases volume is devoted to the description of cutting-edge methods to its study in biochemistry. Several topics are addressed by world experts in their field.The first part of this volume deals with transketolase at the cellular level. The latest scientific progress on human transketolase TKL1 in relation to certain diseases and to its inhibition are discussed including the involvement of this enzyme in new metabolic pathways or as a diagnostic biomarker.The second part focuses on transketolase at the molecular level. The structural and mechanistic aspects linked to this enzyme are addressed using several approaches including molecular modelling and crystallography, for example. Finally, the experimental methodological approaches relating to the use of transketolase as a tool of choice in biocatalysis is presented.

Antimicrobial Peptides and Proteins

1st Edition
Volume 149
December 1, 2025
Rossen Donev
English
Hardback
9 7 8 0 4 4 3 3 4 5 1 9 7
eBook
9 7 8 0 4 4 3 3 4 5 2 0 3

Antimicrobial Peptides and Proteins, Volume 148 in the Advances in Protein Chemistry and Structural Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Nature and mechanistic action of Antimicrobial Peptides, Advances in elucidation molecular targets and mechanisms of action of AMPs: beyond membrane disruption, Discovery, Design, and Applications of Antimicrobial Peptides, Designing Potent Antimicrobial Peptides Against Acinetobacter baumannii: A Combined Approach Using Genetic Algorithms and Machine Learning Strategies, Protection of Diabetic Wound Patients from Invasive Bacterial Infections by Innate Antimicrobial Peptides, and more.Additional chapters explore Bacteriophage Lysins as a potential therapeutic strategy for Methicillin Resistant Staphylococcus aureus infections, Structural insights and therapeutic potential of host defense peptides in combatting antimicrobial resistance, Antimicrobial protein as a cargo for antimicrobial nanomaterials to combat Gram-negative bacteria, Unravelling the Role of Antimicrobial Proteins in Leptospirosis: A Comprehensive Transcriptomic Analysis, and Deciphering Antimicrobial Peptide (AMP) Resistance Mechanisms in Enterococcus faecalis Through Integrated RNA-Seq and Hub Genes Identification.

Artificial Metalloproteins

1st Edition
Volume 723
December 1, 2025
Lisa Olshansky
English

In recent decades, artificial metalloproteins (ArMs) have emerged as powerful vehicles with which to control, implement, and leverage the unique reactivity and spectroscopic properties of their bound metal ions. This volume provides a practical guide to the latest ArM research, and is organized in a hierarchical fashion reflecting the primary, secondary, tertiary, and quaternary elements of protein structure. The first two sections provide a wide breadth of strategies for incorporating the primary coordination elements required for metal binding, which are further divided into the installation of heme and non-heme metallocofactor sites. The third section relates to the installation and tuning of secondary coordination sphere effects around the metal center to modulation of properties therein. Finally, the fourth section relates tertiary and quaternary protein design elements to resultant changes in properties of the bound metallocofactors. Ultimately the design, preparation, and characterization of ArMs at all of these hierarchical levels are needed to recapitulate the structures and functions of natural metalloproteins. By providing a detailed roadmap for achieving each of these elements, this volume aims to disseminate the cumulative knowledge developed by researchers around the world such that the next generation of ArM researchers can build from it and ultimately, beyond.

Advances in Biomembranes and Lipid Self-Assembly

1st Edition
Volume 42
December 1, 2025
Aleš Iglič︎ + 2 more
English

Advances in Biomembranes and Lipid Self-Assembly, Volume 42 highlights new advances in the field, with this new volume presenting interesting chapters written by an international board of authors.

Bacterial Efflux Systems

1st Edition
Volume 722
November 15, 2025
Ben Luisi + 1 more
English

Bacterial Efflux Systems, Volume 722 covers experimental and computational approaches to study the function, structure, and mechanism of bacterial efflux pump systems. The methods covered include preparation of samples for structural and functional analyses and in vivo activity analysis. Specific chapters in this release cover Efflux pump functional assays, NMR and functional analysis efflux pumps, Methods for saturation mutagenesis and functional characterization, Cooperation and functional interplay between pumps, Mapping interactions, HDX-MS, and much more.Other sections cover Recombinant Expression and purification, Reconstitution of a RND efflux pump in different artificial membranes for a structural biology approach, Reconstitution of rnd and mfs efflux pumps for structural biology, CusCBA system, Expression and purification of efflux pumps of Gram positives, Expression and purification of efflux pumps of archaea, ABC transporters: BmrA preparation for NMR and PatA/PatB in peptidisc, In situ structural analysis of efflux assemblies by cryoET, Molecular dynamics simulations, and so much more.

