Next Generation Sequencing
A Primer
- 1st Edition - September 1, 2014
- Latest edition
- Language: English
Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit cultu… Read more
Purchase options
Description
Description
Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit culture, this publication instructs readers on the science behind the technology and the analysis and application of data. The primer includes a needed pros-and-cons list, protocols, and a discussion of implementing applications.
Filling the gap in next generation sequencing literature, Next Generation Sequencing: A Primer provides needed instruction for early researchers, students and researchers outside of their expertise across the biological and biomedical sciences.
Key features
Key features
- Provides information on how technology works
- Discusses how and why to prepare samples
- Instructs on how to analyze data
- Covers basic techniques and history behind the kit culture
Readership
Readership
Students, graduate students, postdocs and researchers in molecular biology, biochemistry, and genetics and across the life and biomedical sciences
Table of contents
Table of contents
Introduction
Current next generation technologies, clonal approaches, single molecule approaches
Rationale: NGS enables new biological questions
Growth of cells for NGS (using yeast and cultured mammalian cells)
Nucleic acid extraction: DNA and RNA
PCR, gel electrophoresis and quantitative PCR
Overview of diverse types of NGS sequencing libraries.
Preparation of genomic DNA and RNA-seq libraries
Sequencer Operation: overview of diverse platforms
Sequencing on the MiSeq
Data extraction and analysis I: quality filters, trimming, demultiplexing and mapping
Data analysis II: genome viariants- SNPs, CNVs and rearrangements
Data analysis III: RNA seq analysis- gene expression, non-coding sequences, splicing
Data analysis IV: Data visualization and data sharing
Summary and report preparation
Applications of NGS
Perspectives: NEXT next generation technologies
Product details
Product details
- Edition: 1
- Latest edition
- Published: September 1, 2014
- Language: English