Lipid Analysis in Biology, Health, and Disease

1st Edition
November 1, 2025
Sanjoy K. Bhattacharya
English

Lipid Analysis in Biology, Health, and Disease bridges the gap between discovery science and clinical lipidology, empowering both scientific and medical communities to apply lipid analysis and understanding in new research, as well as diagnosis and management of diseases. Here, international experts in the field begin with the basic biology of the lipids, followed by methods and technology for study, and lipids in lab medicine and epidemiology. This book uniquely combines biology and analysis to drive healthcare advances, with a diverse range of technologies and analytic methods considered.Basic lipid biology chapters discuss membrane expansion, lysosomal positioning, mitochondrial biogenesis, lipid-protein interactions, molecular dynamics, cholesterol transport, immune activation and inflammation, lipid droplets, and gender differences in lipid metabolism. Each disease chapter incorporates basic lipid epidemiology, risk factors, and genetic associations.

Isothermal Titration Calorimetry in Enzymology

1st Edition
November 1, 2025
Anthony Mittermaier + 1 more
English
Paperback
9 7 8 0 4 4 3 2 1 8 4 8 4
eBook
9 7 8 0 4 4 3 2 1 8 4 9 1

Isothermal Titration Calorimetry in Enzymology: Techniques and Applications provides a thorough, practical overview of ITC as a productive and powerful tool for quantifying enzyme catalysis and interactions with substrates, products, cofactors, and inhibitors. Here, global experts in the methodology discuss the full capabilities of ITC and how it may be applied in research, medicine, and industry and offer a basis to continue leveraging ITC as its uses expand and evolve. This book bridges the gap between research and application by giving extensive technical details on cutting edge ITC techniques across a wide swath of both ITC kinetics measurements and ITC thermodynamic measurements, alongside case studies illustrating the depth of knowledge that can be obtained. Applications discussed include measuring binding kinetics by ITC, analyzing ITC kinetic data, in vivo enzyme kinetics by ITC, analyzing enzyme kinetics in crowded solutions, studying catalysis of biological substrates, interpreting ITC binding curves, ITC in drug discovery, ITC in the development of cancer therapeutics, ITC analysis of aminoglycoside resistance enzymes, studying enzyme interactions with toxic metals, using ITC to study ubiquitination, and ITC studies of glycoside hydrolases, among others.

Extracellular Mitochondria in Health and Disease

1st Edition
November 1, 2025
Carla Lopes + 1 more
English

Extracellular Mitochondria in Health and Disease covers this new and significant area of research, shedding light on their roles in intercellular communication and disease pathology. These tiny powerhouses, traditionally known for their intracellular functions, are now recognized for their extracellular activities that impact various physiological and pathological processes. The book delves into a range of topics, including cellular mechanisms of mitochondrial transfer, metabolic signaling, and the crosstalk between extracellular vesicles and mitochondria. It serves as a comprehensive guide for scientists, researchers, and students eager to explore these novel findings and their potential implications.Other sections examine the roles of extracellular mitochondria in immune regulation and inflammation, neurodegeneration and neuroprotection, and their therapeutic potential. By bridging gaps in existing literature, this resource offers up-to-date research and discoveries, providing valuable insights into how extracellular mitochondria could revolutionize medical advancements and improve patient outcomes.

Viral Proteases

1st Edition
Volume 58
November 1, 2025
Claudiu T. Supuran + 1 more
English

Viral Proteases, Volume 58 in The Enzymes series, presents a comprehensive overview of recent advances in the structural and functional understanding of viral proteases and their potential as drug targets. This new volume includes chapters covering a wide range of topics, including Viral proteases as targets for antiviral drugs, Structural biology of viral proteases, Computational approaches for designing viral protease inhibitors, The peptidomimetic approach for the design of viral protease inhibitors, HIV protease and its inhibition, and much more.Additional sections cover Hepatitis C virus (HCV) proteases: structure, function and inhibition strategies, Coronaviruses main proteases and their inhibitors, Coronaviruses papain-like proteases, Flaviviruses proteases (Zika, West Nile, Dengue), Bacteriophage endolysins, and Challenges for developing selective viral protease inhibitors as anti-infectives.

Related subjects

Bioenergetics

Biological transport

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Comparative biochemistry

Enzymology

Free radicals

Lipids and lipid metabolism

Methodology techniques and instruments in biochemistry

Nucleic acids

Peptides amino acids amines amides

Physical biochemistry

Life Sciences

Physical Sciences & Engineering

Social Sciences & Humanities

Health